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In this study, three samples of olive oil wastes: Olive Rinse Water (OR), Olive Mill Wastewater (OM) and Olive Pomace (OP), which were collected from an olive oil mill located at Fez-Morocco, were analyzed for their microbiota. A total of 81 isolates were obtained and constituted a microbial bank formed of 35 bacteria, 41 yeasts and 5 fungi. The microbial communities have been compared in term of metabolic potential by testing hydrolytic enzymes activities of lipase, protease, amylase, cellulase, pectinase and tannase on agar plate media. The results reveal that among the examined microorganisms, 68 isolates were able to produce at least one of the screened enzymes. The pectinase activity was the predominant one (39.51%), followed by cellulase activity (34.57%) and by lipase activity (27.16%). However, the amylase activity was observed only for 11.11% of the studied isolates, followed by protease activity (9.88%) and by tannase activity 6.17%. Thereby, considering the isolates ability to produce enzymes, they can be considered as potential candidates for industrial and biotechnological applications. Lipases

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Recent improvements in 3D printing technology have increased the usage of 3D printed materials in several area. An exciting and emerging area of applying these next-generation manufacturing strategies is in the development of devices for biomedical applications. The main aim of this work was to investigate the effect of tannic acid, gallic acid and epicatechin gallate on the physicochemical characteristics of Acrylonitrile Butadiene-Styrene (ABS) and Nylon 3D printing materials. The results of the contact angle measurements showed a significant change in the physicochemical properties of both surfaces, indicated an increase in electron donor character of 3D printing materials following treatment. Thus, the ABS surfaces treated with tannic acid, gallic acid and epicatechin gallate have become more electron donating. Furthermore, the adhesion of Staphylococcus aureus on untreated and treated materials was evaluated by the scanning electron microscopy (SEM) analysis and the images were treated by MATLAB software. Our results proved the ability of S. aureus to adhere on all materials with a percentage of 77.86% for ABS and 91.62% for Nylon. The SEM has shown that all actives molecules were sufficient to obtain better inhibition of bacterial adhesion, which tannic acid has showed a total inhibition of S. aureus on ABS.

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