Context: The penetration of sealer into dentinal tubules is considered to be a desirable feature as sealers prevent the colonization of residual bacteria into the dentinal tubules because of their antibacterial properties. Aims: This study aims to compare the depth of penetration of ADSEAL, Sealapex, and BioRoot RCS into the dentinal tubules in the apical area using confocal laser scanning microscope (CLSM). Settings and Design: Quality improvement projects. Materials and Method: After decoronating 120 extracted single rooted teeth, preparation was done using ProTaper universal rotary files until F3. Then, the samples were divided into four groups (n = 40) and obturated by the following endodontic sealers: Group A-ADSEAL, Group B-Sealapex and Group C-BioRoot RCS along with F3 gutta-percha cone. Before root canal filling, the sealers were mixed with 0.1% Rhodamine B dye. All the samples were sectioned at 3 mm and 5 mm from apex and visualized under CLSM. Statistical Analysis Used: Data were analyzed using two-way ANOVA and Tukey post hoc tests. Results: BioRoot RCS has shown significantly higher depth of penetration than resin based sealer and calcium hydroxide based sealer (α < 0.05). Conclusion: At all root regions, BioRoot RCS exhibited more tubular penetration whereas ADSEAL exhibited less penetration.
Background: Bacteria and their by-products are responsible for various pulpal and periapical infections which can be classified as biofilm-mediated infections. Recently, nanoparticles have been introduced to decrease the bacterial load in endodontic infections. Aim and Objectives: The aim of the study was to compare and evaluate the antibacterial effect of silver nanoparticles alone and combination of silver nanoparticles with calcium hydroxide and chlorhexidine against Enterococcus faecalis . Materials and Methods: A pure culture of E. faecalis was used as the test microorganism. After 24 h of incubation the bacterial colonies were isolated and suspended in 5 ml of infusion broth followed by incubation at 37°C for 4 h. 0.5 McFarland of the bacterial suspension was prepared and then cultured on Mueller–Hinton agar culture medium with the help of a sterile swab. In each culture plate, five wells were created with a sterile pipette for placement of the samples. Results: Statistically significant difference in the antibacterial effect of the combination of silver nanocure gel with various medicaments was observed when compared to the effect of silver nanocure gel alone. Silver nanocure gel in combination with calcium hydroxide (Group B) was more efficacious as compared to silver nanocure gel alone (Group A) ( P < 0.001). Antibacterial activity of silver nanocure gel in combination with chlorhexidine (Group C) was also significantly higher than silver nanocure gel alone ( P < 0.001). Conclusion: The present study is an in vitro study, in which we concluded that the combination of all the intracanal medicaments is the best among for elimination of E. faecalis biofilm from the root canal. The above findings need to be tested in vivo also.
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