Improvised Media for In Vitro Pollen Germination of some Species of Apocynaceae

Chatterjee, Reshmi; Sarkar, Satadip; Rao, GM Narasimha

doi:10.3126/ije.v3i3.11074

Cited by 9 publications

(8 citation statements)

References 17 publications

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“…Pollen grains are produced in profuse number per anther to ensure successful pollination followed by germination that eventually led to seed-set production (Chatterjee et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Pollen Morphology and Palynotaxonomical Studies of Common Garden Plants of Apocynaceae, In Kolkata, West Bengal, India

Chatterjee¹,

Sarkar²,

Rao

2014

Int. J. Environ.

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Taxonomy and identification of angiosperms are mainly based on external morphological characters and traits of the plants; however palynological data sets are remotely used for the study. Pollen characters are genetically regulated that can be used as an ideal tool for establishing taxonomic groups. This type of integrated study can be useful in redefining the status of families in higher group of plants. Apocynaceae, a Eurypalynous family exhibits variation in possessing more than one type of pollen grains from simple porate to compound colporate apertures. Pollen attributes has been utilized in building a pollen key that would enable us to distinguish genera solely on the basis of pollen characters.

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“…Pollen grains are produced in profuse number per anther to ensure successful pollination followed by germination that eventually led to seed-set production (Chatterjee et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Pollen Morphology and Palynotaxonomical Studies of Common Garden Plants of Apocynaceae, In Kolkata, West Bengal, India

Chatterjee¹,

Sarkar²,

Rao

2014

Int. J. Environ.

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“…In wheat, pollen viability is mostly determined by pollen staining methods and within 30 minutes at room temperature or within few hours when stored at 4°C [7, 8, 9, 10, and 11]. In vitro pollen germination tests are performed mostly on fresh pollen grains since after shedding pollen grains remain alive for 30-40 minutes at room temperature or few hours when preserved at 4°C [12,11]. High quality crops also depend on the strength of the pollen and it is the most important factor in the breeding program [13].…”

Section: Introductionmentioning

confidence: 99%

A simple method for preserving pollen viability and longevity in wheat

Khan

Sajjad

2023

Preprint

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Wheat pollen grains have very short longevity and are non-viable after ~30 minute at room temperature and ~60 minutes at 4°C. Pollen grain viability can be prolonged to ~24 hrs with existing anther preservation methods. Herein, we developed a new and very simple method of spike preservation to prolong pollen viability up to 1 week. The present study was evaluated to determine the effect of storage temperature and duration on the viability and in vitro germination of pollen grains of 50 spring wheat genotypes from two preservation methods of pollens viz, Anther preservation method and Spike preservation method. Pollens collected from both methods were stored at room temperature (22°C), fridge (4°C) for 0 and 1 week. Pollen viability were assessed using Alexander staining techniques with both methods at storage conditions 22°C and 4°C. In vitro germination was determined using liquid germination medium with both methods at 4°C. The results revealed that the pollen viability and in vitro germination as determined by Spike preservation method was highest when pollens were stored at 4°C up to 1 week. In addition, in vitro pollen germination and pollen viability significantly reduced as storage duration increased. It was recorded, that no pollen germination was observed under anther preservation method at storage conditions 4°C after 1 week while 2 to 14% pollen germination was obtained by spike preservation method only at 4°C up to 1 week. Based on these results, it is suggested that a storage temperature of 4°C by Spike preservation method for wheat pollen storage is good. The present study would be useful to develop a protocol for the storage of pollen for further use in the conservation and breeding of wheat.

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Section: Introductionmentioning

confidence: 99%

“…Further, Andronescu [23] reported that Graminaceous taxa's pollen grains lose viability very quickly, and under ideal storage conditions, successful preservation was thought to only last for around 10 days [24] However, Li Xun Zhen et al [25] preserved rice pollen grains at low temperature for 15 days. For measuring pollen germination vigour and percentage over time, in vitro techniques have been used [12,11]. A genotype can be regarded a good pollinators if its pollen viability is high, and examination of pollen viability and pollen tube formations are crucial criteria for pollen evaluation [26].…”

Section: Introductionmentioning

confidence: 99%

Spike preservation: A simple method to preserve pollen viability and in vitro germination in wheat

Khan

Sajjad

2023

Preprint

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Background Wheat pollen grains have very short longevity and are non-viable after ~30 minute at room temperature and ~60 minutes at 4°C. Pollen grain viability can be preserved maximum to ~24 hrs with existing preservation methods. Results Herein, we developed two simple methods-anther preservation and spike preservation- to preserve the pollen grain viability in wheat. The methods were validated using viability and in vitro germination of pollen grains of 50 diverse spring wheat genotypes. Anthers and spikes were collected for anther preservation and spike preservation methods, respectively, and stored at room temperature (22°C) and fridge (4°C) for 0 and 1-week. Pollen viability were assessed using Alexander staining techniques at two storage temperatures 22°C and 4°C. In vitro germination was determined using liquid germination medium at 4°C. After 1 week, the pollen viability and in vitro germination as determined with Spike preservation method were higher as compared with those of anther preservation method at 4°C. In addition, in vitro pollen germination and pollen viability significantly reduced as storage duration increased. After 1-week, the pollen grains preserved with anther preservation method at 4°C failed to germinate. Conclusion The Spike preservation method is effective for preserving the pollen grain viability and in vitro germination in a large panel of wheat genotypes. This new method is instrumental to further our understanding on pollen grain viability and germination.

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Improvised Media for In Vitro Pollen Germination of some Species of Apocynaceae

Cited by 9 publications

References 17 publications

Pollen Morphology and Palynotaxonomical Studies of Common Garden Plants of Apocynaceae, In Kolkata, West Bengal, India

Pollen Morphology and Palynotaxonomical Studies of Common Garden Plants of Apocynaceae, In Kolkata, West Bengal, India

A simple method for preserving pollen viability and longevity in wheat

Spike preservation: A simple method to preserve pollen viability and in vitro germination in wheat

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