In the year 1976, 9614 outpatient throat cultures were received in the Babies Hospital Bacteriology Laboratory. Cultures were plated on sheep blood a ar with bacitracin disc f applied and incubated overnight at 35 C. in a GASPAK anaerobic jar. 60% had no beta hemolytic colonies. Of the 40% with beta hemolytic colonies, 16% requircd further identification. 7% by replate, 9% by fluorescent antibody (PA). 21% of all cultures and 52% of all cultures with beta hemolytic colonies were presumptive Group A Strep (group 3). The incidence of PCP at the University of Florida during the 12 months period 10175 to 10176 was 6.8%(10/147 children treated with intensive chemotherapy). Primary diagnoses included: 7 leukemia and 3 solid tumor patients. 417 ALL patients were in remission(median duration 91.5 days); 3 leukemia and 2 solid tumor patients were in relapse.Presenting symptoms: fever-10110; cough-9/10; tachypnea-6/10 and dyspnea-3/10. In 9/10 cases an interstitial pneumonitis was revealed on x-ray and a lung biopsy was performed within 24 hours following this finding. In one child, first in our series, an abnormal x-ray was present 4 weeks before biopsy.The method of diagnosis, thoracoscopy, was performed under intram! ~cular anesthesia, without endotracheal tube. It allowed for direct observation and intrathoracic biopsy. Equipment utilized for this procedure was the Karl Stolls peritoneoscope set employing the Hopkins fibroscope and biopsy forceps. The method proved safe even in seriously ill patients and complications were minimal. 1 case of pneumothorax was resolved by reposition of tubing and there was 1 case of mild subcutaneous emphysema.All patients were treated with TPM 20mglkgld and SMX 100mglkgId 7 patients recovered, symptoms improved within 4 days after therapy was begun. 3 patients died, 2 patients had progressive tumors. 1 was complicated by sepsis. The cure rate in this incidence was 70%. Analysis of the data demonstrated a small false positive (F.P.) but a marked false negative (F.N.) rate for Bac-U-Dip (BUD). The false negative rate for Bacturcult (B/C) was zero and the false positive rate, when compared to a no-growth culture (NG) was also low (1170).The combination of Bacturcult and Bac-U-Dip provides both low false positive and false negative rates. Previous studies have shown that latex particles coated with glucose oxidaae (GO) would partially correct the metabolic defects in CGD polymorphonuclear leukocytes (Pm). UTILIZATION OF LIPOSOIBS FOR CORRECTION OF THE W T A - BOLIC DEFICIENCIES IN CHRONIC GRANULOMATOUS DISEASE (CCDIn order to develop a technique with better therapeutic advantage, we studied the efficacy of preparing liposomes (LP) containing the hydrogen peroxide (11202) generating ?a. The activity of GO incorporated within the LP could only be demonstrated after treatment of the LP with Triton X-100. LP were coated with heataggregated human IgC, thereby increasing their cs ability to be phagocytized hp PMN. CCD PMN oxidized glucose-l-f4C upon phagocytosis of GO-containing LP at levels 4-f...
A combination of trimethoprim and sulfamethoxazole was effective in the prevention and treatment of Pneumocystis carinii pneumonitis in cortisonetreated rats. Although all of 15 untreated rats died with P. carinii pneumonitis, none of 15 given trimethoprim-sulfamethoxazole prophylactically acquired the infection. After P. carinii pneumonitis was established, 9 of 14 rats recovered after treatment with trimethoprim-sulfamethoxazole compared with only 2 of 14 treated with pentamidine isethionate. Rifampin and clindamycin, separately or in combination with pentamidine, were ineffective in the prevention and treatment of P. carinii infection.
Live attenuated, cold-adapted (ca) influenza A vaccines administered intranasally have been well characterized as safe and immunogenic, but comparative data on protective efficacy are required for further development. In this study, 59 young children were divided into the following four groups based on prior exposure to influenza A (H3N2) virus: natural infection, live ca vaccine given intranasally, inactivated vaccine given im, and no previous exposure. Virus challenge with homologous live ca vaccine occurred 12 months after vaccination or natural infection. Prior natural infection and live ca vaccine significantly reduced ca virus shedding after challenge compared with inactivated vaccine or no prior exposure to influenza A virus. Prechallenge nasal IgA, detected almost exclusively in subjects naturally infected or vaccinated with live ca virus, was associated with protection. Although inactivated vaccine failed to produce significant local IgA during the primary response, it seemed to prime for secondary local antibody responses after challenge with live ca virus.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.