Pollen germination forms one of the most important stage post pollination prior to fertilization. This is essential for proper seed setting and seed development. In vitro pollen germination test is the most reliable way of assessing the pollen viability. In the present study pollen grains of seven genera under Apocynaceae family namely, Allamanda, Alstonia, Catharanthus, Nerium, Plumeria, Thevetia and Tabernaemontana were tested in some basic cultural media, such as Brewbaker's media, 6% Glucose solution, 4% Calcium Nitrate solution and 3% Boron solution.Alstonia pollen grains exhibited highest percentage of germination rate in all the cultural media.Glucose and Brewbaker's media is found to be highly suitable for efficient pollen germination in all the genera. Boron solution is effective for germination of pollen grains of tree species. In vitro pollen germination can be easily carried out in laboratories. These results can be utilised in plant breeding programmes to improve cultivar and varieties.
Taxonomy and identification of angiosperms are mainly based on external morphological characters and traits of the plants; however palynological data sets are remotely used for the study. Pollen characters are genetically regulated that can be used as an ideal tool for establishing taxonomic groups. This type of integrated study can be useful in redefining the status of families in higher group of plants. Apocynaceae, a Eurypalynous family exhibits variation in possessing more than one type of pollen grains from simple porate to compound colporate apertures. Pollen attributes has been utilized in building a pollen key that would enable us to distinguish genera solely on the basis of pollen characters.
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