The Aldox and Adh structural loci of Musca domestica L. belong to autosome II. They code for the enzymes aldehyde oxidase and alcohol dehydrogenase. Both these enzymes have allelic variants with specific electrophoretic mobility, which, on cellogel, are seen as single bands. The Aldox and Adh loci encompass a large map interval, which includes the morphological markers at, cm, and car. The recombination frequencies between these five loci indicate the alignment Aldox -ar -cm -car -Adh.
Cellogel electrophoresis has been used to study isocitrate dehydrogenase (IDH), malate dehydrogenase (MDH), malic enzyme (ME), and alcohol dehydrogenase (ADH), in the housefly. Two forms of MDH (MDH, and MDH 2 ) have been separated. These show properties similar to those ascribed to supernatant and mitochondrial MDH enzymes in other organisms. In the housefly zymograms of the mitochondrial fraction show MDH 2 activity only. Allelic variants of MDH 1 differing in thermostability and resistance to p-hydroxymercuribenzoate have been revealed by qualitative analysis. MDH 2 is invariant. These findings indicate that the two forms are isozymes under independent genetic control.Other enzymes examined here exhibit only one isozyme, which shows genetic polymorphism in some strains.Using the variants and visible markers, the linkage groups of the structural loci of the enzymes have been identified: for some of them the genetic map position has been determined.
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