The phytochemical study of B. bituminosa revealed the presence of isoflavone (daidzin) and flavone (isoorientin) and identified for the first time in this specie. The antibacterial activity of the plant B. bituminosa is certainly related to its chemical content. The n-BuOH extract showed a significant antioxidant activity.
In this study, we focused on the isolation and identification of the main compounds, triterpene, sterol, phenolic and fatty acid from the aerial parts of Physospermum verticillatum, which has not been previously reported. Dichloromethane, ethyl acetate and n-butanol extracts led to the isolation of ß-amyrin (1), 3,11-dihydroxy-12-oleanene (2), pentadecanoic acid (3), spina-sterol (4), spinasterol-3-O-ß-D-glucopyranoside (5) and saikochromic acid (6). These compounds were determined by means of the combined systems with high resolution tandem mass spectrometry (HPLC-TOF/MS), 1D NMR ( 1 H NMR, 13 C NMR) and 2D NMR (COSY, HSQC and HMBC). Further, the extracts demonstrated significant antibacterial activity.
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