Diseases resistant genes assemblage for tiger shrimp has been initiated by The Research Institute for Coastal Aquaculture in collaboration with Bogor Agricultural Institute, through transgenesis approach under anti-virus genes transfection. The study aimed to evaluate the growth performance of broodstock candidates of tiger shrimp at different generati on (F0 and F1). This research was conducted at 2000 m2size of four ponds in Takalar Regency, South Sulawesi.. The treatment was different generations of broodstocks, which were: broodstocks originated from F0generation, (A) and F1 (B). The 22.63 to 28.57 g of broodstock candidates were stocked 0.5 ind.m-2 and then reared for 128 days. During rearing period, these shrimp were fed usingcommercial pelleted feed with content 36-38% of protein in dosage of 10-4%/ body weight. Feeding frequency was applied in the morning and in the evening. Measured variables were growth, size distribution, survival rate and water quality The results indicated that the performances of these shrimps, growth, size distributions as well as survival rates between these F0 and F1 were not significantly different (p>0.05). The growth pattern was relatively equal between treatment during rearing period. Survival rate of tiger shrimp in this study ranged from 51.7 to 73.35%. This study have implications on the provision of superior broodstock shrimp in ponds in order to support the sustainability of shrimp seed production in hatchery. Keywords: broodstock, production, transgenic, tiger shrimp
The agricultural system in Indonesia is now more advanced by combining agriculture and fisheries on the same land, so research like this is carried out. This study aims to investigate the lethal concentration (LC50-48 hours) of the active ingredient biopesticide Azadirectha on tiger shrimp at the post-larvae stage, and to analyze the effect of Azadirectha biopesticide on the behavior of tiger prawn post larvae. The concentration of biopesticides used in the main test was determined from the results of 3 preliminary tests with an upper threshold value of 90,000 ppm and a lower threshold value of 30,000 ppm. Five biopesticide concentrations were used for the main test, i.e. 37,372 ppm, 46,555 ppm, 57,994 ppm, 72,244 ppm, 89,994 ppm, and control. The data analysis technique for LC 50 uses probit analysis. The results obtained by the lethal concentration (LC50-48 hours) with Azadirectha biopesticide on mortality of tiger shrimp post-larvae were 54,719.57 ppm, the effect of biopesticides on post larvae behavior of tiger prawns at a concentration of 40,000 ppm only stayed at the bottom and even died while with a maximum biopesticide. At the concentration of 37,372 ppm, the shrimp showed active behavior, making it a safe concentration limit for the shrimp to sustain a living.
Ocean acidification is one of an environment problem due to increasing CO2 concentration. Ocean acidification have shown a negatively impact to marine organisms especially calcifying organisms such as microalgae, coral reef and other invertebrate organisms. Decreasing in carbonate saturation state on the calcification rates of individual species and communities in both planktonic and benthic habitats were occurred due to seawater acidification.The study was conducted at three locations: Barru, Takalar and Barrang Lompo Island using mesocosm technique with two different of incubation periods (48 and 96 hours). Six pCO 2 level based on adding acid base treatments were used, which were 280, 380, 550, 650, 750 and 1000 ppm with 4 replicates In general, alkalinity decreased with increasing CO 2 concentration. However, length of incubation was showed no significant affected to DIC for all CO 2 concentration at Barru and Barrang Lompo mesocosm experiment. PIC and POC showed a varied response between all locations of mesocosms experiment. There was a trend where increasing of POC with increasing of CO 2 concentration. PIC concentration at Barru and Takalar showed a slightl higher at 96 hours than 48 hours of incubation period and for Barrang Lompo mesocosm experiment PIC was higher at 48 hours than 96 hours of incubation period.Chlorophyll a and cell abundance was decresing with increasing CO 2 concentration. Growth, photosynthesis and calcification rate decreased with increasing CO 2 concentration.
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