OBER EINIGE INHALTSSTOFFE DER UNTERIRDISCHEN T E I L E V O N NARDOSTACHYS CHINENSIS BATALINDie unterirdischen Teile von Nardostachys chinensis B a t a 1 i n (Valerianuceae) werden in der chinesischen Volksmedizin therapeutisch in ähnlicher Weise verwendet wie die Nardostaclzys jatamansi d e C a n d o 11 e in Indien, die sich durch eine hypotensive, antiarrhytmische und sedative Wirkung auszeichnet (Literatur: R ü c k e r und G 1 a u c h , 1967). Während über die Inhaltsstoffe von N . jatamansi schon einige Untersuchungen vorliegen, fehlen bisher diesbezügliche Kenntnisse von der N. chinensis.Es standen für die Untersuchungen 100 kg der handelsüblichen, lufttrockenen, nicht zerkleinerten Droge der unterirdischen Teile von Nnrdosiaclzys chinensis zur Verfügung, die aus der Provinz Szetschuan der Volksrepublik China stammte. Die Droge stimmte im Aussehen und in den Merkmalen mit der Beschreibung von B a t a l i n (1894) und den Angaben der 1961 erschienenen chinesischen "Drogenkundeu (H s ü K U o -C h ü n U. Mitarb.) überein. Entgegen den Angaben der älteren Autoren hält es W e b e r l i n g2 jedoch nicht für möglich, daß N. d~inensis als eigene Art gegen den sehr variablen Formenkreis von N. jalamcmsi abzugrenzen ist.In die Untersuchungen mit einbezogen wurden: 1 . Drei aus verschiedenen indischen Quellen stammende Proben der handelsüblichen, lufttrod
The absorption and elimination of radioactivity after the oral or intraperitoneal administration of[4-14C]mofebutazone was studied in rats. The blood concentration of radioactivity reached a maximum after about 0.7 h, fell rapidly until about 2 h, and then declined slowly. There was sometimes a second peak between 3-6 h. Elimination of radioactivity in urine and feces was extensive and rapid. Over a 24 h period, 73% of the orally administered radioactivity was eliminated in the urine and 15% in the faeces; most of this was eliminated during the first 8 h (89% of the urine radioactivity, 56% of the faeces radioactivity). In anaesthetized rats with cannulated bile ducts, 94% of the intraperitoneally injected radioactivity was eliminated in the bile over a 6 h period. Most of the radioactivity (about 85%) eliminated in the bile and the urine was in the form of a glucuronide and only small amounts less than 10%, was in the form of mofebutazone.
Wiihrend sich bei der Kondensation von bhlomiurediathylester mit Propin-(2)-yLharnstoff in aegenwart von Magneaiumiithylat N-Pmpinylbarbitursiiure bildet, entabhen BUS snbstituiertm Maloneiiureestern unbr den gleichen Bedingungen 7-Alkyl-bzw. Aryl-2-methyl-oxazolino-(3,2-c)-uracile. N-Propinyl-benzoeeiiurearnid cyolisiert erst unter dem EinfluB von konz. Schwefelsiiure zum 2-Phenyl-6-methyl-oxazol und beim Erhitzen mit Phosphorpentasulfid zum 2-Phenyl-6-methyl-thiszol.Wie vor einiger Zeit gezeigt werden konntel), liidt sich die B-Propin-(2')-yl-barbitursiiure mit konz. Schwefelsiiure oder Phosphorsaure in Furo-(2,3-d)-uracil iiberfuhren. Es achien nun interemant zu untersuchen, ob auch die N-Propinyl-barbitursiiure (I) diesen intramolekularen RingschluD eingeht ; es muate das 2-Methyl-oxazolino-(3,2-o)-uracil (11)
The polymerase chain reaction (PCR) using primers designed based on the sequence information reported (Genbank accession number M94319) was used for detection and quantitation of Liberobacter asiaticum, the bacterium associated with citrus Huanglongbing (Greening disease). By this procedure, the presence of L. asiaticum were detected in samples of infected citrus, periwinkle and the psylla vector fed on infected plants. To compare the amount of the bacteria present in different samples, a PCR-based quantitation was developed in which a competitor DNA fragment was serial-diluted and co-amplified with the sample DNA in the same reaction tube. The competitor shares the primer binding sites with the liberobacter DNA and competes for the primers and the limited substrates during the PCR reaction. The PCR products were separated by agarose gel electrophoresis and the banding intensity was recorded by densitometry. The amount of PCR products from the liberobacter DNA is inversely proportional to that of the competitor, making it possible to construct a standard curve. The quantity of liberobacter was deduced from the standard curve by linear regression analysis.
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