“…Similar ndings have been reported in a study byMaheshwaran et al, where Z. rosea ower extract mediated Ag 2 O-NPs (100 µg/ml) showed 73.8% antioxidant activity thereby con rming the inhibition potential of Ag 2 O-NPs against reactive oxygen species (ROS)(Maheshwaran et al 2020). The increase in free-radical-scavenging activity of green amaranth mediated Ag 2 O-NPs with increasing concentration was equivalent to ascorbic acid.…”
In this study, silver oxide nanoparticles (Ag 2 O-NPs) were synthesized from silver nitrate using green amaranth leaf extract as reducing agent. The degradation of caffeine and inactivation of Escherichia coli by Ag 2 O-NPs was studied under compact uorescent lamp illumination irradiation. Apart from that, the antibacterial and antioxidant activities of Ag 2 O-NPs were also examined. Synthesized Ag 2 O-NPs were shaped like monodispersed husk, and cubic structured with surface area and average particle size was detected to be 100.21 (m 2 /g) and 81 nm respectively. Antioxidant e cacy of the Ag 2 O-NPs was evaluated using 1, 1-diphenyl-2-picrylhydrazyl and 91% inhibition was achieved with 100 µg Ag 2 O-NPs.Bacteriocidic propensity of Ag 2 O-NPs was examined against the S. aureus and P. aeruginosa by disc diffusion, minimum inhibitory concentration (MIC), Live and dead assay. It was observed that the NPs have higher bactericidal effect on Gram-negative as compared to Gram-positive bacteria. Up to 96% photocatalytic inactivation of E. coli was achieved using 30 µg/mL of NPs, Photocatalytic degradation of caffeine (50 ppm initial concentration) was observed to be 99% at pH 9 in 15 h using 50 mg/L of Ag 2 O NPs. These results indicate that Ag 2 O NPs can be employed in environmental applications like harmful bacteria inactivation and organic pollutants degradation.
“…Similar ndings have been reported in a study byMaheshwaran et al, where Z. rosea ower extract mediated Ag 2 O-NPs (100 µg/ml) showed 73.8% antioxidant activity thereby con rming the inhibition potential of Ag 2 O-NPs against reactive oxygen species (ROS)(Maheshwaran et al 2020). The increase in free-radical-scavenging activity of green amaranth mediated Ag 2 O-NPs with increasing concentration was equivalent to ascorbic acid.…”
In this study, silver oxide nanoparticles (Ag 2 O-NPs) were synthesized from silver nitrate using green amaranth leaf extract as reducing agent. The degradation of caffeine and inactivation of Escherichia coli by Ag 2 O-NPs was studied under compact uorescent lamp illumination irradiation. Apart from that, the antibacterial and antioxidant activities of Ag 2 O-NPs were also examined. Synthesized Ag 2 O-NPs were shaped like monodispersed husk, and cubic structured with surface area and average particle size was detected to be 100.21 (m 2 /g) and 81 nm respectively. Antioxidant e cacy of the Ag 2 O-NPs was evaluated using 1, 1-diphenyl-2-picrylhydrazyl and 91% inhibition was achieved with 100 µg Ag 2 O-NPs.Bacteriocidic propensity of Ag 2 O-NPs was examined against the S. aureus and P. aeruginosa by disc diffusion, minimum inhibitory concentration (MIC), Live and dead assay. It was observed that the NPs have higher bactericidal effect on Gram-negative as compared to Gram-positive bacteria. Up to 96% photocatalytic inactivation of E. coli was achieved using 30 µg/mL of NPs, Photocatalytic degradation of caffeine (50 ppm initial concentration) was observed to be 99% at pH 9 in 15 h using 50 mg/L of Ag 2 O NPs. These results indicate that Ag 2 O NPs can be employed in environmental applications like harmful bacteria inactivation and organic pollutants degradation.
“…A previous report provided evidence of the formation of AgNPs using the flower extract of Zephyranthes rosea. 26 The FTIR results indicated the presence of different functional groups in the extract that acted as reducing and stabilizing agents during the synthesis process of the NPs. The FTIR spectra of the AME extract and AME-AgNPs are shown in Figure 3A and B, respectively.…”
Section: Resultsmentioning
confidence: 99%
“…A previous report provided evidence of the formation of AgNPs using the flower extract of Zephyranthes rosea . 26 Figure 1 Photograph showing the change of colour due to AME-AgNPs formation. ( A ) AME flower extract, ( B ) mixed aqueous solution of AME flower extract and AgNo 3 and ( C ) formation of AgNPs.…”
Background: Abelmoschus esculentus (L.) Moench, an economically important malvaceous vegetable crop popularly known as okra, is used in various culinary preparations and is rich in vitamins, minerals, and nutrients. The biological properties of okra flowers in relation to nanoparticle synthesis have not yet been reported. Materials and Methods: In the current study, silver nanoparticles (AgNPs) were synthesized using extracts of the flowers of A. esculentus. The characteristics of the AgNPs were studied using a UV-vis spectrometer, Fourier transmission infrared spectrophotometer (FTIR), X-ray diffractometer (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM) and energy dispersive X-ray spectrometer (EDX). Antibacterial activity screening was performed using the agar well diffusion method, and cytotoxicity and cell viability studies were conducted using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The synthesized AgNPs were spherical and ranged in size from 5.52 to 31.96 nm, with an average size of 16.19 nm, as determined by UV-vis spectroscopy, FTIR, XRD, TEM and EDX. A. esculentus flower extract-mediated silver nanoparticles (AME-AgNPs) exhibited excellent activities in vitro studies, particularly in vitro cytotoxic and antiproliferative studies against cancer cell lines, such as the TERT-4 and A-549 cell lines. The antibacterial effects on the Gram-positive pathogens Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus pyogenes and the Gram-negative pathogens Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Salmonella typhimurium and Shigella sonnei were tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values varied with the bacterial strain. The IC 50 values of the synthesized NPs for the tested cell lines were close to that of a standard drug.
Conclusion:Compared to other NPs the NPs synthesized in this study were smaller in size and exhibited a higher level of antibacterial activity, cytotoxicity and apoptosis at minimal concentrations, and this is the first study on okra flower-induced anticancer and antimicrobial activities.
“…As seen in the Figure 2, DPPH activity increased with the increase in concentration as mentioned by other researchers. 20,21 In addition, the DPPH scavenging effect of Mp-AgNPs was compared with Ascorbic acid and Trolox, which were used as standard. According to the results of the study, the DPPH scavenging activity of Mp-AgNPs was found to be 38.64%, 49.30%, 56.83%, 81.80%, and 92.72% at 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L, and 200 mg/L concentrations, respectively.…”
Aim: Mushrooms known that to be used in traditional treatment among the people as they can generate a large diversity of secondary metabolites. In the present study, it was aimed to synthesized silver nanoparticles (AgNPs) mediated Macrolepiota procera, which is known to have diverse biological activities such as anticancer, antioxidant, antimicrobial in previous studies, and to investigated various in-vitro biological activities of these AgNPs. Materials and Methods: Synthesized Mp-AgNPs were characterized using transmission electron microscopy (TEM). Various biological activities including antimicrobial, biofilm inhibition and cell viability inhibition, DNA cleavage, DPPH activity of synthesized AgNPs were investigated in-vitro. Results: The highest DPPH scavenging activity of Mp-AgNPs was found as 92.72%, at 200 mg/L concentration. Mp-AgNPs caused single strain break in the E. coli pBR322 plasmid DNA. Mp-AgNPs showed moderate antimicrobial activity against tested microorganisms. Furthermore, the biofilm inhibition activity of Mp-AgNPs toward P. aeruginosa and S. aureus was 65.80% and 72.60% at 200 mg/L, respectively. Inhibition activity of E. coli cell viability of Mp-AgNPs was found as 99.99% at 500 mg/L. Conclusion: From the findings obtained in the study , the use of M. procera in the AgNPs synthesis it is important private regard due to its low cost, eco-friendly, high yield and non-toxicity human health. In addition, newly synthesized AgNPs can be used effectively for different applications after further studies.
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