Rhizosphere is a part of the soil that is in the roots of plants in which there are many soil microorganisms. One of the microorganisms found in the rhizosphere is fungi. Rhizosphere fungus plays an important role in increasing plant growth by various mechanisms that are carried out such as increasing nutrient absorption, as a bilogical control of pathogenic attacks and can produce growth hormones for plants. This study aims to identify and get the information of the diversity of rhizosphere fungi from mahogany stands in two provenances, and get information on IAA level production. The research methods include isolation of fungi and identification of rhizosphere fungi, as well as test of IAA production capability qualitatively and quantitatively. The results showed 17 rhizosphere fungus isolates were found under the mahogany stand in Takalar District those were included in the genus Rhizopus, Fusarium, Aspergillus, Penicillium and Gliocladium, whereas 11 Mahogany stands in the Maros Regency were included in the genus Trichoderma, Gliocladium, Rhizopus and Aspergillus. The whole genus is capable of producing IAA hormone, but the genus capable of producing the highest IAA is the Trichoderma genus.
The successful of genomic DNA amplification using RAPD technique was determined by the sequence of primer’s base and primer compound in each reaction. The aim of research was finding the best primer for genetic diversity analyses of Bitti (Vitex coffassus). The results of amplification showed that number of band between one to five bands (110-600 bp). The primer of OPK-10, OPA-17, OPQ-07 and OPP-08 can be used for genetic diversity analyses of Bitti. The best primer used for genetic diversity analyses of Bitti was OPP-08 because it had the highest number of bands (5 bands).
Key words: OPP-08, Primer Selection, Vitex coffassus
The species of trees have different secondary compounds that need optimum extraction techniques. Appropriate extraction techniques determine the quality and quantity of DNA produced. This research aims to found optimal of extraction methods and DNA isolation, then to created genome DNA in high quality and quantity, so that it can be using for genetic variation analyses in Suren (Toona sureni Merr.) by Random Amplified Polymorphic DNA (RAPD). This study shows that DNA concentrates were 763.3 μg/ml, 180.0 μg/ml, 383.3 μg/ml, and 436.7 μg/ml. While based on the results of PCR amplification using the primers OPD 03 shows that the four extraction methods used, the extraction method of number 3 has been able to produce genomic DNA with better quality and more number of bands, although the quantity is lower.
ABSTRAKSetiap jenis tanaman memiliki kandungan senyawa sekunder yang berbeda-beda sehingga membutuhkan teknik ekstraksi yang optimum. Teknik ekstraksi yang tepat sangat menentukan kualitas dan kuantitas DNA yang dihasilkan. Penelitian ini bertujuan untuk mendapatkan metode ekstraksi dan isolasi DNA yang optimal dan menghasilkan DNA genom yang berkualitas baik serta jumlah yang memadai sehingga dapat digunakan untuk analisis keragaman genetik pada tanaman suren (Toona sureni) berdasarkan Random Amplified Polymorphic DNA (RAPD). Hasil penelitian menunjukkan bahwa rata-rata konsentrasi DNA dihasilkan pada metode 1, 2, 3 dan 4 adalah berturut-turut 763,3 μg/ml; 180,0 μg/ml; 383,3 μg/ml dan 436,7 μg/ml. Berdasarkan hasil amplifikasi PCR menggunakan primer OPD 03 menunjukkan bahwa dari keempat metode ekstraksi yang digunakan, metode ekstraksi 3 mampu menghasilkan DNA genom dengan kualitas yang lebih baik dan jumlah pita yang lebih banyak walaupun kuantitas yang lebih rendah.
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