Aims: The aim of this study is to evaluate the effect of CPP−ACP, raw, fresh buffalo milk and cow milk on surface roughness of artificial initial caries lesions. Materials and Methods: 100 sound maxillary first premolar extracted for the purpose of orthodontic treatment had been collected and randomly divided into four groups, in all groups the teeth subjected to pH cycle procedure then treated with: Group1: n(25) deionized water, group2: n(25) CPP−ACP tooth mousse, group3: n(25) raw cow milk, group4: n(25) raw buffalo milk. Enamel surface was assessed by a profilometer device at a baseline and after demineralization and after remineralization. Results: In all groups, there was a high statistically significant increase in surface roughness after demineralization. And there was a high statistically significant decrease in surface roughness in all groups except deionized water after remineralization, the highest remineralization effect was found in CPP−ACP tooth mousse group followed by buffalo milk and then cow milk.Conclusions: Within the limits of the current study, it was concluded that CPP−ACP tooth mousse, raw fresh buffalo milk and cow milk were effective remineralizing agents which was reflected by decreasing surface roughness of artificial initial caries lesion, but with different potentials, CPP−ACP tooth mousse was the best followed by fresh raw buffalo milk which showed superior results in reducing surface roughness in comparison with fresh raw cow milk.
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