in comparison with controls under severe water stress conditions. We summarize the extent to which the dual and multiple combinations of microorganisms can overcome challenges related to drought by enhancing plant physiological responses.

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Background: Agricultural production is challenged by the limitation of non-renewable resources. Alternative fertilizers are promoted but they often have a lower availability of key macronutrients, especially phosphorus (P). Biological inoculants, the so-called bio-effectors (BEs), may be combined with these fertilizers to improve the nutrient use efficiency. Methods:The goal of this study was to assess the potential of three BEs in combination with alternative fertilizers (e.g., composted manure, biogas digestate, green compost) to promote plant growth and nutrient uptake in soils typical for various European regions. Pot experiments were conducted in Czech Republic, Denmark, Germany, Italy, and Switzerland where the same variety of maize was grown in local soils deficient in P in combination with alternative fertilizers and the same set of BEs (Trichoderma, Pseudomonas, and Bacillus strains). Common guidelines for pot experiment implementation and performance were developed to allow data comparison, and soils were analyzed by the same laboratory.Results: Efficiency of BEs to improve maize growth and nutrient uptake differed strongly according to soil properties and fertilizer combined. Promising results were mostly obtained with BEs in combination with organic fertilizers such as composted animal manures, fresh digestate of organic wastes, and sewage sludge. In only one experiment, the nutrient use efficiency of mineral recycling fertilizers was improved by BE inoculation. Conclusions:These BE effects are to a large extent due to improved root growth and P mobilization via accelerated mineralization.

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The majority of soil organic nitrogen (N) is bound in protein-like compounds and therefore its proteolysis in peptides and amino acids is considered the initial and rate limiting step of N mineralization. Proteolysis of N bound in organic fertilizer and subsequent provisioning for crops is a central element in agro-ecological intensification. Long-term farming system effects on N provisioning from organic fertilizer to crops and its underlying functional microbial communities were analyzed in experiments conducted in soils from the "DOK" system comparison trial (bio-Dynamic, bio-Organic, and "Konventionell") subjected to optimal and future projected drought scenarios. A plant nutrition experiment using 15 N labeled lupine as a fertilizer (green manure) identified 30% higher amounts of N derived from fertilizer in ryegrass grown on organically compared to conventionally managed soil, but only when subjected to dry conditions. A second experiment, also amended with lupine green manure, assessed the effect of farming system and drought stress on N cycling microbes with a focus on alkaline (apr) and neutral (npr) metallopeptidase encoding microbial communities. apr encoding microbial communities were more strongly affected by farming system and water treatment than npr encoding communities. Differences in structure and diversity of apr encoding microbial communities showed concomitant patterns with distinct N provisioning from organic fertilizer in the plant nutrition experiment. It is suggested that conventionally managed systems are less capable in maintaining diversity and initial structure of apr encoding microbial communities when subjected to drought scenarios. Overall, we demonstrated organically managed soils to provide a more stable N provisioning potential from organic fertilizer under future drought scenarios, likely facilitated by a distinct and more adaptive proteolytic microbial community. This work contributes to an in-depth comprehension of yet poorly studied fundamental soil processes and helps developing strategies to maintain a versatile and functioning microbial community in a rapidly changing environment.

Drought is a limiting factor for crop production, especially in arid and semi-arid climates. In this study, Sorghum bicolor plants were inoculated, or not, with Rhizophagus irregularis, an arbuscular mycorrhizal (AM) strain typical for temperate climates, or Rhizophagus arabicus, a strain endemic to hyper-arid ecosystems. Plants were grown under well-watered or drought conditions in compartmented microcosms. Transpiration rates, plant growth, and nutrient uptake (using N as a tracer) were determined to assess the impact of drought stress on sorghum plants in AM symbiosis. Although AM colonization did not affect the bulk biomass of host plants, R. arabicus improved their transpiration efficiency and drought tolerance more than R. irregularis. Moreover, R. arabicus was able to extract moreN from the soil under both water regimes, and AM-driven enhancement of the nitrogen and phosphorus content of sorghum, especially when water was limiting, was greater for R. arabicus-inoculated plants than for R. irregularis-inoculated plants. Our work demonstrates close links between AM hyphal phosphorus and nitrogen transport and uptake by AM plants for both AM fungal species. It also underscores that, under the drought stress conditions we applied, R. arabicus transfers significantly more nitrogen to sorghum than R. irregularis.

Three new species of arbuscular mycorrhizal (AM) fungi (Glomeromycota) were isolated from soil samples collected from a hyperarid sandy plain of South Arabia. Morphological characteristics of the spores clearly differentiated them from closely related AM species. Molecular analyses were performed on rDNA sequences obtained from single spores including a ~1700 bp region comprising partial SSU, ITS, partial LSU and the ~600 bp ITS region only. The phylogenetic trees based on these regions showed that the three species belong to well described genera but are clearly distinct from known species. Consequently, we describe them here as Diversispora omaniana, Septoglomus nakheelum and Rhizophagus arabicus spp. nov. D. omaniana and R. arabicus were isolated from the native, arid habitat, while S. nakheelum was isolated from a nearby irrigated date palm plantation. The discovery of three new species of AM fungi from this location suggests that a number of additional undescribed AM taxa may be present in such desert ecosystems. Further work to understand the diversity and functional significance of these new AM taxa may offer new opportunities for conservation, re-vegetation, and sustainable agriculture in extremely arid environments.

Projected climate change and rainfall variability will affect soil microbial communities, biogeochemical cycling and agriculture. Nitrogen (N) is the most limiting nutrient in agroecosystems and its cycling and availability is highly dependent on microbial driven processes. In agroecosystems, hydrolysis of organic nitrogen (N) is an important step in controlling soil N availability. We analyzed the effect of management (ecological intensive vs. conventional intensive) on N-cycling processes and involved microbial communities under climate change-induced rain regimes. Terrestrial model ecosystems originating from agroecosystems across Europe were subjected to four different rain regimes for 263 days. Using structural equation modelling we identified direct impacts of rain regimes on N-cycling processes, whereas N-related microbial communities were more resistant. In addition to rain regimes, management indirectly affected N-cycling processes via modifications of N-related microbial community composition. Ecological intensive management promoted a beneficial N-related microbial community composition involved in N-cycling processes under climate change-induced rain regimes. Exploratory analyses identified phosphorus-associated litter properties as possible drivers for the observed management effects on N-related microbial community composition. This work provides novel insights into mechanisms controlling agro-ecosystem functioning under climate change. As in many terrestrial ecosystems, nitrogen (N) is the most limiting nutrient for plant growth in agroecosystems 1-3. The last century has been characterized by a considerable increase of N inputs in agricultural soils 4-7 , mostly in mineral form (NH 4 +), making plant growth less dependent on microbial N provisioning. However, the increased amount of reactive N in the environment has severe environmental and human health consequences 7 .