Aim This study aimed to analyse bacterial community and biomineralization products from Wudalianchi non‐active volcanic field and the relationship between magnetization and bacterial community. Methods and Results Eighteen sediment samples obtained from Wenbo Lake, high‐throughput sequencing and quantitative PCR (qPCR) were separately employed to investigate the bacterial community composition dynamics and abundance variation of the sediment sample with the highest iron‐reducing capacity during incubation. The mineralization products were characterized by transmission electron microscopy, scanning electron microscopy, X‐ray diffraction (XRD), Raman spectroscopy, vibrating sample magnetometer (VSM) and variable‐temperature magnetism analyses. The results showed that the highest iron reduction rate was 98·06%. Seven phyla were identified as dominant bacterial phyla during the incubation process. Iron‐reducing bacteria (FeRB) including Geobacter, Desulfosporosinus and Clostridium were involved in the iron mineralization process. The 16S rDNA copy numbers of sediment decreased quickly and then stayed steady during the incubation. Bacteria with rod‐shaped and spheroid species were involved in extracellular iron reduction to produce magnetic particles with massive aggregation and columnar structures on the mineral surface morphologies. The materials produced by the microbial community over the incubation period were sequentially identified as siderite, magnetite and maghemite. The magnetism of the mineral samples gradually increased from 0·31748 to 33·58423 emu g−1 with increased incubation time. The final products showed relatively stable magnetism under 0–400 K. Meanwhile, the saturation magnetization (MS) of the mineralized substance was tightly associated with bacterial diversity (P < 0·05). Conclusions Bacterial community varied during incubation of iron‐reducing sediment of volcanic lake. Various iron mineral crystals were in turn formed extracellularly by FeRB. The magnetism of mineralized products was tightly associated with bacterial community. Significance and Impact of the Study These results not only help us to better understand the iron mineralization of FeRB in the volcanic lake sediments but also provide basic information for the future application of FeRB in environmental bioremediation.
The effect of heat treatment on solubility, surface hydrophobicity and structure of rice bran albumin and globulin were characterised. The differential scanning calorimetry (DSC), FT-IR and Raman spectra were used to analyse the effect. The results of DSC analysis showed that albumin denatured after a thermal pre-treatment of 70 °C, while globulin denatured at 90 °C. The solubility of albumin sharply decreased after heat treatment, while the solubility of globulin decreased by up to 100 °C. The results of FT-IR demonstrated that heating globulin generally increased the content of α-helix and β-turn structure, but reduced β-sheet and random coil structures, while heating albumin produced a pronounced increase in α-helix and β-sheet structures from random coil and β-turn structures. Heating albumin decreased regions around 33 kDa but increased relative band intensity around 45-53 kDa in SDS-PAGE pattern, while heating globulin up to 90 °C caused losses in bands around 20 and 50 kDa. Raman spectrogram showed that heating albumin induced a slight decrease in intensity of the Trp band. However, the Trp band of heated globulin did not change.
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