Evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (Gossypium hirsutum L.) cv. SVPR-2

Kumar, G. Prem; Shanmugam, Sivakumar; Govindarajan, Siva; Vinoth, S.; Manickam, V. S.; Mogilicherla, Kanakachari; Thiruppathi, S.; Jayabalan, N.

doi:10.1016/j.btre.2015.05.005

Cited by 37 publications

(19 citation statements)

References 29 publications

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“…The results (Table 1) show that the combination of 0.1 mg dm −3 KT + 0.025 mg dm −3 IBA induced the highest differentiation rate of cotton. Our callus differentiation rate statistics for exogenously applied auxin and cytokinin were consistent with previous studies, i.e., the induction of EC and SEs, and the concentration of 2, 4-D in the medium was replaced by a combination of IBA and KT [24,37]. In this experiment, the role of auxin and cytokinin was not achieved alone, but by interacting with other pathways and components involved in the differentiation and development of SEs, such as their upstream TF regulators and hormone signaling pathways [38].…”

Section: Iba Embryogenic Inductive Effect and Dose Effect During Diffsupporting

confidence: 89%

Dynamic Transcriptome Analysis Reveals Uncharacterized Complex Regulatory Pathway Underlying Dose IBA-Induced Embryogenic Redifferentiation in Cotton

Fan

Guo

et al. 2020

IJMS

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The somatic embryogenesis (SE) process of plants is regulated by exogenous hormones. During the SE, different genes sensitively respond to hormone signals through complex regulatory networks to exhibit plant totipotency. When cultured in indole-3-butyric acid (IBA) concentration gradient medium supplemented with 0 mg dm −3 , 0.025 mg dm −3 , and 0.05 mg dm −3 IBA, the callus differentiation rate first increased then decreased in cotton. To characterize the molecular basis of IBA-induced regulating SE, transcriptome analysis was conducted on embryogenic redifferentiation. Upon the examination of the IBA's embryogenic inductive effect, it was revealed that pathways related to plant hormone signal transduction and alcohol degradation were significantly enriched in the embryogenic responsive stage (5 days). The photosynthesis, alcohol metabolism and cell cycle pathways were specifically regulated in the pre-embryonic initial period (20 days). Upon the effect of the IBA dose, in the embryogenic responsive stage (5 days), the metabolism of xenobiotics by the cytochrome P450 pathway and secondary metabolism pathways of steroid, flavonoid, and anthocyanin biosynthesis were significantly enriched. The phenylpropanoid, brassinosteroid, and anthocyanin biosynthesis pathways were specifically associated in the pre-embryonic initial period (20 days). At different developmental stages of embryogenic induction, photosynthesis, flavonoid biosynthesis, phenylpropanoid biosynthesis, mitogen-activated protein kinase (MAPK) signaling, xenobiotics metabolism by cytochrome P450, and brassinosteroid biosynthesis pathways were enriched at low a IBA concentration. Meanwhile, at high IBA concentration, the carbon metabolism, alcohol degradation, circadian rhythm and biosynthesis of amino acids pathways were significantly enriched. The results reveal that complex regulating pathways participate in the process of IBA-induced redifferentiation in cotton somatic embryogenesis. In addition, collections of potential essential signaling and regulatory genes responsible for dose IBA-induced efficient embryogenic redifferentiation were identified. Quantitative real-time PCR (qRT-PCR) was performed on the candidate genes with different expression patterns, and the results are basically consistent with the RNA-seq data. The results suggest that the complicated and concerted IBA-induced mechanisms involving multiple cellular pathways are responsible for dose-dependent plant growth regulator-induced SE. This report represents a systematic study and provides new insight into molecular signaling and regulatory basis underlying the process of dose IBA-induced embryogenic redifferentiation during SE.

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Section: Iba Embryogenic Inductive Effect and Dose Effect During Diffsupporting

confidence: 89%

Dynamic Transcriptome Analysis Reveals Uncharacterized Complex Regulatory Pathway Underlying Dose IBA-Induced Embryogenic Redifferentiation in Cotton

Fan

Guo

et al. 2020

IJMS

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“…Rooting was declined vigorously when increasing (> 2.0 mg/L) concentrations of NAA. Similar results were also noticed in Sesamum indicum (Raja and Jayabalan, 2011), Solanum surattense (Rahman et al, 2011) and Gossypium hirsutum (Prem Kumar et al, 2015). …”

Section: In Vitro Rooting Of Shootssupporting

confidence: 78%

“…Other workers have also reported similar thick rosette types of shoots formation in higher concentrations of BA in case of Gossypium hirsutum (Prem Kumar et al, 2015; Commiphora wightii (Mohan et al, 2017). The shoot multiplication significantly varied according to the explant type.…”

Section: Shoot Multiplication From Leaf and Shoot Tip Explantssupporting

confidence: 60%

“…A high percentage of seed germination (90%) has been reported on sterile moistened filter paper or cotton in Petri dishes or test tubes in various crop species, including snake gourd (Pillai et al, 2008;Devendra et al, 2008 and; Brassica oleracea (Finch-Savage et al, 2006) and Triticum aestivum (Lehner et al, 2006) Ophiorrhiza mungos (Namdeo et al, 2012), Ipomoea sepiaria (Cheruvathur et al, 2015). In the present study, the higher regeneration efficiency was observed in leaf explants excised from 7-10 day-old seedlings (Gogoi et al, 2017), whereas in earlier reports cotyledon explants used were older than 3-4 days (Prem Kumar et al, 2015). Therefore, seeds were germinated on sterile moistened cotton and used for all the experiments in the present study.…”

Section: In Vitro Seedlingscontrasting

confidence: 52%

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In Vitro Plant Regeneration through Different Explant of Trichosanthes cucumerina L.

Muthuvel¹,

Vigneswaran²,

Jayabalan³

2017

Int. J. Curr. Res. Biosci. Plant Biol.

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“…In our experiment, maltose at a concentration of 90 g/L clearly reduced the L. aestivum plants' biomass. On the other hand, maltose at higher concentrations had a positive effect on F. indica and Gossypium hirsutum callus growth (Kumar et al, 2015;Khan et al, 2018).…”

Section: Discussionmentioning

confidence: 93%

Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis inLeucojum aestivumL. plants cultured in RITA^®bioreactor

Ptak

Morańska

Skrzypek

et al. 2020

PeerJ

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Background Leucojum aestivum L. is an important medicinal plant which produces Amaryllidaceae alkaloids, especially galanthamine and lycorine. Research is currently exploring the possibility of producing these alkaloids using biotechnological methods, including in vitro cultures. The biosynthesis of alkaloids may be affected by the types and concentrations of carbohydrate sources used in the medium. In the present investigation we performed such studies on in vitro cultures of L. aestivum with a view to obtaining plant material of good quality, characterized, in particular, by a high content of valuable Amaryllidaceae alkaloids. Methods We examined the effects of various types of carbohydrate sources—sucrose, glucose, fructose and maltose—at different concentrations (30, 60 and 90 g/L)—on the quality of L. aestivum plants grown in the RITA® bioreactor. The plants’ quality was assessed by their biomass increments, as well by as analysing photosynthetic pigments, endogenous sugar, phenolics and Amaryllidaceae alkaloid content. We also investigated the effect of sugars on the activity of the antioxidant enzymes catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD). Results The highest biomass increments were observed in plants cultivated in the medium containing 90 g/L sucrose. The highest CAT activity was noted in cultures growing in the medium supplemented with 90 g/L maltose, while the highest POD activity was observed in the presence of 90 g/L fructose and 60 g/L maltose. No differences in SOD activity were observed. Moreover, the sugars did not affect the contents of chlorophyll a and carotenoids, whereas the highest amount of chlorophyll b was recorded in plants growing in the medium with 60 g/L maltose. No statistically significant differences were observed in the contents of endogenous sugars and phenolics in any in vitro conditions. However, the addition of sugar had a decisive effect on the biosynthesis of the Amaryllidaceae alkaloids. The highest distribution of alkaloids occurred in plants cultured in the medium containing 60 g/L sucrose. Six Amaryllidaceae alkaloids were detected in the plant tissue. The addition of 30 g/L fructose in the medium resulted in the accumulation of five alkaloids, including ismine, which was not identified in other analysed tissues. The highest concentration of galanthamine was observed in plants cultured in the presence of 30 g/L fructose and 60 g/L sucrose (39.2 and 37.5 µg/g of dry weight (DW), respectively). The plants grown in the medium containing 60 g/L sucrose exhibited the highest lycorine content (1048 µg/g of DW). Conclusions The type and concentration of sugar used in the medium have an essential influence on the biosynthesis of Amaryllidaceae alkaloids in L. aestivum plants cultured in a RITA® bioreactor. The results point to an interesting approach for commercial production of galanthamine and lycorine.

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Evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (Gossypium hirsutum L.) cv. SVPR-2

Cited by 37 publications

References 29 publications

Dynamic Transcriptome Analysis Reveals Uncharacterized Complex Regulatory Pathway Underlying Dose IBA-Induced Embryogenic Redifferentiation in Cotton

Dynamic Transcriptome Analysis Reveals Uncharacterized Complex Regulatory Pathway Underlying Dose IBA-Induced Embryogenic Redifferentiation in Cotton

In Vitro Plant Regeneration through Different Explant of Trichosanthes cucumerina L.

Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis inLeucojum aestivumL. plants cultured in RITA^®bioreactor

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Evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (Gossypium hirsutum L.) cv. SVPR-2

Cited by 37 publications

References 29 publications

Dynamic Transcriptome Analysis Reveals Uncharacterized Complex Regulatory Pathway Underlying Dose IBA-Induced Embryogenic Redifferentiation in Cotton

Dynamic Transcriptome Analysis Reveals Uncharacterized Complex Regulatory Pathway Underlying Dose IBA-Induced Embryogenic Redifferentiation in Cotton

In Vitro Plant Regeneration through Different Explant of Trichosanthes cucumerina L.

Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis inLeucojum aestivumL. plants cultured in RITA®bioreactor

Contact Info

Product

Resources

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Carbohydrates stimulated Amaryllidaceae alkaloids biosynthesis inLeucojum aestivumL. plants cultured in RITA^®bioreactor