Objective: This study was to assess the effectiveness of educational program on nurses' knowledge level and their practices about nasogastric tube feeding at neonatal intensive care units. Methods: Settings: The study was conducted at Banha Specialized Pediatric Hospital and Banha University Hospital, from neonatal intensive care units. Research design: A quasi-experimental design. Sample: A convenient sample method of eighty three (83) as a one study group. Tools: The questionnaire format, the observation checklist and construction of educational program which were developed by the researchers to assessment for the knowledge and practice for nurses' related to nasogastric tube feeding. The educational program was designed serves as a referral instructional for nurses. Results: Implied that the educational program was having a positive effect, on enhance nurses' knowledge and practice related to nasogastric tube feeding at neonatal intensive care units. Conclusions: The present study recommended that an administrating a written protocol of nasogastric tube feeding to assure sufficient knowledge, and saved practices are really needed at neonatal intensive care units.