A simple, precise, accurate, and rapid reverse phase-high performance liquid chromatography (RP-HPLC) method with UV-Visible detector has been developed and subsequently validated for the simultaneous determination of amlodipine besylate(AML) and celecoxib(CEL) in their combined tablet dosage form. The separation was based on the use of a Flowrosil C18 analytical column (250 × 4.6 mm, i.d., 5 µm). The mobile phase consisted of a mixture of 80 volumes of acetonitrile and 20 volumes of water. The chromatography was performed by isocratic elution at a flow rate of 1 mL/min. Analytes were detected at 250 nm, with linear calibration curves at concentration ranges of 2-12 µg/ml and 50-300 µg/ml for AML and CEL respectively. The retention time of AML and CEL were 1.98 and 3.15 min respectively. The recoveries obtained were 99.46‒101.36% for AML, 99.57‒101.42% and 99.96–100.87 % for CEL. The method was validated according to International conference of harmonisation guidelines in terms of accuracy, precision, specificity, robustness, limits of detection and quantitation, and other aspects of analytical validation. The developed method was applied successfully for HPLC analysis of commercial pharmaceutical products including AML and CEL.
Keywords: Amlodipine besylate; Celecoxib; RP-HPLC.
Copper based shape memory alloys are the alloys prepared with the combination of 66-88 wt% of copper, 10-14 wt% of aluminum, 0.3-0.6 wt% of beryllium and 0.1-0.4 wt% of manganese in the induction furnace through ingot metallurgy. The prepared SMAs is subjected to homogenization, it was observed that the samples exhibits Beta phase at high temperature and shape memory effect after going through step quenching to a low temperature. Microstructure and Shape memory effect was studied with the help of optical microscope and bend test respectively. It was seen that with increase in wt % of manganese the shape memory effect also increases.
The aim of the present study was an attempt to prepare tablets containing inclusion complexes of meloxicam with β-cyclodextrins and hydroxypropyl-β-cyclodextrin to improve the aqueous solubility of the drug, thus enhances its dissolution rate to show a faster onset of action. Complexation with cyclodextrins has been used as novel approach for designing drug delivery system because of ability for the non-covalent inclusion complexes formation and it has the ability to molecular encapsulate meloxicam into their hydrophobic cavity without the formation of any covalent bonds. Meloxicam complexation with cyclodextrin enhance the drug solubility and dissolution rate. Also cyclodextrins enhance the bioavailability of insoluble drugs by increasing the drug solubility, dissolution and permeability. The carriers used in this study are β-cyclodextrin and hydroxypropyl-β-cyclodextrin. Solid complexes of meloxicam with β-cyclodextrin and hydroxypropyl-β-cyclodextrin were prepared by two different methods namely, Kneading and Co-evaporation techniques. For kneading method, water-methanol-dichloromethane at 2 : 5 : 3 ratio was used as a solvent blend and for co-evaporation techniques, methanol-dichloromethane was used as solvent blend at 2 : 3 ratio. In each case different proportions of drug and carriers at 90 : 10, 75 : 25, 50 : 50, 25 : 75, 10 : 90 were used in the preparation of solid complexes to evaluate the effect of carrier concentrations on the dissolution characteristics. A total of 20 meloxicam solid complexes with different carriers were prepared, found to be fine and free flowing powders. The estimated drug content of the prepared complexes were in the range of 100 ± 5%. The dissolution parameters of T50 and dissolution rate indicated rapid dissolution of meloxicam from the solid complexes when compared with the pure drug and physical mixture. Meloxicam-β-CD (10 : 90) complex gave the highest dissolution rate.
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