The osseo-integration, corrosion resistance, and tribological properties of the commonly used bioimplant alloy Ti-6Al-4V were enhanced using a laser-based surface nitridation process. The biomedical properties of the laser nitrided Ti-6Al-4V were investigated using experimental and computational methodologies. Electrochemical analysis of laser nitrided titanium in simulated body fluid (SBF) was performed to assess the biomedical characteristics in near-human body conditions. Additionally, the corrosive wear performance of these laser nitrided samples was evaluated using pin-on-disk geometry with a zirconia pin counter surface in SBF to mimic the biological scenario. Osteoblast studies were conducted to evaluate cell affinity towards titanium nitrided bioimplant material. Cells adhered to all substrates, with high viability. Initial cell adhesion was revealed by focal adhesion formation on all substrates. Cells can proliferate on samples treated with 1.89 and 2.12 × 10(6) J/m(2) laser conditions, while those treated with 1.70 × 10(6) J/m(2) inhibited proliferation. Thus, microstructural and phase observations, electrochemical analyses, corrosive wear evaluation, and cell behavior analysis of laser nitrided surface of bioimplant material (Ti-6Al-4V) indicated that laser nitriding greatly improves the performance of bioimplant material.
New peak detection
(NPD), as part of the LC–MS-based multi-attribute
method (MAM), allows for sensitive and unbiased detection of new or
changing site-specific attributes between a sample and reference that
is not possible with conventional UV or fluorescence detection-based
methods. MAM with NPD can serve as a purity test that can establish
whether a sample and the reference are similar. The broad implementation
of NPD in the biopharmaceutical industry has been limited by the potential
presence of false positives or artifacts, which increase the analysis
time and can trigger unnecessary investigations of product quality.
Our novel contributions to the success of NPD are the curation of
false positives, use of the known peak list concept, pairwise analysis
approach, and the development of a NPD system suitability control
strategy. In this report, we also introduce a unique experimental
design utilizing sequence variant co-mixes to measure NPD performance.
We show that NPD has superior performance relative to conventional
control system methods in the detection of an unexpected change as
compared with the reference. NPD is a new frontier in purity testing
that reduces subjectivity, need for analyst intervention, and potential
for missing unexpected product quality changes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.