Characterization of a Picornavirus Isolated from Broiler Chicks

Yamaguchi, S; Imada, Tadao; Kawamura, Hikaru

doi:10.2307/1589732

Cited by 85 publications

(43 citation statements)

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“…Avian Nephritis Virus (ANV) is an enteric virus, which primarily infects chickens, but has also been detected in turkeys and pigeons (Yamaguchi et al, 1979;Connor et al, 1987;Zhao et al, 2011). Its initial discovery was made in 1976 in Japan and it has subsequently been detected in other countries including in Australia in 2008 (Yamaguchi et al, 1979;Hewson et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Its initial discovery was made in 1976 in Japan and it has subsequently been detected in other countries including in Australia in 2008 (Yamaguchi et al, 1979;Hewson et al, 2010). Experimental infection studies have suggested that the virus can cause poor growth, kidney lesions and increased mortality in young chickens (Frazier et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

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High-resolution melt curve analysis to confirm the presence of co-circulating isolates of avian nephritis virus in commercial chicken flocks

et al. 2015

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Avian Nephritis Virus (ANV) has been implicated in poor growth and renal disease of young chickens. This paper describes the development of a reverse-transcriptase polymerase chain reaction for the detection of ANV in commercial meat chickens and the use of high-resolution melt curves to detect the presence of genetically different ANVs. Pooled cloacal swabs from both healthy and ill commercial chicken broiler flocks were tested for the presence of ANV using a combination of polymerase chain reaction, molecular cloning, high-resolution melt curve analysis and sequencing. Except for one, all specimens were found to contain two genetically different ANVs. Phylogenetic analysis of the capsid amino acid sequences revealed the presence of four of six groups of ANV identified previously in other countries as well as in two novel groups of ANV. Phylogenetic analysis of nucleotide sequences of partial polymerase, capsid and 3′ untranslated regions reveal that the genes of individual ANV virus isolates have different ancestors. This was shown to be due to a template-switching event in the capsid gene that resulted in the 3′ end of the capsid gene and the 3′ untranslated region of one ANV isolate being transferred to another ANV. These results reveal that infection of chicken flocks with multiple ANV isolates is common and this needs to be taken into consideration in diagnosis of ANV using molecular techniques and in future epidemiological investigations.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High-resolution melt curve analysis to confirm the presence of co-circulating isolates of avian nephritis virus in commercial chicken flocks

et al. 2015

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“…To date, two different astrovirus species have been identified in chickens. Avian nephritis virus (ANV-1) was originally isolated from a 1-week-old healthy broiler chick by Yamaguchi et al (1979) and has now been classified as an astrovirus after complete sequencing of the viral genome (Imada et al, 2000). This virus causes mild growth depression, interstitial nephritis and sometimes even mortality, especially after infection of 1-day-old specific pathogen free (SPF) chickens.…”

Section: Introductionmentioning

confidence: 99%

Detection and characterization of a new astrovirus in chicken and turkeys with enteric and locomotion disorders

Wit

Dam²,

Laar³

et al. 2011

Avian Pathology

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In the present paper, we report the unexpected discovery of a new virus in samples from chicken and turkey flocks with clinical disorders such as tenosynovitis, enteric problems, or runting and/or stunting-like conditions. Since 1987, several virus isolation attempts on samples from these flocks resulted in the same macroscopic characteristic lesions in embryonated specific pathogen free eggs, being mortality with brightred discolouration of legs and wing-tips, a swollen dark-red liver and oedema. Initial work suggested the presence of an agent with characteristics of a non-enveloped RNA virus. Further work, which is described in this paper, showed that the isolated strains formed a new group of avian nephritis viruses, which is genetically and antigenically distinct from known avian astroviruses. Inoculation of a representative strain (isolate 19) of this new group of avian nephritis viruses, provisionally named avian nephritis virus-3, in specific pathogen free layer chicks resulted in diarrhoea, runting and stunting, and even mortality.

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“…The isolation of enteroviruses and enterovirus-like viruses from chickens has been reported previously (Butterfield et al, 1969;Yamaguchi et al, 1979;McNulty et al" , 1987Spackman et al, 1984;Decaesstecker et al, 1986;Takase et al, 1989;Shirai et al, 1992). Several of these viruses have been compared by cross-indirect immunofluorescence and cross-serum neutralization tests and 5 serogroups identified (Takase et al, 1989;McNulty et al, 1990;Shirai étal, 1991Shirai étal, , 1992.…”

Section: Discussionmentioning

confidence: 99%

“…These include avian nephritis virus (ANV) which was first isolated from the rectal contents of a clinically normal broiler chicken in Japan (Yamaguchi et al, 1979), two enterovirus-like viruses (ELV-1, ELV-2) isolated from the faeces of broiler chickens , a virus (BELV-1) isolated from broiler chickens with runting stunting syndrome in Belgium (Decaesstecker et al, 1986) and FP3 virus, isolated from the meconium of dead-in-shell chicken embryos in England (Spackman et al } 1984).…”

Section: Introductionmentioning

confidence: 99%

Studies on a new enterovirus‐like virus isolated from chickens

et al. 1994

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SUMMARYDuring investigations into an outbreak of respiratory distress in broilers chicks, a small round virus was isolated following inoculation into chicken embryos. The isolate, designated 612, was identified as an enterovirus-like virus on the basis of its size and morphology, resistance to chloroform and to treatment at pH 3.0, and intracytoplasmic replication in cell culture. The virus produced a partial cytopathic effect following inoculation into chick embryo kidney cell cultures and viral antigens could be detected by immunostaining. The preferred culture method for 612 virus was by inoculation onto the CAM of chick embryos. Cross-immunofluorescence indicated that the virus is not antigenically related to five previously identified chicken enterovirus/enterovirus-like virus serogroups. Following experimental inoculation of 1-day-old male broilers a number of which had maternal antibody to 612, growth retardation ranging from 9.6 to 20.4% was detected. Serological studies demonstrated antibody to 612 virus was widespread in commercial chicken flocks in N. Ireland.

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Characterization of a Picornavirus Isolated from Broiler Chicks

Cited by 85 publications

References 1 publication

High-resolution melt curve analysis to confirm the presence of co-circulating isolates of avian nephritis virus in commercial chicken flocks

High-resolution melt curve analysis to confirm the presence of co-circulating isolates of avian nephritis virus in commercial chicken flocks

Detection and characterization of a new astrovirus in chicken and turkeys with enteric and locomotion disorders

Studies on a new enterovirus‐like virus isolated from chickens

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