Eleocharis dulcis is a swamp plant has potential as a traditional medicine. The purpose of this study was to compare the results of determining specific and non-specific parameters on simplicia and methanol extract of purun tikus’s stems. Determination of specific parameters includes organoleptic, macroscopic - microscopic tests, water soluble extract content, ethanol soluble extract content and phytochemical screening such as testing for alkaloids, flavonoids, tannins, saponins and steroids/triterpenoids. Determination of non-specific parameters includes drying shrinkage, determination of total ash content, acid insoluble ash content and heavy metal contamination. The results of the study on the specific parameters of stem extract showed that the simplicia powder was light brown in color, had a characteristic odor that was not pungent and had a bitter taste and on microscopic examination only the simplicia could be observed epidermis, transport bundles, parenchyma and collenchyma fragments,. The results of the non-specific simplicia characterization test showed drying shrinkage of 8.91%, total ash content of 13.79%, acid insoluble ash content of 6.89%. The results showed that both simplicia and methanol extract of purun tikus’s stems met the general requirements for specific and non-specific characteristics except for the total ash content of the simplicia which was declared not eligible.
Kanker adalah penyakit mematikan dan angka kejadiannya di Indonesia sangat tinggi. Data di Departemen Kesehatan diperoleh angka 1,8 per 100 ribu penduduk. Ironisnya saat ini belum ditemukan antikanker yang efektif dan aman. Minyak kayu manis yang diperoleh dari tanaman kayu manis (Cinnamomum burmannii) diduga memiliki aktivitas antikanker. Penelitian sebelumnya bertujuan untuk isolasi, standarisasi minyak kayu manis sudah dilakukan. Saat ini penelitian dilanjutkan terhadap uji aktifitas serta keamanan minyak kayu manis untuk mendapatkan fitofarmaka antikanker. Pengujian aktivitas antikanker dengan menguji kemampuan penghambatan sel kanker terhadap sel line kanker WiDr (kanker kolon). Keamanan minyak kayu manis ditentukan dengan melakukan uji toksisitas sub kronis terhadap mencit putih jantan. Hasil penelitian ini menunjukkan bahwa minyak kayu manis memiliki aktifitas sitotoksik terhadap kultur sel WiDr dengan IC 50 =13,70 µg/mL. Pemberian minyak kayu manis kepada hewan uji tikus selama 1 bulan tidak memberikan perubahan biokimia darah BUN kreatinin, SGPT dan SGOT, serta hematologi Darah. Hasil histopatologi terhadap hepar dan ginjal juga tidak memberikan kerusakan sel kecuali pada dosis 0,04 mL/ 200 gBB.
Wound healing is a repair process of damaged skin tissue caused by injury. The bilimbi (Averrhoa bilimbi L.) plant is widely known to have potential in wound healing and the plant part that is commonly used is the leaves. The purpose of this study was to determine the wound healing effects of bilimbi leaf extract (Averrhoa bilimbi L.) on rabbits (Oryctolagus cuniculus) topically. The wound healing test was conducted using the New Zealand strain rabbit test animal. The rabbit’s back skin was induced with 8 mm diameter wound and the induced wounds were grouped into 5 groups. The groups included a negative control group that was given with aqua dest solution; the positive control group was given with betadine solution; treatment groups were given with 3%; 6%; and 12% bilimbi leaves extract. The wounds that were treated with betadine solution and bilimbi leaves extract 3% healed on day 13th. The wounds that were treated with bilimbi leaves extract 6 and 12% were healed on day 12nd. The different results showed by the wounds that were treated with aqua destilata which were healed on day 14th. The results showed that bilimbi leaf extract was topically effective in wound healing on rabbit test animals. The most effective concentration of bilimbi leaves extract in wound healing was 6% and 12%.
INTISARI Beberapa penelitian membuktikan ekstrak dan infus daun salam sebagai antidiare. Fraksinasi dapat menyederhanakan senyawa dalam ekstrak. Penelitian ini bertujuan untuk mengetahui aktivitas fraksi air ekstrak etanol daun salam (FAEEDS) sebagai protektor diare yang diinduksi castor oil, antimotilitas usus, dan mengetahui keberadaan flavonoid dan tanin didalamnya. Penelitian ini merupakan penelitian eksperimental menggunakan randomized matched posttest only control group design. FAEEDS dosis 1, 2, dan 3 g/kgBB diberikan pada mencit secara per oral. Kelompok kontrol positif yang digunakan adalah loperamid HCl 0,5 mg/kgBB dan PGA 3% 25 ml/kgBB diberikan pada kelompok kontrol negatif. Satu jam kemudian, mencit diberi castor oil secara per oral. Banyaknya feses cair dan tidak terbentuk yang terbentuk selama 4 jam diamati. Pengujian aktivitas antimotilitas dilakukan dengan memberikan suspensi norit 10% 10 ml/kgBB 45 menit setelah pemberian castor oil. Satu jam kemudian dilakukan pengukuran panjang usus yang dilalui marker norit dan panjang usus seluruhnya. Data banyaknya feses yang cair dan tidak berbentuk serta data rasio panjang usus yang dilalui norit diuji perbedaannya dengan uji Mann-Whitney dengan taraf kepercayaan 95%. Keberadaan flavonoid dan tanin diketahui melalui kromatografi lapis tipis. Hasil penelitian menunjukkan FAEEDS beraktivitas sebagai protektor diare dan antimotilitas usus pada semua dosis. Flavonoid dan tanin terkandung dalam FAEEDS. Kata kunci: fraksi air, daun salam, protektor diare, antimotilitas, castor oil, loperamid HCl ABSTRACT Some research proves extracts and infusion of salam leaf as antidiarrheal. Fractination can simplify the compounds in the extract. The aim of this research is to know the diarrhea protector activity of water fraction of ethanol extract of salam leaf (WFEESL), the antimotility activity, and to verify the persence of flavonoids and tannins in the fraction. The research was an experimental study using randomized matched posttest only control group design. WFEESL doses 1, 2, and 3 g/kgBW were given to mice orally. The positive control group was given loperamide HCl 0.5 mg/kgBW. One hour later, each mouse was given 1 ml of castor oil orally. Observations were made on the weight of liquid and unformed stools that formed during 4 hours of observation. Ten percent of carbo adsorbent suspensian 10 ml/kgBW was given orally as marker to the mice in the group of antimotilily activity 45 minutes after castor oil. The parameter which measure was the length of intestinal which passed by carbo adsorbent compared with the whole intestinal length. The data analyzed by Kruskal Wallis test, continued with Mann Withney test, with 95% of confidence level. The presence of flavonoids and tannins are known by thin layer chromatography. The results showed that WFEESL exhibits protective diarrhea and antimotility activity at all doses. Flavonoids and tannins are contained in WFEESL. Keywords: water fraction, salam leaves, diarrhea protector, antimotility, castor oil, loperamide HCl
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