Our view refers to clarify the seroprevalence of brucellosis infection in dairy cows in Menoufiya Governorate and to evaluate the hematological, biochemical and immunological parameters changes in blood of infected cows compared to healthy group. Blood samples of 100 dairy cows (3-5) years from private farm and Menoufiya abattoir were screened for Brucella infection using (BAPAT) test and groups, the first group consistent of ( 25) samples which serologically positive to brucella and the second group consistent of (75) samples which brucella negative. Hematological analysis revealed normocytic anemia and Lymphopenia. Biochemical analysis of brucella positive serum infected cows when compared with negative control revealed significant elevation in (AST), (ALT), (ALP) and (GGT) activity in addition to non-significant increases in creatinine level, however a significant decrease in serum urea in diseased cows was recorded. Total protein, α1 globulin, β2 globulin revealed significant decrease while non-significant change was observed in α2, β1 and γ globulin in infected group. Immunologically IL-1 β and IL-10 showed significant elevation in infected group when compared with negative control group. Brucella infectionBuffered acidified plate agglutination test.

A total of 75 samples of serum, muscles, lungs, livers and kidneys of slaughtered camels (15 of each) were collected from Banha abattoir in Kalubia Governorate and they were analyzed for the presence of heavy metals (Lead, Cadmium, Copper and Zinc) by using Atomic Absorption Spectrophotometer. The obtained results indicated that the mean values of Lead concentrations in the examined samples of serum, muscle, lung, liver and kidney were 0.014 μg/ml, 0.107, 0.81, 0.66 and 0.57 mg/kg wet weight respectively. Results showed that mean concentrations of lead in lungs and livers were significantly higher (p≤0.05) than that reported in kidneys and muscles. However, the mean values of Cadmium concentrations in the examined samples of serum, muscle, lung, liver and kidney were 0.007 μg/ml, 0.07, 0.39, 0.52 and 1.28 mg/kg wet weight respectively. Significant differences (p≤0.05) in the mean concentrations of cadmium were recorded between the studied tissues as the highest mean concentration was reported in the kidneys, then in the livers, while the muscles contained the minimum mean concentration. Meanwhile, the mean values of Copper concentrations in the examined samples of serum, muscle, lung, liver and kidney were 1.29 μg/ml, 1.37, 2.75, 3.94 and 3.25 mg/kg wet weight respectively. Significant differences (p≤0.05) in the mean concentrations of Copper were recorded between the studied tissues as the highest mean concentration was reported in the liver. Whereas, the mean values of Zinc concentrations in the examined samples of serum , muscle, lung, liver and kidney were 1.96 μg /ml, 16.94, 10.68, 22.22 and 8.43 mg/kg wet weight respectively. Significant differences (p≤0.05) in the mean concentrations of Zinc were recorded between the studied tissues as the highest mean concentration was reported in the liver and muscles. All examined heavy metals were within the normal level in serum. Generally, lungs, livers and kidneys were found to have the highest significant levels of Pb and Cd and muscles the lowest levels.

Fifty buffaloes(healthy and mastitic udders) were milked aseptically from separate regions of Kaliobia governorate .California mastitis test (CMT) and bacteriological examination were achieved .Upon susceptibility testing (12) antibiotics were used to explain different susceptibility patterns of the isolated bacteriaThe objective of this study was the detection of oxytetracycline, sulphadimidine, penicillin G and ampicillin residues in raw buffalo's milk. 45 samples of raw milk ( 13 for oxytetracycline ,11 for sulphadimidine , 11 for penicillin G and 10 for ampicillin) were collected from private farms in Banha at Kaliobia Governorate. The period of exiperiment was January-March 2015 and the sample was analyzed with high performance liquid chromatography (HPLC) method . Out of the samples examined for oxytetracycline 30.76% ( 4 /13) were found to contain oxytetracycline residues and 54.5% (6/11) were found to contain sulfonamide residue . The amount of oxytetracycline in positive milk samples were found 452 ng/ml, 560 ng/ml, 1475 ng/ml and 2833 ng/ml which are much higher than WHO and FD recommended level. While, sulphadimidine residue was detected in the range of 3-22 ng/ml in 2 samples and 28 -44 ng/ml in 4 samples which were lower than WHO and FD recommended level. Penicillin G and ampicillin residues did not found in the examined milk samples. This study indicates the presence of oxytetracycline residues more than allowed amount. Regulatory authorities should ensure proper withdrawal period before milking the animals and definite supervisions are necessary on application of these drugs.