The pore-forming alpha subunits of many ion channels are associated with auxiliary subunits that influence channel expression, targeting, and function. Several different auxiliary (beta) subunits for large conductance calcium-dependent potassium channels of the Slowpoke family have been reported, but none of these beta subunits is expressed extensively in the nervous system. We describe here the cloning and functional characterization of a novel Slowpoke beta4 auxiliary subunit in human and mouse, which exhibits only limited sequence homology with other beta subunits. This beta4 subunit coimmunoprecipitates with human and mouse Slowpoke. beta4 is expressed highly in human and monkey brain in a pattern that overlaps strikingly with Slowpoke alpha subunit, but in contrast to other Slowpoke beta subunits, it is expressed little (if at all) outside the nervous system. Also in contrast to other beta subunits, beta4 downregulates Slowpoke channel activity by shifting its activation range to more depolarized voltages and slowing its activation kinetics. beta4 may be important for the critical roles played by Slowpoke channels in the regulation of neuronal excitability and neurotransmitter release.
After reconstruction of a highway section with three wildlife underpasses, but only limited wildlife exclusion fencing, elk (Cervus canadensis)-vehicle collisions (EVC) increased 21%. We retrofitted an existing 1-m right-of-way fence along 4.2 km, raising it to 2.2 −2.4 m in height and tying it into underpasses at the project's east end. With no logical western fence terminus, we installed an animalactivated detection system (AADS) and motorist alert signage at a designated at-grade crosswalk to prevent collisions when animals crossed. Our goal was to achieve modified motorist behavior without long-term habituation while allowing wildlife to cross via the crosswalk, promoting highway safety and landscape connectivity. Beforeproject EVC (9.33/year) declined 97% after the new fencing. Our AADS achieved reduced vehicle speeds (13%) and increased motorist alertness (5.5-fold increase) with signs activated. Average speed reduction and braking response remained significantly higher with sign activation across all 9 years of our evaluation. Thus, our placeand time-specific AADS design successfully modified motorist behavior without habituation.
Over many years, numerous agency biologists, wardens, students, and postdocs have contributed thousands of hours into planning, collecting, and analyzing the data that pertain to each of the herds described in this report. Many staff and students with Infographics Lab at the University of Oregon Department of Geography were involved in map design and production. These included research assistant Joanna Merson, and the following students:
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