We have found CueO from Escherichia coli to have a robust cuprous oxidase activity, severalfold higher than any homologue. These data suggest that a functional role for CueO in protecting against copper toxicity in vivo includes the removal of Cu(I).
Background:The multicopper oxidase CueO allows Escherichia coli to survive in aqueous solutions with a high copper concentration. Results: Cu(I) and Ag(I) ions coordinate to a flexible, methionine-rich sequence.
Conclusion:The methionine-rich insert and a substrate-binding site ensure that only Cu(I) is oxidized. Significance: Understanding how bacteria survive in the presence of normally toxic concentrations of metal ions may lead to better antibacterial agents.
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A metal-free room temperature decarboxylative cross-coupling between cinnamic acids and arylsulfonyl hydrazides has been realized for the first time for the synthesis of (E)-vinyl sulfones. The scope and versatility of the reaction has been demonstrated by the regio- and stereoselective synthesis of 22 derivatives with diverse structural features.
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