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Background: Immunohistochemistry is a key tool for the analysis of localization of target molecules within tissues. It has a significant role in the identification of tumors lacking evidence of lineage differentiation on the basis of routine light microscopic morphology alone. Approximately 90% of tumors posing diagnostic difficulties by morphology could be accurately classified by exploiting immunohistochemistry. The aim of this study is to identify the true identity of malignant small round cell tumors by immunohistochemical analysis. Materials and Methods:This was a retrospective study done in Department of Histopathology of B.P.Koirala Memorial Cancer Hospital from January 2010 to April 2011.A total of 40 cases small round cell tumors were selected for immunostaining. The immunohistochemistry technique used is the Polymer detection-EnvisionTM System, a two step staining technique based on Horse Radish Peroxidase labeled dextran polymer technology (DAKO Company). Results: Out of 40 cases of malignant small round cell tumors, there were 21 cases (52.5%) of Non- Hodgkin Lymphoma , 11 cases (27.5%) of Ewing’s Sarcoma/Primitive Neuroectodermal Tumor, 1 case (2.5%) of Lymphoblastic Lymphoma , 1 case (2.5%) of Rhabdomyosarcoma, 2 cases (5%) of Low grade neuroendocrine tumor, 1 case (2.5%) of Neuroblastoma, 2 cases (5%) of Poorly differentiated Synovial Sarcoma (small cell variant), 1case (2.5%) of Malignant Melanoma (small cell variant). Conclusion: Immunohistochemistry is a valuable adjunct to routine hematoxylin and eosin staining for adequate and accurate categorization of malignant small round cell tumors. Keywords: Immunohistochemistry; Malignant Small Round Cell Tumor; Non Hodgkin’s Lymphoma; Ewing’s Sarcoma; Rhabdomyosarcoma DOI: http://dx.doi.org/10.3126/jpn.v1i2.5398 JPN 2011; 1(2): 87-91

Background: The incidence of esophageal adenocarcinoma is increasing in trends. Squamous cell carcinoma is associated with tobacco and alcohol consumption. Adenocarcinoma is often associated with a history of gastroesophageal reflux disease and Barrett's esophagus. The aim of this study was to find out the histological pattern of esophageal cancer in one of the largest Cancer center in Nepal.Materials and Methods: Between January 2008 and December 2011, a total of 106 cases of esophageal cancer were received in the department of pathology, BP Koirala Memorial Cancer Hospital. Relevant clinical data were retrieved from computer database of the hospital.Results: A total of 106 cases of esophageal carcinomas were diagnosed during a three years period. There were 68 (64.15%) cases of squamous cell carcinoma, 33 (31.13%) cases of adenocarcinoma including signet ring cell carcinoma, 4 (3.76%) cases of undifferentiated carcinoma and 1 (0.94%) case of small cell carcinoma. The esophageal cancer was most common in the age group of 61-70 years of age. Distal third of esophagus was the most common site for esophageal carcinoma, followed by middle esophagus and proximal esophagus.Conclusion: The most frequent type of esophageal carcinoma is squamous cell carcinoma followed by adenocarcinoma. Distal esophagus is the most common site with male preponderance.Journal of Pathology of Nepal (2012) Vol. 2, 277-281DOI: http://dx.doi.org/10.3126/jpn.v2i4.6877

Background: Maternal mortality is associated with the unmet need of family planning. Family planning has been subjected as taboos in the Muslim community with low use of its methods. Gulariya Municipality is the second largest community for Muslim. This research focuses on knowledge, attitude and practice of family planning among Muslim women of reproductive age.Methods: Descriptive cross-sectional study design was undertaken in Gulariya Municipality. Married Muslim women of reproductive age group from 164 households were interviewed using systematic random sampling. Key informant interview was also applied.Results: The percentage of women who have knowledge on family planning methods was found 94.5%. On the means of contraception, most of the women (73.2%) knew about Depo. The attitude of the respondents was seen positive. A total of 79.3% of women were found to have practiced temporary means of family planning while none of the women surveyed were found using permanent family planning methods. Association between all socio-economic and demographic factors with knowledge of family planning methods were found insignificant at 0.05 level of significance. However, association between wealth rank and practices on family planning shows statistically significant association. Conclusions: Majority of women have knowledge on family planning, but still lack knowledge on few method like condom. Most of them found practicing family planning, despite the practice was not seen being done regularly. Language barrier seems to be influencing knowledge, attitude and practice of family planning. The research warrants newer strategies to be developed and employed to deal with the multi-disciplinary prospective of family planning among Muslim women.Keywords: Family planning; knowledge, attitude and practice; muslim women

Background: Breast carcinoma is the most common malignancy diagnosed among women worldwide and second leading cause of cancer mortality. One of the hallmarks of the disease is expression of estrogen receptor and progesterone receptor that ultimately drives prognosis and treatment modalities of the patient. The objective of this study was to determine the Estrogen and Progesterone receptor status in relation to histological grade of tumor. Materials and Methods:This was an observational study, carried out in the department of Pathology, BP Koirala Memorial cancer Hospital, Bharatpur, from January 2010 to December 2010. A total of 136 patients with histological proven diagnosis of breast carcinoma was included in this study. These cases were graded according to the modified Bloom and Richardson criteria into three histological grades. We used immunohistochemistry to evaluate the expression of Estrogen and Progesterone in relation to histological grade of tumor.Results: Out of 136 cases, there were 131 (96%) cases of infiltrating ductal carcinoma with mean age of 48 years. Majority of cases were grade II (59%) followed by grade III (21%) and grade I (20%). Estrogen Receptor and Progesterone Receptor expression were seen in 28% and 19% respectively. In grade I, 16 (59%) and 10 (37%) cases out of 27 were Estrogen Receptor and Progesterone Receptor positive respectively. In grade II, 21 (26%) and 15(19%) out of 80 cases were Estrogen Receptor and Progesterone Receptor positive respectively. In grade III, 1(3%) and 1 (3%) cases were positive for Estrogen Receptor and Progesterone Receptor respectively. Conclusions

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INTRODUCTION: Drug resistant Salmonella spp. is endemic in several Asian countries. Nalidixic acid-resistant Salmonella enterica serovar Typhi and Salmonella enterica serovar Paratyphi A show reduced susceptibility to fluoroquinolones and have resulted in a rise in treatment failures. Over the past few decades, nalidixic acid-resistant Salmonella spp have emerged in Nepal as well.MATERIAL & METHODS: This is a retrospective study that aims to provide a more recent antibiogram of S. Typhi and S. Paratyphi A isolates in Kathmandu. Between Poush, 2071 and Ashwin, 2072 (December 16, 2014 to October 17, 2015), 186 culture positive cases of enteric fever were diagnosed at the Civil Service Hospital. Upon isolation of S. Typhi or S. Paratyphi A, antimicrobial susceptibility testing was performed with amoxicillin, azithromycin, ceftriaxone, chloramphenicol, ciprofloxacin, cotrimoxazole, nalidixic acid, ofloxacin and tetracyciline.RESULTS: This study shows a much higher frequency of nalidixic acid-resistance in Kathmandu than previously reported; 95.7% in Salmonella enterica serovar Paratyphi A and 86.5% in Salmonella enterica serovar Typhi.The rates of ciprofloxacinand ofloxacin-resistance were over 50% in both serovar.CONCLUSION: In Nepal, it is necessary to reevaluate the use of fluoroquinolone therapy and introduce feasible alternatives so as to curb treatment failures.Journal of Universal College of Medical Sciences (2017) Vol. 5, No, 2, Page: 22-25

Microcystic meningioma is a distinct morphological variant of meningioma, characterized by loose texture and microcysts with formation of large extracellular spaces containing edematous fluid. The tumor cells have stellate and vacuolated cytoplasm with long cytoplasmic processes. We report a case of microcystic meningioma occurring in a 60 year-old man. Histological features showed tumor cells with abundant foamy cytoplasm, pleomorphic nucleus with vesicular chromatin and prominent nucleoli which mimicked pleomorphic xanthoastrocytoma. The clinical presentation and histopathological diagnostic dilemma along with immunohistochemistry is discussed. Keywords: MicrocysticMeningioma; Pleomorphic xanthoastrocytoma Immunohistochemistry DOI: http://dx.doi.org/10.3126/jpn.v1i2.5413 JPN 2011; 1(2): 158-160