Objective: To see the effects of Raj Nirwan Bati (RNB) on the hematobiochemical parameters, coagulation tests, and histopathological changes in the lungs, liver, kidneys and spleen and also to evaluate the immunomodulatory activity of RNB in Wistar rats. Methods: A total of 24 adult albino Wistar rats (of bodyweight 200-250 g) of either sex were divided into 3 groups. In the normal control group (n=8), no drug was administered and in the rest of the groups (A and B), RNB@ 26 mg/kg body weight./day and 260 mg/kg body weight/day respectively were administered orally for a period of 14 d. The blood samples were collected from the jugular vein at zero d (before drug administration) and after the 14th d of drug administration in both groups (A and B). The organ samples (lungs, liver, kidneys, and spleen) were collected after euthanizing the rats using Ketamine anesthesia overdose intraperitoneally (IP) after the 14th d of drug administration. White Blood Cells (WBC), Red Blood Cells (RBC), Hemoglobin (Hb), Hematocrit (HCT), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin(MCH), Mean Corpuscular Hemoglobin Concentration(MCHC), number of platelets, Differential Leucocyte Count(DLC) i.e. the percentage of neutrophils, lymphocytes, eosinophils, monocytes and basophils, neutrophil adhesion percentage, Prothrombin test (PT), Activated Partial Thromboplastin Time (APTT), fibrinogen, D-dimer, Lactate Dehydrogenase (LDH), urea, creatinine, Aspartate Amino Transferase (AST), Alanine amino Transferase (ALT), Alkaline Phosphatase (ALP), C-Reactive Protein (CRP) were evaluated and histological examination of organs were done. Results: After statistical analysis, it was found that the decrease in TLC, RBC, Hb, HCT, and LDH in Wistar rats after RNB intervention in Group A as compared to that of before RNB intervention, was found to be statistically significant (P=0.001, P=0.002, P=0.001, P=0.039, and P=0.008). On the other hand, an increase was observed in MCV, Urea, Creatinine and ALT values in the Wistar rats after RNB intervention in Group ‘A’ as compared to that of before RNB intervention and this increase in values was statistically significant (P=0.007, P=0.001, P<0.001 and P=0.038). After RNB intervention in Group B, the increase in MCH, fibrinogen concentration, and monocytes percentage, was found to be statistically significant (P=0.004, P=0.033, and P=0.001) as well as the decrease in PT and APTT was statistically significant (P=0.007and P=0.002). After comparing the Mean Hematobiochemical and coagulation test parameters in the rats of Group A and Group B, after RNB intervention, it was observed that the concentration of Urea, Creatinine, APTT, and D-dimer were less in Group B as compared to that of Group A and this difference was statistically significant (P<0.001, P<0.001, P<0.001 and P=0.022). Histologically the findings in the lungs of group B were more distortion of lung architecture, most of the alveoli become collapse and make emphysematous changes, more diffuse inflammatory infiltrate within interalveolar septa and around bronchioles as compared to Group A. In the liver of group B rats, the histological findings were mild to moderate distortion of lobular architecture, healthy hepatocytes with more activation of kupffer cells as well as larger and more aggregates of inflammatory cells as compared to group A. Histological findings of kidneys in group A and group B rats were similar to that of control group rats. Conclusion: The results suggest that the RNB is having an immunomodulatory effect. It might be helpful in the restoration of coagulation factors and can help treat the COVID patients. No harmful effects on the lungs, liver, kidney, and spleen were seen. These findings may act as baseline data for planning further clinical trials in human study subjects to evaluate the effects on various comorbidities.
Abhrak bhasma (AB) is a type of bhasma prepared from repeated incineration of mineral mica with decoctions of about 72 herbs. The particle size of Abhrak bhasm has been shown to be in the range of 29-88 nanometers and Fe, Ca, Si, Mg and K are found to be as major constituent. Many drugs developed to treat Central Nervous System (CNS) disorders are unable to reach the brain parenchyma in therapeutically relevant concentrations. The blood brain barrier protects brain parenchyma from the uctuation of plasma composition, from pathogenic agents and maintains homeostasis of the brain parenchyma by restricting non-specic ux of ions, peptides, proteins and even cells into and out the brain. Immunohistochemistry is being widely employed as a tool for biological studies. This study is conducted to examine the change in the continuity of Blood brain barrier by using immunohistochemistry, once Abhrak bhasm drug is given in experimental animal and also to examine the histology of organs. In this study a total of 30 adult albino Wistar rats of approximately 4 months age (approx. 150-200 gms) of either sex selected randomly to see the effect of Abhrak bhasm, an ayurvedic drug on Wistar rats. The rats were weighed, marked and divided into 5 groups each consisting of six animals. In normal control group (Group E), no drug was administered and in rest of the four treated groups (Group-A,B,C,D), Abhrak bhasm @ 36 mg/kg B.wt. was administered orally once in each rat. Brain, liver, kidneys,spleen and blood samples were collected in 10% formalin solution after euthanizing the rats at 0.5,2,6 & 12 hours of Abhrak bhasma drug intervention. The alterations in any of the biochemical parameters are within the tolerable limits of liver and kidney since the dose of abhrak bhasm did not affect liver and kidneys. In the present study, the increase in ALP level may be the result of alterations in metabolisms that occurred without any signicant alteration in histology of liver. After applying the immunohistochemistry with the research markers GFAP, CD 34, S 100, GLUT-1 and RECA-1 on the rats in groups A,B,C and D, there was no change in the intensity of immunohistochemistry, with respect to control. While on applying the Occludin, the intensity of immunohistochemistry was reduced in all the treatment groups as compared to the control group. On the basis of ndings of present study it can be concluded that the therapeutic dose of Abhrak bhasma causes changes at the level of tight junctions present in blood brain barrier in rats which is shown by immunohistochemistry with occludin research marker. There is no toxic effect of drug on different organs of rats as no signicant changes in histology of organs are seen. More studies need to be done to check the permeability of blood brain barrier for Abhrak bhasma drug, like calculating its concentration in brain tissues and other vital organs of rat.
The Ayurveda is the traditional system of medicine which is been used by Indian's. The science of life is the basic meaning of the word Ayurveda. The method of practicing had been a trusted and tested method in treating the illness. Abhrak Bhasma, an amorphous powder drug, is prepared by treating biotite (mica) with the juices of a number of reconstituent plants that make it a powerful cellular regenerator. It is commonly used ayurvedic drug against many diseases including hepatitis (hepatoprotective). It is also used as nervine tonic and is widely used in respiratory tract infections and megaloblastic anemia. It contains iron, magnesium, potassium, calcium and aluminium in trace amounts. It also contains silicates of iron, magnesium and aluminium.In this study a total of 30 adult albino Wistar rats of approximately 4 months age (approx. 150-200 gms) of either sex selected randomly to see the effect of Abhrak bhasm, an ayurvedic drug on hematological and biochemical prole of Wistar rats.The rats were weighed, marked and divided into 5 groups each consisting of six animals. In normal control group(Group E), no drug was administered and in rest of the four treated groups(Group-A,B,C,D), Abhrak bhasm @ 36 mg/kg B.wt. was administered orally once in each rat.All blood samples were collected from jugular veins by using 30 gauze needle in EDTA (Ethylene diamine tetraacetic acid) vials and in plain vials for hematological and biochemical investigations respectively at 0.5 hour,2 hours, 6 hours, 24 hours and at the end of study in Groups A, B, C, D and E respectively.A signicant increase in Neutrophils percentage and a signicant decrease in Lymphocytes percentage were observed in both group B and group C i.e. at 2 hours and 6 hours of drug administration respectively, as compared to control group. In two cases, in group B and group C i.e.at 2 hours and 6 hours of Abhrak bhasm drug administration, hypersegmented neutrophils were seen in blood smear. In three cases, two in group B and one in group D i.e. at 2 hours and 24 hours of drug administration, polychromasia were seen. The ndings in the present study indicate the erythropoeisis stimulant action and also a signicant phagocytic activity i.e. immunomodulatory activity of Sahastraputi Abhrak Bhasma drug. It consolidated the fact that Sahastraputi Abhrak Bhasma is highly regarded as Rasayan, thus leading to scope for exploring the mechanism of its activity through more animal and clinical studies.
Primary spindle cell rhabdomyosarcoma of the prostate is an uncommon variant of embryonal rhabdomyosarcoma seen in pediatric age group mostly during infancy and childhood. Rhabdomyosarcoma (RMS) arising from prostate predominantly presents obstructive urinary symptoms. To date, only one case of the spindle cell variant of embryonal rhabdomyosarcoma of the prostate have been described in literature. The delay in diagnosis and a more aggressive behavior of this malignancy contributed to an eventually unfavorable outcome. Early diagnosis and complete surgical resection offering patients for the best possibility of survival.
Rhabdomyosarcoma (RMS) in middle ear and mastoid are uncommon. Presentation of RMS in these sites include purulent or blood stained discharge, polypoidal mass and granulation tissue. Clinical diagnosis may be delayed due to similar presentation as Chronic Suppurative Otitis Media (CSOM). Authors herein report a case of six-year-old male patient, who presented with a blood stained discharge from right ear for 15 days. On otoscopic examination, a polypoidal mass in external auditory canal was identified. A history of ruptured right tympanic membrane with occasional discharge for two years had been given. Clinical diagnosis of CSOM with aural polyp had been made. Radiographically, it was a soft tissue lesion with poorly defined margins. There were no bony erosions with minimal collection in right middle ear cavity. On routine histopathological examination diagnosis of round cell tumour had been made. On Immunohistochemistry (IHC), tumour was immunoreactive for desmin and vimentin, while negative for Cytokeratin (CK), Leukocyte Common Antigen (LCA), chromogranin and synaptophysin. Tumour was diagnosed as RMS (Embryonal type). This case report shows importance of early diagnosis of RMS in middle ear, which is often delayed and misdiagnosed as CSOM. Delayed diagnosis may lead to, facial nerve involvement, local meningeal involvement and distant metastasis. Histopathological examination and IHC e.g., desmin positivity are the mainstay of diagnosis.
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