We report the complete molecular characterization of the DNA-A and DNA-B of a Brazilian tomato isolate of Tomato severe rugose virus (ToSRV) and the experimental host range of the virus determined using whitefly transmission tests. Genome analysis showed that ToSRV has a close evolutionary relationship with Tomato rugose mosaic virus. Of 33 plants species inoculated with viruliferous Bemisia tabaci biotype B, 13 species were susceptible to ToSRV, nine asymptomatically. Therefore, ToSRV disease management strategy should include the control of infected weeds close to tomato fields.
FUNGI ON SEEDS OF ORNAMENTALSABSTRACT -A study was made to detect the occurrence of fungi on seeds of nine species of herbaceous ornamental species (Dahlia pinnata, Petunia x hybrida, Phlox drummondii, Rudbeckia hirta, Salvia farinacea, S. splendens, Tagetes patula, Viola tricolor and Zinnia elegans) planted in the Federal District of Brazil. Subsamples of 100 seeds of each species, with or without desinfection with 70% alcohol and 1% sodium hypochlorite, were submitted to the blotter test on filter paper to detect fungi. Fungal species from these seeds were detected and isolated on PDA 32, with 88% of specimens identified as mitosporic fungi, 6% as Ascomycota, 3% as Zygomycota, and 3% as Oomycota. Alternaria, Cladosporium, Bipolaris, Curvularia, Exerohilum, Aspergillus and Penicillium were the most frequently detected genera. The highest number of fungi occurred
RESUMOA cafeicultura atual tem adotado novas tecnologias de condução e manejo da lavoura, como a irrigação e fertirrigação, visando aumentar a produtividade e, conseqüentemente, a margem de lucro. Com este trabalho, objetivou-se avaliar o efeito de lâminas de irrigação e parcelamentos da adubação, via fertirrigação, sob sistema de gotejamento no progresso da ferrugem e produtividade do cafeeiro. Instalou-se o ensaio na área experimental da Universidade Federal de Lavras, MG, com cafeeiros da cultivar Acaiá Cerrado MG-1474 (Coffea arabica L.) de seis anos de idade, em espaçamento de 0,6 x 3,0 m. O delineamento experimental foi em blocos casualizados com quatro repetições. Os tratamentos, em esquema de parcela subdividida, foram constituídos por cinco parcelas representando lâminas de irrigação correspondentes aos valores de 0%, 40%, 60%, 80% e 100% da evaporação do tanque Classe A (ECA), e três subparcelas com parcelamentos de adubação nitrogenada e potássica em 3, 6 e 9 vezes. Foram avaliadas a incidência e a severidade, a porcentagem de enfolhamento das plantas e a produtividade dos cafeeiros. Verificou-se que a severidade da ferrugem foi influenciada pela interação entre lâminas de irrigação e parcelamentos de adubação. Houve maior severidade da ferrugem no parcelamento em 3 e 9 vezes nas lâminas 0% e 60% ECA. Termos para indexação:Hemileia vastatrix, Coffea arabica, Irrigação, Epidemiologia. ABSTRACTThe coffee planting today has been adopting new technologies to manage and manipulate the farming, such as irrigation and fertirrigation, in order to increase productivity and consequently the profits. This work aimed at evaluating the effects of different irrigation depths and fertilizer splitting by fertirrigation under a drip irrigation system on the progress of the rust and productivity in coffee trees. The research was set at the experimental area of The Federal University of Lavras (UFLA), with six-year-old coffee trees cultivar Acaiá Cerrado MG-1474, (Coffea arabica L.) with distance between lines of 0,6 x 3,0 m. The experimental design was developed in randomized blocks with four repetitions. The treatments were formed by five levels of plots representing irrigation depths corresponding to the values of 0%, 40%, 60%, 80% and 100% of evaporation of the Class A Tank (ECA), and three subplots of nitrogen and potassium fertilizer splitting in 3, 6 and 9. This work evaluated the incidence and severity rate of rust in leaves, percentage of leaf number increasing and coffee plants productivity. It was verified that the severity of the rust was influenced by the interaction between irrigation depths and fertilizer splitting. There was more severity of the rust in the fertilizer splitting in 3 and 9 using irrigation depths 0% and 60% ECA.
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