Abstract. Fachrial E, Anggraini S, Harmileni, Nugroho TT, Saryono. 2019. Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia. Biodiversitas 20: 3493-3498. The application of enzymes industrially is increasing every year and thermophilic microbes are a promising source of these enzymes for industrial use due to their temperature stability. The aim of this study, therefore, was to isolate, characterize and identify the enzyme producer-thermophilic bacteria from the Penen Hot Spring in Deli Serdang, North Sumatra, Indonesia. In the experimental setup, carbohydrase activities including inulinase and amylase were determined by the formation of clear zone around the colonies after soaking with Lugol on the TSA medium supplemented with 1% inulin powder and 1% starch. Similarly, the protease activities were determined by the formation of clear zone around the colonies on Skim Milk Agar Medium. Then, of the 11 isolates, only one known as UTMP 12 showed maximum enzyme activity. The isolate was then characterized based on morphology and biochemistry and found to be Bacillus subtilis strain JCM 1465 (accession number NR_113265, homolog 99.72%). Furthermore, this is the first study on carbohydrase and protease activities of Bacillus subtilis strain JCM 1465 and the result shows that the thermophilic bacteria are needed in the production of carbohydrase and protease.
Abstract. Fachrial E, Putri RRJS, Lister INE, Anggraini S, Harmileni, Nugroho TT, Saryono. 2020. Molecular identification of cellulase and protease producing Bacillus tequilensis UTMSA14 isolated from the geothermal hot spring in Lau Sidebuk Debuk, North Sumatra, Indonesia. Biodiversitas 21: 4719-4725. The industrial need for stable microbial enzymes tends to increase every year. The aim of this study was to isolate and identify the protease and cellulase producing thermophilic bacteria isolated from the geothermal spring of Lau Sidebuk Debuk, North Sumatra, Indonesia. The protease activity was determined based on the formation of halo zone on Skim Milk Agar, while cellulase was confirmed using CMC plate agar method. Nine bacterial isolates were successfully obtained from the sediments and water, and both activities were observed in only one isolate, known as UTMSA14. These isolates were characterized biochemically and morphologically. The molecular identification of UTMSA14 was done by 16S rRNA sequencing based on BLAST and phylogenetic analysis using MEGA X. Phylogenetic analysis showed that UTMSA14 clustered together with Bacillus tequilensis strain 10b (accession number NR_104919.1), which ascertained the isolate as Bacillus tequilensis strain 10b (accession number NR_104919.1). This is the first report of a thermophilic bacteria isolated from Lau Sidebuk Debuk hot springs, with the ability to produce cellulase and protease. further research is needed to purify and characterize the resulting enzymes, with a potential for industrial applications.
Peningkatan konsentrasi CO2 ke atmosfer menjadi salah satu penyebab pemanasan global akibat efek gas rumah kaca, Perluasan perkebunan kelapa sawit, terutama bila mengonversi hutan, berpotensi menyebabkan peningkatan emisi gas rumah kaca (GRK). Tanaman kelapa sawit yang merupakan tanaman tahunan yang berpotensi dalam penyerapan emisi karbon. Penelitian dilakukan di perkebunan kelapa sawit lahan berpirit PT. Mopoli Raya bagian afdeling I Damar Condong. Rancangan penelitian ini menggunakan rancangan deskriptif dengan metode sampling tanpa pemanenan (non-destructive sampling) untuk pengukuran biomassa pohon hidup, pohon mati, dan kayu mati dan metode sampling dengan pemanenan (destructive sampling) untuk pengukuran biomassa tumbuhan bawah dan serasah. Pengamatan pada plot-plot contoh sesuai dengan asal tipe bibit kelapa sawit bersertifikat. Penelitian akan dilaksanakan pada Agustus – September 2020, dengan plot ukuran 20 m x 60 m sebanyak dua kali ulangan pada kelapa sawit kategori dewasa dengan tegakan umur 15-20 tahun dan kategori tua dengan tegakan umur besar dari 20 tahun. Cadangan karbon lahan berpirit semakin besar bila umur sawit dalam kondiisi produktif.
Stored carbon is present in plant stands by measuring plant biomass. The amount of carbon stock depends on the diversity and density of existing plants, soil types and management methods. The purpose of this study was to determine whether or not there is an effect of differences in pyrite land on the size of carbon sequestration in oil palm plantations in the Production Plant (TM) phase (<20 years). This research was conducted in May - September 2020. The research sample was taken at the oil palm plantation of PT. Mopoli Raya, part of payarambe, North Sumatra. The test and calculation of carbon stocks were carried out at the Laboratory of PPKS Medan North Sumatra. The design of this study used a descriptive design, namely the non-destructive sampling method for measuring the biomass of living trees, dead trees and dead wood and the sampling method by harvesting (destructive sampling) for measuring the biomass of understorey plants and litter. Observations on sample plots according to plant age class with pyrite planting conditions. From the results of this research, oil palm (Elaeis guineensis Jacq) can be concluded that the carbon stock of pyrite land is greater.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.