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The eco-friendly phytomediated synthesis of Ag nanoparticles provides better efficient applications than synthetic methods. Ag nanoparticles are considered by researchers due to its specific properties. The phytochemical analysis of Allium Sativum flower extract provides the phytochemicals exist in it. Due to this, the efficient eco-friendly synthesis of Ag nanoparticles via Allium Sativum flower extract has performed. The X-ray diffraction pattern exposes the crystalline nature with the mean crystallite size at 25 nm. The Fourier Transform Infra-Red spectrum displays the phytochemicals involved in the synthesis. The UV-Visible spectrum illustrates the formation of Ag nanoparticles with SPR band at 436 nm. The particle size analyzer demonstrates the size of formed Ag particles in 20-35 nm. The morphological, elemental and structural analyzes demonstrate the formation of spherical shaped Ag nanoparticles. The formation of Ag nanoparticles was confirmed by the appearance of Ag peak in Energy Dispersive X-ray Diffraction spectrum and mapping study. The antimicrobial activity explains the effective antimicrobial effect on human pathogens. The antioxidant activity reports the efficient scavenging property of synthesized Ag nanoparticles. The anti-inflammatory activity reveals the potential ability of protein denaturation on bovine serum albumin. The anti-diabetic activity exhibits much inhibition on α-Amylase. The anticancer activity reports the effective inhibition of synthesized Ag nanoparticles on human MCF-7 breast cancer cells.