HighlightsWe have evaluated the role of carbon sources in controlling excessive phenolic secretion from callus culture of cotton.Maltose was found to control phenolic secretion when it is used as a sole carbon source.Efficient protocol was developed for high frequency callus induction and proliferation for SVPR-2 cotton cultivar using different plant growth regulators.Adjustment of carbon source concentrations was found to play an important role in cotton callus culture.The present work will be helpful for establishing callus culture and getting regeneration to carry out genetic transformation studies in SVPR-2 cotton cultivar.
Despite some perceptions of economic risk, the global aquaculture industry continues to attract new entrepreneurs and investors. However, to sustain this trend, innovative and cost-effective strategies are needed. In recent years, algal farming has been shown to be a sustainable activity in the global Blue Economy. In 2015, harvests of the red algal agarophyte genus Gracilaria surged to over 114,100 tons dry weight which constituted ca. 91% of the total raw material for industrial agar production. We carried out a trial of experimental farming of the agar-yielding alga known as Gracilaria dura using a tube-net method along the Gujarat coast, India. The mean daily growth rate (DGR) of this agarophyte ranged from 1.88 ± 0.23% day −1 to 3.30 ± 0.25% day −1. Similarly, the mean yield varied between 0.62 ± 0.03 kg and 1.05 ± 0.02 kg fresh wt m −1 of tube-net. A Pearson correlation showed that both DGR and biomass yield were positively correlated with temperature. In addition, the DGR was significantly positively correlated with air temperature (r = 0.916; p < 0.01) whereas biomass yield was also positively correlated with surface seawater temperature (r = 0.986; p < 0.001). The economic projections for four harvests per year revealed that the value of the product was US$5577, while the investment required for the necessary tube-net infrastructure was US$1797 thereby providing US $1890 as profit to single farmers, a significant amount in the coastal communities of Gujarat. Thus, undertaking commercial farming of G. dura in Indian waters could diversify the livelihood of artisanal, coastal fishermen and further encourage the indigenous production of agarose.
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