No abstract

Microplastics can have direct physical effects on organisms in freshwater systems, and are considered as vectors for absorbed environmental pollutants. It is still under discussion if microplastics are relevant pollutant vectors for uptake into aquatic organisms in comparison to further uptake pathways, e.g., via water or sediment particles. We analyzed how the presence of microplastics (polyamide particles, PA) modifies acute effects of the environmental pollutant bisphenol A (BPA) on freshwater zooplankton (Daphnia magna). Daphnids were exposed to PA particles and BPA alone, before combining them in the next step with one concentration of PA and varying concentrations of BPA. The PA particles themselves did not induce negative effects, while the effects of BPA alone followed a typical dose-dependent manner. Sorption of BPA to PA particles prior to exposure led to a reduction of BPA in the aqueous phase. The combination of BPA and PA led to decreased immobilization, although PA particles loaded with BPA were ingested by the daphnids. Calculations based on physiochemistry and equilibrium assumptions indicated lower BPA body burden of daphnids in the presence of PA particles. These results confirm model-based studies, and show that investigated microplastic concentrations are negligible for the overall pollutant uptake of daphnids with water as additional uptake pathway.

Currently, there is a trend toward an increasing use of biopesticides assumed to be environmentally friendly, such as Bacillus thuringiensis (Bt). Studies of the Bt toxicity to nontarget organisms have reported low effects at high exposure levels, which is interpreted as indicating negligible risk to nontarget organisms. We investigated the response of the nontarget organism Daphnia magna to waterborne DiPel ES, a globally used Bt formulation. Neonates and adults were exposed for 48 h to a wide range of concentrations, and immobilization and mortality were monitored. Whole body biomarkers (body weight, protein, chitobiase, catalase, xenobiotic metabolism, and acetylcholinesterase) were measured in the adults. The immobilization and mortality of the neonates were affected in a nonmonotonic and inverted U-shaped pattern with ECs that were ∼10-fold lower than those reported by the manufacturer. The immobilization of adults demonstrated a similar pattern, but significant mortality was not observed. The biomarker results revealed multiphasic dose-response curves, which suggested toxicity mechanisms that affected various physiological pathways. The main particle size in exposure media was in the size range of bacterial spores and crystal toxins. However, the chemical heterogeneity was nonmonotonic, with a change in the phase at the maximum of toxicity (∼5 μL L), which might explain the observed nonmonotonic effects. These results demonstrate the vulnerability of a nontarget organism to a biopesticide that is considered to be safe, while challenging the universal applicability of the central ecotoxicological assumption of monotonicity.

Detailed knowledge about claw formation and growth rate is a prerequisite for the interpretation of avian claw stable isotopes, as is commonly done with feather stable isotopes to e.g. infer habitat use, dietary specialisations, and spatial occurrence. In this study, we provide basic information about claw formation and empirical evidence about the time scale of archiving isotopic information to develop a reliable assessment of archived isotopic pattern in claws of passerines. Avian claws grow conically from the tip of the bone of the phalanx. The length of the tip of an avian claw, suitable for stable isotope analysis, is about 42 ± 6.8% (SD) of total linear claw length and can also be estimated from the body mass of a given species. Claw growth rate in adult songbirds typically ranged between 0.03 and 0.05 mm d−1, but differed between front and back toes, and varied seasonally. From the claw growth rate, the archiving period of a given claw length can be estimated. In long‐distance migrant species, δ13C of claws matched δ13C of feathers grown during the same period (wintering or breeding period). In Palaearctic‐African migrants sampled in the breeding season, δ13C of the distal half of the claw tip reflected the African wintering site, while the proximal half reflected a blend of African and European δ13C signatures, as expected. Hence there is empirical evidence that entire claw tips mirror the isotopic environments over longer periods (up to months), and over weeks when parts can be analysed. However any part of a claw contains a blend of material formed at different times due to the claw's conical (i.e. longitudinal and lateral) growth. Avian claws provide a complementary isotope archive for investigations, but its applicability may vary according to the ecology of the study species.

The plant genus Ficus is a keystone resource in tropical ecoystems. One of the unique features of figs is the diversity of fruit traits, which in many cases match their various dispersers, the so-called fruit syndromes. The classic example of this is the strong phenotypic differences found between figs with bat and bird dispersers (color, size, presentation, and scent). The ‘bird-fig’ Ficus colubrinae represents an exception to this trend since it attracts the small frugivorous bat species Ectophylla alba at night, but during the day it attracts bird visitors. Here we investigate day to night changes in fruit scent as a possible mechanism by which this ‘bird-fig’ could attract bats despite its fruit traits, which should appeal solely to birds. Analyses of odor bouquets from the bat- and bird-dispersal phases (i.e. day and night) differed significantly in their composition of volatiles. We observed a significant increase in relative amounts of sesquiterpene and aromatic compounds at night while relative amounts of two compounds of the fatty acid pathway were significantly higher during day. This finding raises the question whether Ficus colubrinae , a phenotypically classic ‘bird-fig’, might be able to attract bat dispersers by an olfactory signal at night. Preliminary observations from feeding experiments which indicate that Ectophylla alba is capable of finding ripe figs by scent alone point in this direction. However, additional behavioral experiments on whether bats prefer the ‘night-bouquet’ over the ‘day-bouquet’ will be needed to unequivocally answer this question.

The plant genus Ficus is a keystone resource in tropical ecoystems. One of the unique features of figs is the diversity of fruit traits, which in many cases match their various dispersers, the so-called fruit syndromes. The classic example of this is the strong phenotypic differences found between figs with bat and bird dispersers (color, size, and presentation). The ‘bird-fig’ Ficus colubrinae represents an exception to this trend since it attracts the small frugivorous bat species Ectophylla alba at night, but during the day attracts bird visitors. Here we investigate the mechanism by which this ‘bird-fig’ attracts bats despite its fruit traits, which should appeal solely to birds. We performed feeding experiments with Ectophylla alba to assess the role of fruit scent in the detection of ripe fruits. Ectophylla alba was capable of finding ripe figs by scent alone under exclusion of other sensory cues. This suggests that scent is the main foraging cue for Ectophylla alba. Analyses of odor bouquets from the bat- and bird-dispersal phases (i.e. day and night) differed significantly in their composition of volatiles. The combination of these two findings raises the question whether E. alba and F. colubrinae resemble a co-adaptation that enables a phenotypically classic ‘bird-fig’ to attract bat dispersers by an olfactory signal at night thus maximizing dispersal.

No abstract