Germany). Abnormal thyroid function is common in takotsubo syndrome and depends on two distinct mechanisms: results of a multicentre observational study.

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Cows that received an over-calorie diet in the final phase of lactation are prone to liver lipidosis. The task of our research is to study the dynamics of cholesterol and triglycerides in cows with fatty hepatosis during the transit period. A group of 20 dry cows was formed. In the blood serum of cows, the concentration of cholesterol and triglycerides was studied twice before calving (15–20 days and 5–10 days) and twice after calving (5–10 days and 20–25 days). After research was formed two groups. The first group included 8 cows, which were killed 7–43 days after calving. They had signs of liver lipidosis. The diagnosis of fatty hepatosis was confirmed by the results of a histological examination of the liver. The second group included the remaining 12 cows. At the beginning of the study, the cows of the first and second groups had a cholesterol concentration of 4.06±0.23 and 3.62±0.23 mmol/l, and triglycerides - 0.13±0.01 and 0.11±0.01 mmol/l. Further dynamics of indicators had features in each group. In cows with hepatosis, a gradual decrease in cholesterol to 1.83±0.07 mmol/l was observed. In the second group the indicator was 2.2 times higher (P < 0.01). Before calving, a decrease in the concentration of triglycerides is observed. A more significant decline was detected in cows of the first group—by 61.5% (P < 0.001). In cows of the second group, the index decreased only by 18.2%. After 5–10 days after calving, the concentration of triglycerides in both groups remained low. After 20–25 days in the first group, the index increased 2.3 times (P < 0.01), and in the second—1.25 times. Studies have revealed features of the lipid profile in cows with liver lipidosis.

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When udder becomes inflamed during lactation, significant increase of somatic cells count in milk occurs. That is why an express-test based on surfactant’s effects was created. For this purpose 3 groups of samples were formed according to the somatic cell count in cm 3: group 1 - over 4.0*10^5 per cm3 (60 samples); group 2 - up to 4.0*10^5 in cm3 (100 samples); group 3 (control) - 3.0*10^5 in cm3 (30 samples). Experiments were conducted in the spring-summer and autumn-winter periods. Total amount of examined samples is 190. Somatic cell count in the samples was counted by viscometric method with the use of “Somatos-B.” We use the milk of animals 10–15 days after calving. Milk was mixed 1:1 with reagent, then after 3–4 tube shaking a height of foam column was determined. In the presence of somatic cells the foam forming is depressed. There was a positive reaction with column’s height from 3 to 10 (foaming) and negative reaction is considered to be from 0 to 3 (no foaming). There was a California mastitis test (CMT) as a control which is highly usable as an express-test in Leningrad region of Russia. The comparison of the results of two test show: on group 1 according to CMT 40 samples were positive, and foaming test determined 60 cases of somatic cells count increasing. On 2 group according to CMT 10 samples were positive and foaming test determined 60 cases of somatic cells count increasing. On 3 group according to CMT 1 sample was positive and foaming test not determined any somatic cells count increasing. This express method is not requires a big amount of time – it takes 8–10 seconds to mix a reagent and milk and evaluate results. So this method allows us to increase the mastitis diagnostic accuracy.