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Biopreservation of food using bacteriocin from lactic acid bacteria (LAB) was an innovative breakthrough. Lactic acid bacteria can protect against food spoilage and pathogen bacteria by producing bacteriocin. The purpose of this study was to characterize the bacteriocin produced by LAB isolated from solid waste of soymilk that had probiotics properties. The LAB having antibacterial activities was evaluated their growth, and identified by using 16S rRNA gene sequence analysis. Its bacteriocin activities was tested on various pHs (2, 3, 4, 5, 6, 7, 8, and 9) and temperature (60-100 ° C). Its activities was evaluated againts pathogenic bacteria (Staphylococcus aureus ATCC 25923 and Listeria. monocytogenes CFSAN004330), enzymes (trypsin, catalase and protease-K), and antibiotics (penicillin and ampicillin). The results showed that LAB A23.4 isolates, which had 16S rRNA gene sequence were L. plantarum strain TMW 1.1623. Its Bacteriocin had antimicrobial activity against S. aureus ATCC 25923 and L. monocytogenes CFSAN004330 at pH 2-7, at temperatures of 60, 70, 80, 90, 100 ° C for 60 minutes and lysed by the enzymes trypsin and protease-K. Bacteriosin activity was stronger than that of the antibiotics of penicillin and ampicillin against S. aureus and L. monocytogenes. The inhibition zone of supernatant bacteriocin was 10 and 20 mm for S. aureus and L. monocytogenes. On the other hand, penicillin and ampicillin inhibition zones were 0 and 3 mm, respectively. From these results, it can be concluded that the antimicrobial produced by L. plantarum strain TMW 1.1623 was a bacteriocin used as food preservation that its processing using relatively wide range temperature (60-100) with pH 2-7.

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Abstract. Aritonang SN, Roza E, Sandra A. 2020. Short Communication: Application of bacteriocin from Lactobacillus plantarum SRCM 1 004 34 strain isolated from okara as a natural preservative in beef sausage. Biodiversitas 21: 2240-2243. Lactic Acid Bacteria (LAB) can be found in food and most of the LAB have beneficial effects for humans as potential probiotics. LAB isolated from okara (soy milk pulp) produces antimicrobial compounds called bacteriocins which can be used as natural preservatives in sausages. This study aims to determine the effectiveness of this bacteriocin from LAB as a natural preservative in sausages. The bacteriocin was isolated from Lactobacillus plantarum SRCM 1 004 34 strain isolated from Okara. Completely Randomized Design (CRD) was used in 4 X 4 factorial pattern by three replicates The first factor was the percentage addition of bacteriocin (B): 0% (B0), 0.3% (B1), 0.6% (B2) and 0.9% v/b (B3). The second factor was the length of sausage storage time at 4°C (L): 0 days (L0), 4 days (L1), 8 days (L2) and 12 days (L3). The observed variables were moisture content, protein, fat, pH, total aerobic bacteria, and sausage fatty acid profile. The results showed that the content of protein, fat, and the bacterial count was significantly influenced by the interaction of the percentage addition of bacteriocin and storage time. pH was significantly affected by bacteriocin dose and storage time but was not affected by the interaction of other factors. The moisture content of the sausage was significantly affected by storage time. The results of this study showed that sausages treated with 0.9% bacteriocin (B3) with a maximum storage time of 12 in the refrigerator was still safe to be consumed.

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