SUMMARYThe possibility of obtaining instant pure-breeding lines by matromorphic seed development in If. rustica has been investigated. Maternal parents homozygous for recessive marker genes and heterozygous for dominant marker genes were pollinated with different .Jficotiana species and with irradiated pollen of a X rust lea variety having a dominant marker. Poilinations with 65 species and with irradiated varietal pollen receiving doses ranging from 8 to 40 kr X-and gamma rays were performed on as many as 40 maternal genotypes.Promising matromorphic progenies were studied for the segregation of marker genes and several quantitative characters and for cytological abnormalities in the first and second generations following their induction, Of the numerous pollinations only one homozygous diploid maternal was recovered from F1 (V5 x Vi) maternal parent pollinated with N. tangsdorffi (2n = 18). Poilinations with 3v. langsdorffi, however, consistently and frequently induced haploids or near haploids. The evidence gathered over many years of experimentations throws serious doubt on the possibility of using matromorphy as an alternative method of producing pure-breeding lines in N. rustica.Transfer of a dominant marker gene from the irradiated pollen to a triple recessive maternal parent has prospective applications in the production of isogenie lines as an alternative to recurrent backcrossing.

The genetic determination of a quantitative component of pathogenicity, aggressiveness, was examined in progeny populations derived from three parent dikaryons of Ustilago hordei. Aggressiveness was assessed as the proportion of smutted plants produced from inoculated seed of a compatible barley cultivar. The parents differed in their origins and cultural histories and this was reflected in the variability of their progenies. A standard race-10 strain appeared to be homozygous for genes affecting aggressiveness, while a dikaryon produced by mating two unrelated sporidia was heterozygous and produced highly variable progeny populations. In these populations, aggressiveness was determined by a polygenic system which involved both additive and dominance gene effects. It was not possible to estimate the number of genes involved, but segregation was apparent in individual tetrads. Furthermore, a difference between opposite mating-type segregants suggested the presence of a factor which affected aggressiveness and was linked to mating type. The dominance effects were ambidirectional suggesting that genotypes giving an intermediate level of aggressiveness are the most fit. The aggressiveness of a pathogen strain is an important factor determining the severity of epidemics on compatible hosts. It is also a major component of fitness and may influence the frequency of virulence factors in pathogen populations and the evolution of new races.

The PCR-based technique of randomly amplified polymorphic DNA (RAPD) was used to fingerprint and assess the genetic relatedness among nine species of the fungus Alternaria isolated from various crop plants showing the leaf spot disease in Mosul, Iraq. Genomic DNA of each species was extracted at a final concentration of 300-400 µg / 2-3 g of wet mycelium , and at a purity of 1.6-1.8. Each DNA sample was amplified with each of 22 primers and the products were resolved electrophoretically on 1.2% agarose gel, stained with ethidium bromide and photographed under UV. One Primer failed to support amplification but the remaining 21 (95.5%) primers produced a total of 112 bands (2-10 per primer) across the nine species. Of these bands, 100 (1-10 per primer) were polymorphic. The least efficient primer was OP-H01 (1.79%), while the most efficient one weas OP-M05 (8.93 %). Primers OP-C05, OP-E20 and OP-T20 had the lowest (0.1%) discriminatory power while primer OP-M05 had the highest (10 %) power and identified all 9 species through unique patterns of banding. RAPD analysis fingerprinted eight of the nine isolates through marker bands with one or more of the 21 primers. Cluster analysis based on the genetic distances split the nine species into three distinct clusters with no obvious association between the pattern of clustering of the species and their host specificities.

This study was planned to investigate the probable protective role of Salvia officinalis have in the nanoparticles and aqueous extracts on female rats which exposed to Bisphenol A. At the end of the experiment the blood samples were collected by heart puncture to evaluate the RBC and WBC count, Hemoglobin, Platelets as well as follicle stimulating hormone (FSH), luteinizing hormones (LH), estrogen, creatinin, urea, aspartate aminotransferase, alanine amino transferees and alkaline phosphatase were estimated furthermore after the rats were scarified Liver, Kidney, Uterus and ovary were collected for histological study. The result shows significant decrease in the RBC, PLT and Hb count in the BPA group in comparison with the control and SOSNPs and SOE groups. In addition, there is a significant increase in WBC count and estrogen level in the BPA treated rats in comparison with control, SOSNP and SOE treated rats. As well as, there is significant increase in the liver enzymes (AST, ALP and ALP) in the BPA treated rats in comparison with control and SOSNP & BPA treated groups. Histological studies show presence of vacuolation with absence of radial arrangement in the hepatocytes in the liver of rats. The histological studies for the uterus of rats Beside ovarian sections BPA reveled mature follicle without oocyte with cyst like structure in the ovary in comparison with control group, these effects were relieved by the role of SOSNP and SOE in alleviating the effects of BPA on studied parameters.

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