Cultured human umbilical cord mesenchymal stem cells (hUC-MSCs) are being tested in several clinical trials and encouraging outcomes have been observed. To determine whether in vitro expansion influences the genomic stability of hUC-MSCs, we maintained nine hUC-MSC clones in long-term culture and comparatively analyzed them at early and late passages. All of the clones senesced in culture, exhibiting decreased telomerase activity and shortened telomeres. Two clones showed no DNA copy number variations (CNVs) at passage 30 (P30). Seven clones had ≥1 CNVs at P30 compared with P3, and one of these clones appeared trisomic chromosome 10 at the late passage. No tumor developed in immunodeficient mice injected with hUC-MSCs, regardless of whether the cells had CNVs at the late passage. mRNA-Seq analysis indicated that pathways of cell cycle control and DNA damage response were downregulated during in vitro culture in hUC-MSC clones that showed genomic instability, but the same pathways were upregulated in the clones with good genomic stability. These results demonstrated that hUC-MSCs can be cultured for many passages and attain a large number of cells, but most of the cultured hUC-MSCs develop genomic alterations. Although hUC-MSCs with genomic alterations do not undergo malignant transformation, periodic genomic monitoring and donor management focusing on genomic stability are recommended before these cells are used for clinical applications.
Recent observations of the light component of the cosmic-ray spectrum have revealed unexpected features that motivate further and more precise measurements up to the highest energies. The Dark Matter Particle Explorer (DAMPE) is a satellite-based cosmic-ray experiment that is operational since December 2015, continuously collecting data on high-energy cosmic particles with very good statistics, energy resolution, and particle identification capabilities. In this work, the latest measurements of the energy spectrum of proton+helium in the energy range from 46 GeV to 316 TeV are presented. Among the most distinctive features of the spectrum, a spectral hardening at ∼600 GeV has been observed, along with a softening at ∼29 TeV measured with a 6.6σ significance. Moreover, by measuring the energy spectrum up to 316 TeV, a strong link is established between space-and ground-based experiments, also suggesting the presence of a second hardening at ∼150 TeV. * https://geant4.web.cern.ch/node/302 † https://web.ikp.kit.edu/rulrich/crmc.html
OBJECTIVE: To investigate the effects of mandibular defects repaired by a tissue engineered bone complex with b-tricalcium phosphate (b-TCP) and bone morphogenic protein-2 (BMP-2) gene-modified bone marrow stromal cells (bMSCs). MATERIALS AND METHODS: bMSCs derived from Fisher 344 rats were cultured and transduced with adenovirus AdBMP-2, AdEGFP gene in vitro. Osteogenic differentiation of bMSCs was determined by alkaline phosphatase staining, von Kossa assay and reverse transcription-polymerase chain reaction. Gene transduced or untransduced bMSCs were seeded on b-TCP scaffolds to repair mandibular full thickness defects with a diameter of 5 mm. Eight weeks post-operation, X-ray examination, micro-computerized tomography and histological and histomorphological analysis were used to evaluate the bone healing effects. RESULTS: Alkaline phosphatase staining and mineralized nodules formation were more pronounced in AdBMP-2 group 14 days after gene transduction when compared with that of AdEGFP or untransduced group. The mRNA expression of osteopontin and osteocalcin also significantly increased 9 days after AdBMP-2 gene transduction. Mandibular defects were successfully repaired with AdBMP-2-transduced bMSCs ⁄ b-TCP constructs. The percentage of new bone formation in AdBMP-2 group was significantly higher than that of other control groups. CONCLUSIONS: Bone morphogenic protein-2 regional gene therapy together with b-TCP scaffold could be used to promote mandibular repairing and bone regeneration. Oral Diseases (2010) 16, 46-54
A study was conducted to determine the potential suitability of Terra/MODIS imagery for monitoring short-term phenological changes in forage conditions in a semi-arid region. The study sites included four meadow steppes and six typical steppes in the Xilingol steppe in central Inner Mongolia, China. The live biomass, dead standing biomass, total biomass, crude protein (CP) concentration and standing CP were estimated from early April to late October using the Enhanced Vegetation Index (EVI) values from Terra imagery (500 m pixels). Applying regression models, the EVI accounted for 80% of the variation in live biomass, 42% of the dead biomass, 77% of the total biomass, 11% of the CP concentration and 74% of the standing CP. MODIS/EVI is superior to AVHRR/NDVI when estimating forage quantity. Applying these results, the seasonal changes in live biomass and the standing CP could be described in the selected four sites with different degrees of grazing intensity. Generally, the increase in grazing intensity tended to decrease live biomass and standing CP. It was suggested that the EVI obtained from Terra imagery was an available predictor of the forage condition as measured by live biomass and standing CP. The MODIS/EVI values could provide information on the suitable timing of cutting for hay-making and nutritive value to range managers.
We report a histologically verified case of ameloblastic fibro-odontosarcoma (AFOS) affecting the right mandible. CT scanning showed an expansile intraosseous mass with ill-defined borders and slightly dense opacities.
Background: Although high-fructose corn syrup (HFCS) as a kind of sugar has been widely used in manufactured foods recently, there is little information available regarding its cariogenicity. The aim of this study was to evaluate the cariogenic potential of HFCS. Methods: Streptococcus mutans UA159 was inoculated into HFCS media and cultivated. The pH of each culture was measured to assess acidogenicity. Spectrophotometric turbidity was measured to determine the percentage of adherence. Confocal laser scanning microscopy and SYTO-9 staining were employed to observe biofilm formation. Sucrose media was used as a positive control. Results: The DpH in HFCS media was significantly larger than that in sucrose media and the pH in HFCS media decreased faster (p < 0.05). The percentage of adherence of S. mutans in HFCS media was significantly lower than that in sucrose media (p < 0.05). The biofilm formed in sucrose media was significantly thicker than that in HFCS media (p < 0.05). Conclusions:The results of this study suggest that the cariogenicity of S. mutans in the presence of HFCS may differ compared to its cariogenicity in the presence of sucrose. Further in vivo studies need to be undertaken to resolve this uncertainty.
Adhesion molecules are involved in cell invasion in autoimmune thyroid disease. It was also reported that patients with untreated Graves' disease (GD) had high serum level of soluble form of E-selectin (sE-selectin), the concentration of which correlated with the activity of the disease. The aim of the present study was to elucidate whether the common variants in Eselectin gene (SELE) were associated with the development of GD. Six tagSNPs within SELE were studied in 297 patients with GD and 208 healthy subjects in Chinese population. Our data showed that common SELE variants were associated with GD (P ¼ 0.012-0.036). Haplotype analysis of the single nucleotide polymorphisms revealed an association of a haplotype ATAACC with GD (P ¼ 0.005). Furthermore, quantitative trait analysis showed a significant association of SELE haplotype with sEselectin levels (P ¼ 0.0438). This study therefore could provide us to a certain degree the insight that common SELE variants may be associated with susceptibility to GD in Chinese population, though the limitation of sample size and multiple test problems exists.
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