Abstract.A new member of the phlebovirus genus, tentatively named Granada virus, was detected in sandflies collected in Spain. By showing the presence of specific neutralizing antibodies in human serum collected in Granada, we show that Granada virus infects humans. The analysis of the complete genome of Granada virus revealed that this agent is likely to be a natural reassortant of the recently described Massilia virus (donor of the long and short segments) with a yet unidentified phlebovirus (donor of the medium segment).

The administration of L. coryniformis K8 CECT5711 to an elderly population increased the immune response against the influenza vaccine and decreased symptoms associated with respiratory infections. Probiotic administration may be a natural and safe strategy to improve the efficacy of vaccines and to protect against common respiratory infections in susceptible populations.

We tested the capacity of the Sysmex UF-1000i system to detect yeasts in urine by screening a total of 22 132 urine samples received for culture in our microbiology laboratory during 1 year. We also analyzed different dilutions of previously filtered urine inoculated with a strain of Candida albicans. With clinical samples, a single cut-off point of 50 yeast-like cells (YLCs)/μL detected candiduria ≥10 000 colony forming units (CFU)/mL and >100 000 CFU/mL with a sensitivity of 87.3%/95.4%, a specificity of 97%, a negative predictive value of 95.9%, and a positive predictive value of 9.3%/5.7%. With the simulated samples, a linear relationship was observed between the dilution factor and the number of cells detected by UF-1000i. This instrument appears to be able to reliably rule out candiduria of a magnitude of at least 10 000 CFU/mL and facilitate urine sample screening, thereby providing fast results. The Sysmex UF1000i system can be adapted for candiduria screening by the use of an appropriate YLCs/μL cut-off point that takes account of the prevalence of candiduria in the population.

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The serum and urine proteins responsible for enhanced pigment production in Streptococcus agalactiae in culture media were purified by chromatography and were identified as amylases by comparison of their amino acid composition with that calculated for proteins with known sequences. Similar pigment-enhancing activity was displayed by other amylases of nonanimal origin and by maltooligosaccharides.

sions evident on both lower extremities were related to the presence antimycobacterial therapy points to an infectious process rather than a hypersensitivity reaction. A traumatic catheterization re-of leukocytoclastic vasculitis. Findings on examination of a bonemarrow aspirate and on chest radiographs and results of a urinalysis sulting in systemic BCG absorption, additional courses of BCG therapy notwithstanding intolerance symptoms, and delayed spewere normal. There was no proteinuria. Extensive investigations cific therapy may have furthered dissemination of BCG. revealed no signs of an underlying infectious or malignant disease, This case reaffirms the potential for major infectious complicaand disseminated infection with M. bovis was suspected. All cultures tions of bladder instillation of BCG in immunocompetent hosts of blood, bone marrow, sputum, and urine remained negative for [1]. BCG has proved to be more effective in the prophylaxis and Mycobacterium species. Histopathological evaluation of the bone treatment of superficial bladder tumors and carcinoma in situ than marrow and rectal ulcer revealed numerous nonnecrotizing granulomost chemotherapeutic agents [4]. A granulomatous reaction outmas with many epithelioid cells and giant cells. Special stains for side the urinary tract is rare, and pneumonitis or granulomatous mycobacteria, fungi, and bacteria were negative.hepatitis has been reported in only 0.9% of patients in large studies The patient received treatment with isoniazid (300 mg/d), ethambu-[1]. Recognition of risk factors, particularly traumatic catheterizatol (1,200 mg/d), and rifampin (600 mg/d). The patient's condition tion or concurrent cystitis, as well as the prompt treatment of improved within 1 week, and he was discharged. Purpura reappeared early side effects, should decrease the incidence of severe toxicity within 24 hours of exposure to cold. Purpuric lesions resolved within significantly. Our case illustrates that disseminated infection with 1 week and did not recur. When seen 3 and 6 months later, he was M. bovis after intravesical BCG instillation may be associated with completely well; findings on clinical examination and funduscopy cryoglobulinemia, monoclonal gammopathy, and an increase in and laboratory values were normal.the anticardiolipin antibody level. In our case, leukocytoclastic vasculitis was associated with cryoglobulinemia, monoclonal gammopathy, an elevated anti-J. M. Durand, C. Roubicek, F. Retornaz, E. Cretel, cardiolipin antibody level, and evidence of dissemination of BCG M. J. Payan, J. P. Bernard, G. Kaplanski, infection. The development of cryoglobulinemia has been deand J. Soubeyrand scribed during the course of different types of acute and chronic There have been only a few reports of cryoglobulinemia related to Mycobacterium tuberculosis infection [2, 3]. Cryoglobulinemia References has never been associated with M. bovis infection. Because we 1. Lamm DL, Van der Meijden APM, Morales A, et al. Incidence and treatment did not identify any chronic infla...

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