A study on givotia rottlerifomis griff shows that studies on a tremendous of pharmacological and physiological activities and other applications. Moreover, givotia rottlerifomis griff has diverse physiological effects. This tempted us to use the bark of givotia rottlerifomis griff for extraction of new compound from the extract. Attempt is made to develop an efficient method for isolation of compounds from the barks of givotia rottlerifomis griff. Our scheme of work is to separate a portion by treatment with pure methanol (CH3OH) using soxhlet apparatus at boiling temperature and the sample is crystallized to separate to the pure compound. The compound was identified further by chromatographic studies, melting point determination, and spectral analysis (IR, 1D and 2D NMR) etc. Furthermore, antimicrobial studies are tested various antibacterial and antifungal species and also molecular docking studies are checked with HIV protease with Schrodinger 9.5 software.
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