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Abstract.A new virus named Sitiawan virus (SV) was isolated from sick broiler chicks in chicken embryos. The virus replicated well with cytopathogenic effect (CPE) in the chicken B-lymphocyte cell line LSCC-BK3. The virus was an enveloped RNA virus of approximately 41 nm in size with hemagglutinating activity (HA) to goose erythrocytes. It was cross-reactive with Japanese encephalitis virus (JEV), a member of flaviviruses by HA inhibition tests but not by cross-virus neutralization tests. The cDNA fragment of NS5 gene was amplified with primers corresponding to NS5 gene of flaviviruses. The nucleotide sequences were 92% homologous to Tembusu virus, a member of the mosquito-borne virus cluster of the genus Flavivirus. In cross-neutralization tests with Tembusu virus, antiserum to SV did not neutralize Tembusu virus, and antiserum to Tembusu virus neutralized more weakly to SV than against homologous virus. These results indicate that SV is a new virus which can be differentiated serologically from Tembusu virus but is otherwise similar with respect to nucleotide sequence. The virus causes encephalitis, growth retardation, and increased blood glucose levels in inoculated chicks.

The rectal content of an apparently normal 1-week-old broiler chick yielded an unclassified cytopathogenic virus with cytopathic effects of the round type. It was identified as a picornavirus from the following: ribonucleic acid in the viral core; virus growth in the cytoplasm; a particle about 30 nm in diameter; resistance to ethyl ether, chloroform, trypsin, and acid; relative heat-lability; and partial stabilization to molar magnesium chloride. The virus was stable under freezing and thawing, and sonication. It was distinguished from avian encephalomyelitis virus by the neutralization test.

In this study, fatal RP tended to be more common in the patients with subclinical ILD. In particular, the presence of extensive fibrosis on CT may be a contraindication for thoracic RT.

The pathogenicity of the G-4260 strain of picornavirus for day-old chicks was studied by intraperitoneal inoculation. No clinical signs were observed. A mild yellowish-tan discoloration of the kidneys was noticed in necropsy 7 to 21 days after inoculation. Mean body weight was significantly lower (P less than 0.01) in inoculated groups than in control groups 7 days after inoculation. In a chronological study on the distribution of the virus in organs, the virus was recovered from various organs, exclusive of the brain and trachea. The virus titer was higher in the kidneys, jejunum, rectum, and bursa of Fabricius than in any other organ. Fluorescent antigens were seen predominantly in the epithelia of the renal tubules.

The purpose of this study was to assess the efficacy and toxicity of definitive radiotherapy (RT) for the recurrence of epithelial ovarian cancer, which is limited to one or two gross regions, after complete remission had been achieved with aggressive front-line therapy. Twenty-seven patients were treated with definitive RT and were retrospectively analyzed. Their median tumor size was 3.0 cm. Twenty-six (96%) patients received external irradiation at a median total dose of 60 Gy, and a median daily dose of 2 Gy. Only two patients received intracavitary brachytherapy. Twenty (74%) of the 27 patients received systemic chemotherapy for the treatment of a limited recurrent tumor followed by definitive RT. Six (22%) of the patients received concurrent chemotherapy and seven (26%) of the patients also underwent regional hyperthermia during definitive RT. Twenty-two (82%) patients had an objective response (CR: 11, PR: 11). The 2-year overall survival, progression-free survival and local (in-field) control rates after RT were 53%, 39% and 96%, respectively. The toxicities were mild, no Grade 3 or higher toxicity was observed in any of the patients. The tumor size( < 3 cm), period between front-line therapy and RT (≥2 year) and objective tumor response (CR) were significant prognostic factors of the overall survival rate. In conclusion, definitive RT for limited recurrence of epithelial ovarian cancer achieves a better local control rate without severe toxicity, and it may therefore be a potentially effective modality for inducing long-term survival in selected patients.

These data suggest that mast cells play an important role in the onset of airway hyperreactivity but do not play a role in the production of IL-5 and eosinophilia. Furthermore, indicate that the inhibition of IL-5 is not always associated with a reduction in antigen-induced airway hyperreactivity in mice.

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