Anti-inflammatory and acute toxicity evaluation of aqueous infusion extract obtained from aerial parts of <i>Marrubium deserti</i> de Noé growing in Algeria

Abstract

Background: Marrubium deserti de Noé, which is locally known as "Merriouet saharaui", is widely used in Algeria as a traditional treatment of many ailments. In this study, the anti-inflammatory and acute toxicity of the aqueous infusion extract from aerial parts of Marrubium deserti were investigated. Meanwhile, acute oral toxicity of M. deserti, as well as its anti-inflammatory activity is reported for the first time. Materials and Method:The anti-inflammatory activity was evaluated using carrageenin-induced paw oedema in mice at three different doses (250, 500 and 1000mg/kg body weight. Data were analyzed by one-way ANOVA followed by Dunnett's t-test. Results: The aqueous infusion extract (250, 500 and 1000mg/kg body weight, orally administered, n=6) showed a significant (P<0.05) inhibition of carrageenin-induced mice paw oedema by 11.22, 20.73 and 44.03% respectively in the third hour when compared to the control group. Acetylsalicylic acid (ASA, 50mg/kg) as the positive control showed 32.08% inhibition. The oral LD50 values in mice were found to be greater than 2000mg/kg. The relatively high oral median lethal dose (>2000mg/kg) suggests that the aqueous infusion extract has a relatively low acute toxicity when taken orally for a single dose. Conclusions:The present study indicates that M. deserti has a significant anti-inflammatory effect and confirms its traditional use as a treatment of pain, yet it suggests further investigations to be carried out to determine the active chemical constituents.

Infusion of aerial parts of Salvia chudaei Batt. & Trab. from Algeria: Chemical, toxicological and bioactivities characterization

“…Lamiaceae (Labiatae) family that comprises 236 genera and about 6900-7200 species (Stevens, 2016;Tamokou et al, 2017) contains many species with medicinal virtues (Ruiz-Vargas et al, 2019;Saad et al, 2016;Sreeja et al, 2018). Salvia L. is the largest genus of this family (900-1000 species) (Walker et al, 2004); several Salvia species have very interesting pharmacological properties, namely antioxidant (Esmaeili and Sonboli, 2010), anti-diabetic (Flores-Bocanegra et al, 2017), analgesic (Simon-Arceo et al, 2017), in vitro/in vivo anti-inflammatory activity (Akram et al, 2015), and anti-Alzheimer effects (Senol et al, 2017), which in several cases are attributed to secondary metabolites, especially phenolic compounds.…”

Phytochemical Profiling and Evaluation of Marrubium sivasense Aytaç, Akgül & Ekici for Antioxidant Activity and Inhibition Effects on α-Amylase, Lipoxygenase, Xanthine Oxidase and Tyrosinase Enzymes

“…In the literature, there are a number of papers promising about phytochemical properties and biological activities of diverse Marrubium species (7,(11)(12)(13)(14)(15)(16)(17)(18)(19). However, there is no information about phytochemistry and biological potent of endemic species M. sivasense.…”

“…A previous phytochemical studies on Marrubium species resulted with polyphenols (7), essential oils (8), labdane diterpenoids and sterols (9,10). Biological activity investigations of Marrubium species encompasses antibacterial (11), antioxidant, antiproliferative (12), antinociceptive (13), antidiabetic (14), antihypertensive (15), hepatoprotective (16), antiinflammatory (17), cardioprotective (18) and cytotoxic/cytostatic (19) potentials. Some Marrubium species are traditionally used to treat various diseases, including asthma, pulmonary infections, inflammation and hypotension, as cholagogues and sedative agents, and for pain relief.…”

Evaluation of Acute Toxicity and Antiinflammatory Activity of Callus Extracts of Pulicaria Incisa (Lam.) Dc.

“…In the current study, the weighting of animals recorded an increase of body weight of mice in all control and assays tested with time; it was expressed by the moderate gaining of weight. Conversely, Jahn and Günzel (1997), also Saad et al (2016) described the toxicity sign by the weight loss. We consider that, saponins, mucilages, anthocyanins and flavonoids of callus are without any toxic prints on mice (Avachat et al, 2011;Du et al, 2015;Muthukumaran et al, 2017).…”