A total number of 350 random samples of raw milk, pasteurized milk, dairy products, tap water and water of udder wash were collected from markets in port-Said Province and examined for prevalence of Aeromonas species using starch ampicillin medium, The incidence of Aeromonas species isolated from raw milk, pasteurized milk, kariesh cheese, dmietta cheese, refrigerated white cheese, tap water and water of udder wash were 58%, 26%, 70%, 48%, 40%, 16% and 68%, respectively. The total number of mesophilic Aeromonas isolates from 350 examined samples was 202 isolates of which 104 (51.5%) isolates were A. hydrophila, 60 (29.7%) isolates were A. caviae, 25 (12.4%) isolates were A. sobria and 11 (5.4%) isolates were A. schubertii. The highest number of isolates (42) were recovered from water of udder wash samples, while the lowest number of isolates were recovered from pasteurized milk and tap water samples. Result of antibiotic sensitivity test revealed high degree of sensitivity towards chloramphenicol, amikacin, ceftriaxone and ciprofloxacin, resistant to ampicillin and colistin sulphate. Result of agarose gel electrophoresis revealed that A. hydrophila , A. caviae and A. schubertii have only one plasmid DNA with molecular weight 2.8 kbp While A. sobria was negative for plasmid. hydrophila micro-organism isolated from raw milk and milk product. SC.V.M.J, Fac. Vet Med, Suez Canal University. . (1986): Aeromonas intestinal infections in the United States. Annals Of Internal Medicine ,105(5): 683-689. Janda, M.; Bottons, E.J and Reitano, M. (1983): Aeromonas species in clinical microbiology. Significance, epidemiology and speciation. Diagnostic Microbiology Of Infection Diseases, 1: 221-223. Krieg, N.R and Holt, J.G. (1984): Bergy's Manual of Systematic Bacteriology, the Williams and Wilkins, Baltmore, London. Manuel, P; Jose, M.R; Jesus, A.S and Maria-Lusia, G.L. (2009): Occurrence of motile Aeromonas in municipal drinking water and distribution of genes encoding virulence factors. International Journal of Food Microbiology, 135(2): 158-164. Maria Tereza , P. R; Marisa, D. B; Petra, S. S and Maria Ineˆ s, Z.S. (2008): Aeromonas detection and their toxins from drinking water from reservoirs and drinking fountains.
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