were no major biogeographic differences in the types of Diptera that were used in 3 particular regions, though some subtle differences were apparent. Likewise there were no differences between the two major clades of Ceropegia, but clear differences when comparing the range of Diptera exploited by Ceropegia with that of the stapeliads. This clade, one of the largest in the Asclepiadoideae, is a fascinating example of a species radiation driven by an apparently relatively uniform set of pollinators.
The evolutionary potential of populations inhabiting marginal areas has been extensively debated and directly affects their conservation value. Gene flow is one of the main factors influencing selection, adaptive potential and thus, local adaptation processes in marginal areas. The effects of differential gene flow provenance are still not well understood, since studies on gene flow between marginal populations have been underrepresented in the literature. This kind of gene flow can be especially beneficial because it can provide both adaptive allelic combinations originated under similar environmental conditions and genetic variation on which selection can act.
We conducted a study on the effects of different gene flow provenance on marginal populations of Mediterranean alpine Silene ciliata Pourret (Caryophyllaceae) replicated in three mountain ranges of Central Spain. The delineation of optimal and marginal areas of the species distribution was based on environmental differentiation and relied on the ecological definition of centrality and marginality. We experimentally tested the effect of three different types of pollen‐mediated gene flow on germination rate, seedling size and survival rate in marginal populations and assessed their effects by establishing in situ common gardens. To further assess the evolutionary potential of marginal populations, we performed a reciprocal sowing experiment and measured the same fitness components to determine the extent of local adaptation.
We found that gene flow between marginal populations improved germination rate and seedling survival with regard to gene flow from optimal to marginal populations and within marginal populations. In reciprocal sowing experiments, seedling survival rate was higher when the seed source was from marginal areas than when it was from optimal areas in both marginal and optimal sowing sites.
Synthesis. Our results suggest that gene flow between marginal populations from similar environmental conditions increases the fitness of the recipient population by increasing genetic diversity and simultaneously providing adaptive alleles generated under similar selective pressures. Results also highlight the adaptive potential of marginal populations as genetic diversity from marginal areas may provide a fitness advantage to the populations in optimal areas. In this context, the adaptive value of marginal populations increases their relevance and potential use in conservation management.
The composition of flower scent and the timing of emission are crucial for chemical communication between plants and their pollinators; hence, they are key traits for the characterization of pollination syndromes. In many plants, however, plants are assigned to a syndrome based on inexpensive to measure flower traits, such as color, time of flower opening, and shape. We compared day and night scents from 31 Sileneae species and tested for quantitative and semi-quantitative differences in scent among species classified a priori as diurnal or nocturnal. As most Sileneae species are not only visited by either diurnal or nocturnal animals as predicted by their syndrome, we hypothesized that, even if flower scent were preferentially emitted during the day or at night, most species also would emit some scents during the opposing periods of the day. This phenomenon would contribute to the generalized assemblage of flower visitors usually observed in Sileneae species. We found that diel variations of scent often were not congruent with the syndrome definition, but could partially be explained by taxonomy and sampling times. Most species emitted compounds with attractive potential to insects during both the night and day. Our results highlight the current opinion that syndromes are not watertight compartments evolved to exclude some flower visitors. Thus, important information may be lost when scents are collected either during day- or night-time, depending on the a priori classification of the species as diurnal or nocturnal.
Flower scents are complex blends of volatile compounds often shaped by selection pressures exerted by mutualistic and antagonistic interaction partners, but also by phylogenetic constraints. So far, little is known about the relative effect of selection and phylogenetic signal on scent patterns, and no study to date analyzed the phylogenetic signal in multivariate semiquantitative scent patterns. We analyzed the phylogenetic signal in qualitative and semi-quantitative patterns of flower scents in 47 Sileneae (Caryophyllaceae) species using phylogenetic principal component analysis (pPCA) and several indices of univariate and multivariate phylogenetic signal. As previous results showed that Sileneae species are visited by diurnal and nocturnal pollinators and flower scents also vary along the day, we compared the phylogenetic signal between night and day. Multivariate pPCA analyses identified compounds that correlate with the phylogeny at both night and day; however, multivariate Bloomberg 0 s K detected phylogenetic signal in the dataset of night scents, but not of day scents. In multivariate qualitative datasets, phylogenetic signal was neither found for day nor for night scents. In univariate analyses, phylogenetic signal was detected for some compounds both for day and night scents. Overall, we found that the phylogenetic signal is stronger in night compared to day scents, which might be owed to the different guilds of pollinators at day and night. At day, the phylogenetic resemblance of Sileneae scents might be masked by or disappear due to divergent selective pressures exerted by a diverse guild of pollinators on the different species. In contrast, we hypothesize that the nocturnal moth pollinators exert similar selective pressures; thus, the phylogenetic similarity of scent profiles might be conserved. Future studies of scent phylogenetic signal must consider not only the usage of qualitative measures but also semiquantitative analyses.
The circadian rhythm regulating floral attractiveness and reward in S. colorata is predominantly adapted to nocturnal flower visitors. However, favourable environmental conditions lengthen the optimal daily period of flower attraction and pollination towards morning. This allows the complementarity of day and night pollination. Diurnal pollination may help to compensate the plant reproductive success when nocturnal pollinators are scarce and when the net outcome of H. sancta shifts from mutualism to parasitism. These results suggest a functional mechanism explaining why the supposed nocturnal syndrome of many Silene species does not successfully predict their pollinator guilds.
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