1. Surface water connectivity can influence the richness and composition of fish assemblages, particularly in harsh environments where colonisation factors and access to seasonal refugia are required for species persistence. 2. Studies regarding influence of connectivity on Arctic fish distributions are limited and are rarely applied to whole assemblage patterns. To increase our understanding of how surface water connectivity and related hydrologic variables influence assemblage patterns, we investigated species richness and composition of Arctic lake fishes over a large region, 8500 km 2 , of the central Arctic Coastal Plain, Alaska. 3. We collected fish presence/non-detection data from 102 lakes and used a hierarchical multispecies occupancy framework to derive species richness and inform species composition patterns. Our mean estimate of regional richness was 12.3 (SD 0.5) species. Presence of a permanent channel connection was an overriding factor affecting species richness (mean 3.6, 95% CI 3.1-4.9), presumably driving lake colonisation potential. In lakes without a permanent channel connection, data suggest richness (mean 2.0, 95% CI 1.7-3.3) increased with the availability of in-lake winter refugia and with the potential of ephemeral connections during spring floods. 4. Fish species functional traits and environmental faunal filters contributed to patterns of richness and assemblage composition. Composition corresponded with richness in a coherent manner, where each successive level of richness contained several discrete assemblages that showed similar responses to the environment. Lakes with permanent channel connections contained both widespread and restricted species, while the species-poor lakes that lacked a connection contained mainly widespread species. 5. This work provides useful baseline information on the processes that drive the relations between patch connectivity and fish species richness and assemblage composition. The environmental processes that organise fish assemblages in Arctic lakes are likely to change in a warming climate.

4 4. In an analysis of five ecoregions around the Arctic, isolation, area, and topography were identified as strong drivers of gamma, alpha, and beta diversity. Physical isolation reduced 61 the gamma and alpha diversity, and changes in beta diversity between adjacent locations were due mainly to losses in species richness, rather than due to differences in species composition. Heterogeneity of habitats, environmental gradients, and geographic distance likely contributed to patterns of fish dissimilarity within and across ecoregions. 5. This study marks the first attempt to document large-scale patterns of freshwater fish biodiversity in the circumpolar Arctic. However, information gaps in space, time, and among taxonomic groups remain. Working towards inclusion of extensive old and new data will allow future studies to test for changes in the observed patterns of biodiversity. This is important given the potential impacts of climate change, land use, and biotic exchange on Arctic fish biodiversity in the future.

Migrating adult Pacific salmon (Oncorhynchus spp.) are sensitive to warm water (> 18 °C) with a range of consequences from decreased spawning success to early mortality. We examined the proportion of Yukon River Chinook salmon (O. tshawytscha) exhibiting evidence of heat stress to assess the potential that high temperatures contribute to freshwater adult mortality in a northern Pacific salmon population. Water temperatures greater than 18 °C have occurred almost annually in the Yukon River and correspond with low population abundance since the 1990s. Using gene transcription products and heat shock protein 70 biomarkers validated by field experiment we identified heat stress in half of Chinook salmon examined (54%, n = 477) across three main-stem locations and three tributaries in 2016–2017. Biomarkers tracked wide variation in water temperature (14–23 °C) within a tributary. The proportion of salmon with heat stress differed between years at four of the six locations, with more prevalent heat stress in the warmer year. This work demonstrates that warming water temperatures are currently affecting northern populations of Pacific Salmon.

Generalist feeding strategies are favoured in stressful or variable environments where flexibility in ecological traits is beneficial. Species that feed across multiple habitat types and trophic levels may impart stability on food webs through the use of readily available, alternative energy pools. In lakes, generalist fish species may take advantage of spatially and temporally variable prey by consuming both benthic and pelagic prey to meet their energy demands. Using stomach content and stable isotope analyses, we examined the feeding habits of fish species in Alaska's Arctic Coastal Plain (ACP) lakes to determine the prevalence of generalist feeding strategies as a mechanism for persistence in extreme environments (e.g. low productivity, extreme cold and short growing season). Generalist and flexible feeding strategies were evident in five common fish species. Fish fed on benthic and pelagic (or nektonic) prey and across trophic levels. Three species were clearly omnivorous, feeding on fish and their shared invertebrate prey. Dietary differences based on stomach content analysis often exceeded 70%, and overlap in dietary niches based on shared isotopic space varied from zero to 40%. Metrics of community-wide trophic structure varied with the number and identity of species involved and on the dietary overlap and niche size of individual fishes. Accumulation of energy from shared carbon sources by Arctic fishes creates redundancy in food webs, increasing likely resistance to perturbations or stochastic events. Therefore, the generalist and omnivorous feeding strategies employed by ACP fish may maintain energy flow and food web stability in extreme environments.

Despite their widespread presence in northern-latitude ecosystems, the ecological role of Ninespine Stickleback Pungitius pungitius is not well understood. Ninespine Stickleback can occupy both top and intermediate trophic levels in freshwater ecosystems, so their role in food webs as a predator on invertebrates and as a forage fish for upper level consumers probably is substantial. We introduced Ninespine Sticklebacks to fishless ponds to elucidate their potential effects as a predator on invertebrate communities in Arctic lentic freshwaters. We hypothesized that Ninespine Stickleback would affect freshwater invertebrate communities in a top-down manner. We predicted that the addition of Ninespine Sticklebacks to fishless ponds would: 1) reduce invertebrate taxonomic richness, 2) decrease overall invertebrate abundance, 3) reduce invertebrate biomass, and 4) decrease average invertebrate body size. We tested our hypothesis at 2 locations by adding Ninespine Stickleback to isolated ponds and compared invertebrate communities over time between fish-addition and fishless control ponds. Ninespine Sticklebacks exerted strong top-down pressure on invertebrate communities mainly by changing invertebrate taxonomic richness and biomass and, to a lesser extent, abundance and average invertebrate size. Our results supported the hypothesis that Ninespine Stickleback may help shape lentic food webs in the Arctic.

1. Climate change poses a significant threat to Arctic freshwater biodiversity, but impacts depend upon the strength of organism response to climate-related drivers. Currently, there is insufficient knowledge about Arctic freshwater biodiversity patterns to guide assessment, prediction, and management of biodiversity change.2. As part of the Circumpolar Biodiversity Monitoring Program's first freshwater assessment, we evaluated diversity of diatoms, benthic macroinvertebrates, and fish in North American Arctic rivers. Alpha diversity was assessed in relation to temperature, water chemistry, bedrock geology, and glaciation history to identify important environmental correlates. Biotic composition was compared among groups to evaluate response to environmental gradients.3. Macroinvertebrate α-diversity declined strongly with increasing latitude from 48°N to 82°N, whereas diatom and fish diversity peaked around 70°N without a clear latitudinal decline. Macroinvertebrate diversity was significantly positively related to air temperature. Diatom diversity was related to bedrock geology and temperature, whereas fish diversity was related to glaciation history. Fish and macroinvertebrate assemblages differed between sites in western Canada,where invertebrate composition was more variable, and Alaska, where fish composition was more variable. In sites with both diatom and macroinvertebrate data, diatom composition was distinct in Alaska, where richness was highest in former glacial refugia. Macroinvertebrate composition was distinct in lowest-latitude eastern and high-latitude western Canadian sites where temperature was highest. 5. Temperature, precipitation, geology, calcium, and substrate size were important environmental correlates for diatoms and macroinvertebrates, although the relative importance of each correlate differed. Diatom taxa were most strongly

No abstract

Thaw lakes are ubiquitous on arctic coastal plains (ACPs). While many thaw lakes have steep banks, stable water levels, and static surface areas, others only partially fill their basins and vary in area over the summer. These partially drained lakes (PDLs) are hydrologically connected to the wetlands immediately surrounding them. Heat and nutrient availability limit aquatic productivity on ACPs, and we hypothesized that shallow shorelines and greater hydrologic connectivity with the landscape should result in greater nutrient concentrations and biogeochemical cycling in PDLs. We tested this by monitoring water chemistry in lakes with varying levels of seasonal drainage in sandy and silty peaty lowland sites on the ACP of Alaska. One highly drained lake (N1) was significantly warmer than minimally drained lakes (minDLs) related to earlier ice off, reaching temperatures as high as 16 °C in June when minDLs still contained ice. Ammonia, total dissolved phosphorus, and dissolved organic carbon and nitrogen concentrations were higher in lakes with greater drainage, and concentrations in N1 rivaled those in the small, biologically productive ponds. Many PDLs displayed a midsummer decrease in nutrients consistent with assimilation by the aquatic ecosystem, and a late‐summer increase most likely related to runoff from drained lake margins following precipitation. N1 exported kilograms of ammonium and total dissolved phosphorus to the stream network over the summer. Given increased warming and drying in the arctic, the proportion of PDLs may be changing, which in turn may affect nutrient and organic matter availability in arctic lakes and export to downstream environments.