Helichrysum gymnocephalum essential oil (EO) was prepared by hydrodistillation of its leaves and characterized by GC-MS and quantified by GC-FID. Twenty three compounds were identified. 1,8-Cineole (47.4%), bicyclosesquiphellandrene (5.6%), γ-curcumene (5.6%), α-amorphene (5.1%) and bicyclogermacrene (5%) were the main components. Our results confirmed the important chemical variability of H. gymnocephalum. The essential oil was tested in vitro for cytotoxic (on human breast cancer cells MCF-7), antimalarial (Plasmodium falciparum: FcB1-Columbia strain, chloroquine-resistant) and antioxidant (ABTS and DPPH assays) activities. H. gymnocephalum EO was found to be active against MCF-7 cells, with an IC50 of 16 ± 2 mg/L. The essential oil was active against P. falciparum (IC50 = 25 ± 1 mg/L). However, the essential oil exhibited a poor antioxidant activity in the DPPH (IC50 value > 1,000 mg/L) and ABTS (IC50 value = 1,487.67 ± 47.70 mg/L) assays. We have reviewed the existing results on the anticancer activity of essential oils on MCF-7 cell line and on their antiplasmodial activity against the P. falciparum. The aim was to establish correlations between the identified compounds and their biological activities (antiplasmodial and anticancer). β-Selinene (R² = 0.76), α-terpinolene (R² = 0.88) and aromadendrene (R² = 0.90) presented a higher relationship with the anti-cancer activity. However, only calamenene (R² = 0.70) showed a significant correlation for the antiplasmodial activity.
Cereal Chem. 85(6):799-807Thioredoxin is one of the key systems controlling cellular redox balance in all living organisms. Plant thioredoxins are a diverse multigene family divided into two systems, the chloroplastic and the cytoplasmic systems, which are distinguishable by the electron donor and by the enzyme that catalyses thioredoxin reduction. In cereal seed, the thioredoxin (Trx) h system acts in the developing phase, controlling the delivery of compounds during seed filling. Early in the development of the imbibed seed, it promotes the mobilization of storage nitrogen and carbon in the endosperm by inhibiting the inactivators of amylolytic enzyme and by activating a specific serine protease, thiocalsin. During seed maturation and germination, the Trx h system controls oxidative stress in the living tissues, specifically in the scutellum and the aleurone layer, where it accumulates in the nucleus. The overexpression and the suppression of Trx confirm these features and constitute a powerful tool to manage seed quality, due to the large number of Trx h isoforms and to the specific nature of some of them.
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