Sealer penetration in the dentinal tubules

Khullar, Sanjana; Aggarwal, Anurag; Chhina, Harleen; Kaur, Taranjit; Sharma, Mehak; Bala, Deepak

doi:10.4103/endo.endo_24_21

Cited by 10 publications

(10 citation statements)

References 16 publications

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“…The analysis of endodontic sealer penetration is often assessed in laboratory studies by sectioning filled extracted tooth perpendicular to the vertical axis of the root canal [ 24 , 25 , 26 ] and then evaluating the penetration of the sealer using scanning electron microscopy (SEM) or confocal laser scanning microscopy (CLSM). The latter provides detailed information about the distribution of sealers inside dentinal tubules at relatively low magnification using fluorescent-marked sealers [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Calcium Silicate-Based Sealer Dentinal Tubule Penetration—A Systematic Review of In Vitro Studies

et al. 2023

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The aim of this systematic review was to perform a qualitative synthesis of in vitro studies which evaluate and compare the penetration of calcium silicate-based sealers into dentinal tubules. A systematic advanced search was performed in Scopus, Embase, Medline (via PubMed), Web of Science, and Cochrane databases on the 1 December 2022. In vitro studies that compared the tubular penetration of at least two calcium silicate-based sealers in extracted human teeth were eligible. PRILE 2021 guidelines were used for the assessment of the risk of bias included studies. The search identified a total of 680 preliminary records, among which 12 studies were eligible for review. The most used methodology to evaluate sealer penetration was the use of a fluorochrome together with the tested sealers and the analysis of their penetration under confocal laser microscopy. Regarding the results of the included studies, calcium silicate-based sealers exhibit a favorable dentinal tubule penetration. Tubular penetration, however, can be affected by factors such as the irrigation protocol, sealer activation, the filling method used, and root canal morphology. EndoSequence BC Sealer showed the highest sealer penetration among the tested sealers. The influence of different fluorochromes on the results of dentinal tubule penetration studies should also be further explored. The in vitro nature of the included studies limits the applicability of the results into the clinical setting. Prospero registration: CRD42022383896

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Section: Introductionmentioning

confidence: 99%

Calcium Silicate-Based Sealer Dentinal Tubule Penetration—A Systematic Review of In Vitro Studies

et al. 2023

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“…34 The current study observed that the penetrability of all the sealers was increased at 5 mm than at 3 mm from the apex. Comparable findings were evaluated by studies done by Khullar et al 36 and Camilleri, 37 and Mc Micheal et al 38 stated that the dentinal tubules are comparatively fewer in number and also the size of tubules in the dentin reduces drastically as we go apically. Also, it is seen that the apical part of the radicular canal shows marked variations in the structures, such as, there might be irregularity in the direction and thickness of the dentinal tubules or there might be no tubule at all.…”

Section: Discussionmentioning

confidence: 82%

Comparative Evaluation of Penetrability of Various Bioceramic Sealers into the Tubules of Radicular Dentin

Choudhary,

Gupta,

Bharti

2024

Journal of Advanced Oral Research

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Aim: This study aimed to evaluate the penetration depth and persistence of sealer residues in obturated canals with three different bioceramic sealers using confocal microscopy. Materials and Methods: In total, 60 single-rooted human premolars were selected and decoronated to maintain the working length of 14 mm. The samples were biomechanically prepared and obturated with Protaper Universal GP coated with three different bioceramic sealers, that is, CeraSeal RC, Bio-C and MTA Fillapex. The specimens were divided into three groups ( n = 20) depending on the sealer used. Rhodamine B fluorescent dye was added to the sealers before using them for obturation. The obturated samples were sealed and placed in 100% humidity at 37oC for 7 days. Gutta-percha was removed from all the samples using retreatment files. Each sample was horizontally sectioned into 2 mm thick slices at 3 mm and 5 mm from the apex, which were analysed under a confocal scanning microscope to detect the depth of penetration of sealer into the dentinal tubules. Data were analysed using one-way ANOVA and Tukey’s post hoc tests. Results: Intergroup comparison of penetration depth at 3 mm showed that the mean depth of penetration among CeraSeal samples was found to be significantly more as compared to the other two materials, while, no statistically significant difference was found among Bio-C and MTA Fillapex with the significance level established at 5% ( P < .05). Similarly, an intergroup comparison of penetration depth at 5 mm showed that the mean depth of penetration among CeraSeal samples was found to be significantly more as compared to the other two materials, while, no statistically significant difference was found among Bio-C and MTA Fillapex. CeraSeal RC sealer showed significantly higher depth of penetration than Bio-C and MTA Fillapex. Conclusion: All bioceramic sealers show a significant penetration into the dentinal tubules. However, none of the sealers was completely removed from the tubules following retreatment.

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“…[18][19][20] Some studies still favor SEM for the interface analysis, while others have reported the advantages of CLSM. 18,19,21,24 The present study aimed to evaluate the effect of lentulospiral and ultrasonic activation on the intra-tubular penetration of the AH plus sealer by using confocal laser scanning microscopy. The irrigation protocol using 17% EDTA as a final rinse was to remove the smear layer and leave exposed collagen of the dentin to interact with the epoxy resin of sealers increasing the effectiveness of Mansoura Journal of Dentistry Mohamed et al…”

Section: Discussionmentioning

confidence: 99%

“…Dash et al 27 reported that Lentulo spiral technique had a mean penetration depth and percentage higher than the ultrasonic technique because the lentulo spiral forces the sealer along the instrument length, whereas ultrasonic files move the sealer centrifugally.The intratubular sealer penetration findings showed that the activated Irrisafe ultrasonic tip performed better than lentulo spiral in the apical region in agreement with previous studies. 24,28 These findings might result from the transmission of acoustic microstreaming energy generated by the ultrasonic tip, which probably forced the sealer into these areas. These effects frequently occur in the irrigants during passive ultrasonic irrigation.…”

Section: Discussionmentioning

confidence: 99%

Confocal Laser Scanning Microscopy Evaluation of the Effect of Lentulo Spiral and Ultrasonic Activation on Intra-tubular Sealer Penetration

2023

Mansoura Journal of Dentistry

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Objective: To evaluate the effect of lentulospiral and ultrasonic activation on the intra-tubular AH plus sealer penetration using confocal laser scanning microscopy. Materials and Methods: Twenty extracted single-rooted teeth were collected; teeth were prepared up to ProTaper finishing file F4 (size 40). Teeth were randomly divided into two main groups (n=10 teeth) according to activation techniques. The AH plus sealer with rhodamine B was activated by lentulospiral in group 1 and Irrisafe ultrasonic tip in group 2. The root canals were obturated with a single gutta-percha cone (size F4). The roots were sectioned at 3, 6, and 9 mm levels from the apex and examined with confocal laser scanning microscopy. Statistical analyses were performed at a 5% significance level. Results: At the cervical third, the lentulospiral group had significantly higher means of maximum depth (828.2±274.1 μm) and percentage (49.72±8.96 %) and area (2774.9±908.6 μm 2 ) of sealer penetration (P<0.05), while the Irrisafe group had significantly higher means of maximum depth (486.9±269.9 μm) and percentage (24.34±13.21 %) sealer penetration at the apical third (P<0.05). There was a positive correlation between the maximum depth (μm) and the area (μm2) of sealer penetration (P > 0.01 and confidence of 99 %). Conclusions: Regarding sealer activation, lentulospiral performed better in the cervical region while activated Irrisafe ultrasonic tip performed better in the apical region.

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Sealer penetration in the dentinal tubules

Cited by 10 publications

References 16 publications

Calcium Silicate-Based Sealer Dentinal Tubule Penetration—A Systematic Review of In Vitro Studies

Calcium Silicate-Based Sealer Dentinal Tubule Penetration—A Systematic Review of In Vitro Studies

Comparative Evaluation of Penetrability of Various Bioceramic Sealers into the Tubules of Radicular Dentin

Confocal Laser Scanning Microscopy Evaluation of the Effect of Lentulo Spiral and Ultrasonic Activation on Intra-tubular Sealer Penetration

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