The SARS-CoV-2 causes severe pulmonary infectious disease with an exponential spread-ability. In the present research, we have tried to look into the molecular cause of disease, dealing with the development and spread of the coronavirus disease 2019 (COVID-19). Therefore, different approaches have investigated against disease development and infection in this research; First, We identified hsa-miR-1307-3p out of 1872 pooled microRNAs, as the best miRNA, with the highest affinity to SARS-CoV-2 genome and its related cell signaling pathways. Second, the findings presented that this miRNA had a considerable role in PI3K/Act, endocytosis, and type 2 diabetes, moreover, it may play a critical role in the prevention of GRP78 production and the virus entering, proliferation and development. Third, nearly 1033 medicinal herbal compounds were collected and docked with ACE2, TMPRSS2, GRP78, and AT1R receptors, which were the most noticeable receptors in causing the COVID-19. Among them, there were three common compounds including berbamine, hypericin, and hesperidin, which were more effective and appropriate to prevent the COVID-19 infection. Also, it was revealed some of these chemical compounds which had a greater affinity for AT1R receptor inhibitors can be suitable therapeutic targets for inhibiting AT1R and preventing the adverse side effects of this receptor. According to the result, clinical assessment of these three herbal compounds and hsa-miR-1307-3p may have significant outcomes for the prevention, control, and treatment of COVID-19 infection.

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Background: The cause of COVID-19 global pandemic is SARS-CoV-2. Given the outbreak of this disease, it is so important to find a treatment. One strategy to cope with COVID-19 is to use the active ingredients of medicinal plants. In this study, the effect of active substances was surveyed in inhibiting four important druggable targets, including S protein, 3CLpro, RdRp, and N protein. RdRp controls the replication of SARS-CoV-2 and is crucial for its life cycle. 3CLpro is the main protease of the virus and could be another therapeutic target. Moreover, N protein and S protein are responsible for SARS-CoV-2 assembly and attaching, respectively.   Methods: The 3D structures of herbal active ingredients were prepared and docked with the mentioned SARS-CoV-2 proteins to obtain their affinity. Then, available antiviral drugs introduced in other investigations were docked using similar tools and compared with the results of this study. Finally, other properties of natural compounds were uncovered for drug designing.   Results: The outcomes of the study revealed that Linarin, Amentoflavone, (-)-Catechin Gallate and Hypericin from Chrysanthemum morifolium, Hypericum perforatum, Humulus lupulus, and Hibiscus sabdariffa had the highest affinity for these basic proteins and in some cases, their affinity was much higher than antiviral medicines.   Conclusion: In addition to having high affinity, these herb active ingredients have antioxidant, vasoprotective, anticarcinogenic, and antiviral properties. Therefore, they can be used as extremely safe therapeutic compounds in drug design studies to control COVID-19.

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Aim:Prostate cancer is one of the most frequent cancers in men and it is reported that about one in nine men experience the disease in their lifetime. It has been found that in some cases, HPV can be a culprit for prostate cancer. The present meta-analysis study aims to examine whether human papillomavirus (HPV) serves as a foundation of human prostate cancer.Methods:Related studies to HPV and prostate cancer from 1990 to 2020 were sought in PubMed and Google Scholar. Earlier published expert reviews and systematic meta-analysis were utilized as a supplementary source to recognize appropriate articles. Finally, data from 54 articles, with 7072 prostate cancer cases, were pooled and analyzed.Results:The pooled rating for the odds ratio was 17.42%. Stratified pooled analyses were subsequently performed according to the HPV detection of geographical regions, publication years, the number of prostate cases, and HPV-positive cases.Conclusion:The meta-analysis confirmed the variant frequencies of HPV positivity in prostate cancer of different geographic zones. It has been found that there can be a potential link between HPV and prostate cancer. Yet, further investigations of this cancer relating to HPV may be necessary.

Strategies the outcome for AML patients involve the use of new agents with reduced toxicity. Gemtuzumab ozogamicin (GO) is an immunoconjugate that has shown controversial activity in AML. It is composed of a humanized anti-CD33 antibody linked to the potent antitumor antibiotic calicheamicin. This agent provides a novel method of drug delivery by using the monoclonal antibody to target CD33+ leukemic cells without many of the systemic toxicities associated with traditional chemotherapeutic agents. The therapeutic activity and toxicity profile of GO were assessed in 30 patients from our institution (12 males, 18 females), aged 18 to 74 (median, 63 years), with AML who were either in first (n = 5), second (n = 10), third (n = 12), or fourth line (n = 3) of therapy. The drug was administered either at the dose of 6 mg/m2 as a single 2-hour IV infusion on days 1 and 15 (n = 6) or at the dose of 3 mg/m2 on days 1, 4 and 7 (n = 24). The overall CR/CRp proportion after GO was 30% (95% CI, 15% – 49%). The CR/CRp proportions were 20%, 30%, and 33% for patients treated in first, second, or further therapeutic line respectively. There was no influence of cytogenetics, antecedents of myelodysplasia, or previous treatment by stem cell transplantation on CR/CRp proportions. Only age (54% in patients < 60 years vs 12% for those ≥ 60 years; p = 0.01) and surprisingly gender (50% in females vs none in males; p = 0.003) influenced CR/CRp proportions. Of the 30 patients, 20 (67%) received the scheduled injections. Reasons for not administering the full dose were progressive disease and drug-induced toxicity. Myelosuppression was universally observed during treatment (93%). Additional severe toxicities that occurred during therapy were: febrile neutropenia and infections (63%), intolerance (10%), liver toxicity (10%). Veno-occlusive disease was observed in one patient. Salvage or consolidation treatment by chemotherapy or donor lymphocyte infusion (in previously transplanted patients) was administered to 16 patients only. CR/CRp proportion raises to 43% after salvage therapy. The duration of previous CR appeared then as of prognostic value for CR achievement (17% for previous CR < 6 months vs 64% for CR ≥ 6 months; p = 0.02). Three patients received allogeneic stem cell transplantation as postremission therapy. Eight of the 13 patients who achieved CR relapsed (median, 4.1 months). After relapse, 3 patients were retreated with GO, of which 2 achieved CR again. The median DFS time was 3 months with a 6–month DFS at 22%. The median overall survival time for all patients was 6.8 months with a 6-month and a 1-year survival rates of 57% and 35% respectively. Age (p = 0.01), duration of previous CR (p = 0.002), and achievement of CR after GO therapy (p = 0.01) were the only factors that impact on outcome in terms of OS. These results suggest that the option of single agent GO therapy is viable in this patient population. Although only observed in 30%, responses were present regardless of the karyotype. However, responses were short warranting additional studies using GO in combination with other drugs or chemotherapy.