The phloem‐sucking psyllid Cacopsylla picta plays an important role in transmitting the bacterium ‘Candidatus Phytoplasma mali’, the agent associated with apple proliferation disease. The psyllid can ingest ‘Ca. Phytoplasma mali’ from infected apple trees and spread the bacterium by subsequently feeding on uninfected trees. Until now, this has been the most important method of ‘Ca. Phytoplasma mali’ transmission. The aim of this study was to investigate whether infected C. picta are able to transmit ‘Ca. Phytoplasma mali’ directly to their progeny. This method of transmission would allow the bacteria to bypass a time‐consuming reproductive cycle in the host plant. Furthermore, this would cause a high number of infected F1 individuals in the vector population. To address this question, eggs, nymphs and adults derived from infected overwintering adults of C. picta were reared on non‐infected apple saplings and subsequently tested for the presence of ‘Ca. Phytoplasma mali’. In this study it was shown for the first time that infected C. picta individuals transmit ‘Ca. Phytoplasma mali’ to their eggs, nymphs and F1 adults, thus providing the basis for a more detailed understanding of ‘Ca. Phytoplasma mali’ transmission by C. picta.
The psyllid species Cacopsylla melanoneura (Förster) and Cacopsylla picta (Förster) are vectors of 'Candidatus Phytoplasma mali', the causal agent of apple proliferation, one of the economically most important apple diseases in Europe. Both vectors are present in apple orchards of South Tyrol and Trentino provinces in Northern Italy. As no direct treatment of the disease is possible, monitoring of the psyllids provides information about the vector presence in the orchards and enables targeted control. Thus, fast and reliable identification of the various psyllids occurring in the apple orchards is required. Morphological differentiation is problematic due to extensive resemblance of some psyllid species especially among females and is error-prone for nymphs. Here we present a rapid and cost-effective polymerase chain reaction-restriction fragment length polymorphism method based on the cytochrome c oxidase subunit I region for the molecular identification of the vector species as well as eight further Cacopsylla species present in the orchards. This method was verified through 98.9% consensus with morphologically identified males, through sequencing and subsequent phylogenetic analysis. In case of doubtful morphological identification of females, the method was able to provide a refined species assignment and could also remarkably facilitate the identification of nymphs.
Alternaria sp. infections on apple (Malus × domestica Borkh.) lead to impaired fruit
quality and yield losses by leaf blotches and fruit spots, caused
by host-specific toxins (HSTs) of the Alternaria apple
pathotype like AM-toxins. Fungal isolates were obtained during severe
outbreaks on cv. Gala, Golden Delicious, and Cripps Pink(cov)/Rosy Glow(cov) in South Tyrol and other regions in northern
Italy. The isolates were tested for pathogenicity using in
vitro assays with detached apple leaves. Conidial suspensions
of pathogenic isolates were shown to provoke necrotic lesions also
in apple seedlings and on fruits. Detached-leaf assay-guided fractionation
of the isolates’ culture supernatant and a high-resolution
liquid chromatography-mass spectrometry (LC-MS) analysis tentatively
identified 27 known Alternaria phytotoxins and a
new putative toxin, (3ξ,4ξ,5ξ,6ξ,7ξ,11ξ)-3,6-dihydroxy-8-oxo-9-eremophilene-12-oic
acid (1). The constitution and the relative configuration
of the ring stereocenters of 1 were elucidated by NMR
spectroscopy, revealing unique structural features among Alternaria phytotoxins. Indeed, molecular analysis revealed the lack of the
toxin-related genes AMT1, AMT4,
and AMT14 in all isolates from the region, suggesting
that Alternaria apple blotch in the area was associated with another
metabolite (1).
Brown rot decay of stone and pome fruit caused by Monilinia spp. is an economically important disease. The main pathogens in Italy are the indigenous species Monilinia laxa and M. fructigena, and the invasive species M. fructicola. The presence of M. fructicola in Italy was reported for the first time in 2008 in Cuneo, Piedmont. Further records showed that M. fructicola spread and established rapidly in other regions of Italy. In South Tyrol, Europe's largest contiguous apple-growing area, the cultivation of stone fruit is an increasingly important agricultural branch for small-holder farmers of the mountainous areas. Up to now, it was unknown if M. fructicola is present and how M. laxa and M. fructigena are distributed. Therefore, plant protection strategies were applied without knowledge of the species present in the orchards. To detect the three Monilinia spp. on stone fruit trees and to determine their distribution, samples of fruit mummies were taken from scattered stone fruit trees and from sweet cherry orchards. A multiplex real-time PCR was applied to detect Monilinia spp. in the fruit mummies. The results showed the presence of M. fructicola for the first time in South Tyrol on three locations and that M. laxa was the predominant species, followed by M. fructigena.
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