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In the present study, the aerial parts of the Laperrine olive (Olea europaea subsp. Laperrinei) are subjected to acid extraction and the chemical composition of the extracts is determined by HPLC-DAD. The main compounds found in all of extracts are: hydroxytyrosol (30.45%), tyrosol (0.69%), oleuropein (32.76%), ferrulic acid (17.77%), quercetin (31.57%) and hesperetin (6.90%).The extracts obtained from the leafy stems of Laperrine olive tree are tested on the moth Ephestia kuehniella flour. Their administration by inhalation of newly exuviated chrysalises extends the duration of nymphalid development and disturbs the exuviated adults reproduction, by reducing the period in which the eggs are being laid. Thus, compared to the control insects, the number of eggs laid by treated females is significantly reduced after the treatment by extracts. Besides, the administration of different extracts of adult butterflies has a premature mortality effect.

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Background: Marrubium deserti de Noé, which is locally known as "Merriouet saharaui", is widely used in Algeria as a traditional treatment of many ailments. In this study, the anti-inflammatory and acute toxicity of the aqueous infusion extract from aerial parts of Marrubium deserti were investigated. Meanwhile, acute oral toxicity of M. deserti, as well as its anti-inflammatory activity is reported for the first time. Materials and Method:The anti-inflammatory activity was evaluated using carrageenin-induced paw oedema in mice at three different doses (250, 500 and 1000mg/kg body weight. Data were analyzed by one-way ANOVA followed by Dunnett's t-test. Results: The aqueous infusion extract (250, 500 and 1000mg/kg body weight, orally administered, n=6) showed a significant (P<0.05) inhibition of carrageenin-induced mice paw oedema by 11.22, 20.73 and 44.03% respectively in the third hour when compared to the control group. Acetylsalicylic acid (ASA, 50mg/kg) as the positive control showed 32.08% inhibition. The oral LD50 values in mice were found to be greater than 2000mg/kg. The relatively high oral median lethal dose (>2000mg/kg) suggests that the aqueous infusion extract has a relatively low acute toxicity when taken orally for a single dose. Conclusions:The present study indicates that M. deserti has a significant anti-inflammatory effect and confirms its traditional use as a treatment of pain, yet it suggests further investigations to be carried out to determine the active chemical constituents.

Salvia chudaei Batt. & Trab. from Algeria is traditionally used to relieve several dysfunctions, including inflammatory and pain-related situations.Aim of the study: This work aimed to confirm scientifically the referred properties. For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts. Materials and methods: Infusion of aerial parts of S. chudaei was prepared and screened for phenolic composition by generalized methods TPC and TFC then by LC-DAD-ESI/MSn. DPPH and FRAP were used to evaluate antioxidant activity. Using mice, acute toxicity, anti-inflammatory by carrageenan-induced paw edema, and analgesic by acetic acid-induced writhing and formalin-induced pain activities were tested. Results: The infusion showed 2018 mg GAE/100g DW of phenolics and 1956 mg ECE/100g DW of flavonoids. Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid. This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract. Additionally, the infusion exhibited a good antioxidant activity (DPPH: 81 μmol TE/g DW, FRAP: 438 μmol FSE/g DW). By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control. On the other hand, infusion up to 8 g/kg b.w. showed no signs of toxicity or mortality. Conclusion:This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, antiinflammatory, and analgesic activities, supporting the use of this species in folk medicine.