Phosphate is an essential macro element that has a necessary function as a constituent of ATP and DNA in plants. However, the availability of dissolved phosphate in the soil is minimal because it tends to bind with soil minerals to form phosphate complexes. With rhizosphere fungi, the low available phosphate in the soil can be overcome. This study aimed to determine the character and potential of fungi capable of dissolving phosphate. The source of the isolates used was a collection of rhizosphere fungus isolates under the red jabon stand. Purification was carried out using the point method on PDA media. The phosphate dissolving ability test was done using the standard method using liquid pikovskaya media and then analyzed descriptively and quantitatively. Eighteen rhizosphere fungus isolates were observed, two of which could dissolve phosphate, respectively, obtained from isolates JCS16 with a concentration value of 10.48 ppm, JCS 13 with a concentration value of 10.06 ppm.
Open-pit mining activities cause land degradation; therefore, post-mining land recovery or reclamation is essential. An indicator to rate the success of mine reclamation activities is the diversity of soil microbial populations. Molecular bacteria identification requires prior information about the amplification of universal primers for molecular analysis. This study analyzes the amplification of 16S rRNA primers on rhizosphere bacteria isolates from reclamation and forest areas. The research activities encompassed sample preparation, isolation of bacteria isolates, isolation of DNA isolate, quantitative test, qualitative test, and amplification. The findings showed that pure isolates of rhizosphere bacteria from reclamation and natural forest areas that could be used were five and ten isolates, respectively. One isolate (20%) from the reclamation area and four isolates (40%) from natural forest generated DNA band, which were amplified using 16S rRNA.
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