Copper (Cu)-based fungicides have been used in viticulture to prevent downy mildew since the end of the 19th century, and are still used today to reduce fungal diseases. Consequently, Cu has built up in many vineyard soils, and it is still unclear how this affects soil functioning. The present study aimed to assess the short and medium-term effects of Cu contamination on the soil fungal community. Two contrasting agricultural soils, an acidic sandy loam and an alkaline silt loam, were used for an eco-toxicological greenhouse pot experiment. The soils were spiked with a Cu-based fungicide in seven concentrations (0–5000 mg Cu kg−1 soil) and alfalfa was grown in the pots for 3 months. Sampling was conducted at the beginning and at the end of the study period to test Cu toxicity effects on total microbial biomass, basal respiration and enzyme activities. Fungal abundance was analysed by ergosterol at both samplings, and for the second sampling, fungal community structure was evaluated via ITS amplicon sequences. Soil microbial biomass C as well as microbial respiration rate decreased with increasing Cu concentrations, with EC50 ranging from 76 to 187 mg EDTA-extractable Cu kg−1 soil. Oxidative enzymes showed a trend of increasing activity at the first sampling, but a decline in peroxidase activity was observed for the second sampling. We found remarkable Cu-induced changes in fungal community abundance (EC50 ranging from 9.2 to 94 mg EDTA-extractable Cu kg−1 soil) and composition, but not in diversity. A large number of diverse fungi were able to thrive under elevated Cu concentrations, though within the order of Hypocreales several species declined. A remarkable Cu-induced change in the community composition was found, which depended on the soil properties and, hence, on Cu availability.
A b s t r a c t. Agricultural intensification, especially enhanced mechanisation of soil management, can lead to the deterioration of soil structure and to compaction. A possible amelioration strategy is the application of (structural) lime. In this study, we tested the effect of two different liming materials, ie limestone (CaCO 3 ) and quicklime (CaO), on soil aggregate stability in a 3-month greenhouse pot experiment with three agricultural soils. The liming materials were applied in the form of pulverised additives at a rate of 2 000 kg ha -1 . Our results show a significant and instantaneous increase of stable aggregates after quicklime application whereas no effects were observed for limestone. Quicklime application seems to improve aggregate stability more efficiently in soils with high clay content and cation exchange capacity. In conclusion, quicklime application may be a feasible strategy for rapid improvement of aggregate stability of fine textured agricultural soils.
Nitrification inhibitors (NIs) have been shown to reduce emissions of the greenhouse gas nitrous oxide (n 2 O) from agricultural soils. However, their N 2 O reduction efficacy varies widely across different agroecosystems, and underlying mechanisms remain poorly understood. To investigate effects of the NI 3,4-dimethylpyrazole-phosphate (DMPP) on N-turnover from a pasture and a horticultural soil, we combined the quantification of N 2 and N 2 O emissions with 15 N tracing analysis and the quantification of the N 2 O-reductase gene (nosZ) in a soil microcosm study. Nitrogen fertilization suppressed nosZ abundance in both soils, showing that high nitrate availability and the preferential reduction of nitrate over N 2 O is responsible for large pulses of N 2 O after the fertilization of agricultural soils. DMPP attenuated this effect only in the horticultural soil, reducing nitrification while increasing nosZ abundance. DMPP reduced N 2 O emissions from the horticultural soil by >50% but did not affect overall n 2 + N 2 O losses, demonstrating the shift in the N 2 o:n 2 ratio towards N 2 as a key mechanism of N 2 O mitigation by NIs. Under non-limiting NO 3 − availability, the efficacy of NIs to mitigate N 2 O emissions therefore depends on their ability to reduce the suppression of the N 2 O reductase by high NO 3 − concentrations in the soil, enabling complete denitrification to N 2 .Agricultural soils have become the main source of anthropogenic nitrous oxide (N 2 O), a powerful greenhouse gas and the single most important substance depleting stratospheric ozone 1 . Delaying the conversion of ammonium (NH 4 + ) to nitrate (NO 3 − ), nitrification inhibitors (NIs) have been suggested as a means to reduce N 2 O emissions from agricultural soils. NIs demonstrated their efficacy across different cropping soils 2 , but results vary widely, and in particular in pasture soils the use of NIs had no or little effect on N 2 O emissions 3-5 . Despite a growing body of research on NIs, mechanisms and factors determining their efficacy to reduce N 2 O emission remain poorly understood 6 . The challenges to understand these mechanisms derive from the fact that N 2 O is formed via several different pathways in the soil matrix 7 , tightly coupled to different processes of N supply and consumption 8 . Critically, N 2 O can be further reduced to N 2 via the microbial-mediated process of denitrification, and the sole quantification of N 2 O as affected by NIs provides therefore only a limited insight into mechanisms of N 2 O mitigation using NIs.Microbial metabolic pathways can contribute via a wealth of different processes to N 2 O production and consumption, i.e. the reduction to N 2 in soils. Apart from abiotic processes, N 2 O formation can be categorized into www.nature.com/scientificreports www.nature.com/scientificreports/ nitrification-mediated pathways, denitrification and biotic formation of hybrid N 2 O 9 . Denitrification is generally assumed to be the main process contributing to overall N 2 O production from agricultu...
Using zeolite-rich tuffs for improving soil properties and crop N-use efficiency is becoming popular. However, the mechanistic understanding of their influence on soil N-processes is still poor. This paper aims to shed new light on how natural and NH4+-enriched chabazite zeolites alter short-term N-ammonification and nitrification rates with and without the use of nitrification inhibitor (DMPP). We employed the 15N pool dilution technique to determine short-term gross rates of ammonification and nitrification in a silty-clay soil amended with two typologies of chabazite-rich tuff: (1) at natural state and (2) enriched with NH4+-N from an animal slurry. Archaeal and bacterial amoA, nirS and nosZ genes, N2O-N and CO2-C emissions were also evaluated. The results showed modest short-term effects of chabazite at natural state only on nitrate production rates, which was slightly delayed compared to the unamended soil. On the other hand, the addition of NH4+-enriched chabazite stimulated NH4+-N production, N2O-N emissions, but reduced NO3−-N production and abundance of nirS-nosZ genes. DMPP efficiency in reducing nitrification rates was dependent on N addition but not affected by the two typologies of zeolites tested. The outcomes of this study indicated the good compatibility of both natural and NH4+-enriched chabazite zeolite with DMPP. In particular, the application of NH4+-enriched zeolites with DMPP is recommended to mitigate short-term N losses.
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