Experiments were conducted to investigate the possibility of using progesterone releasing intravaginal devices (PRIDs) more than once, for the purpose of synchronising oestrus and ovulation in dairy cows. In an initial study, PRIDs were inserted into 6 ovariectomised cows for 12 days on 3 separate occasions and blood samples were collected for progresterone assay. After removal, 3 PRIDs were sterilised by autoclaving and the other 3 by gassing with ethylene oxide. After PRID insertion progesterone concentration in plasma rose rapidly. Autoclaved PRIDs which were reused once and then twice, maintained blood progesterone profiles which were comparable to the release of progesterone from a new PRID. This was not the case when PRIDs were re‐used after gas sterilisation.
In a second study, PRIDs were inserted into 41 dairy cows for 9 days and an injection of prostaglandin F2o was given one day before PRID removal. Onset of oestrus was determined by observation at intervals of 3 h for 30 min and time of ovulation was determined by endoscopy approximately 30 h after onset of oestrus. PRIDs were autoclaved after removal and re‐used twice.
In cows which received new PRIDs, 85% came into oestrus between 30 and 60 h after removal. When PRIDs were used for the second time, 100% of cows showed oestrus within 30 to 60 h. When PRIDs were used for the third time the interval between PRID removal and onset of oestrus was highly variable. Only 29% of cows showed oestrus within 30 to 60 h whereas 59% showed oestrus between 12 and 42 h after PRID removal.
The distribution in estimated time between PRID removal and ovulation followed closely the distribution of onset of oestrus for each insertion of PRIDs. The synchrony of ovulation was most concentrated for the second use of PRIDs and least for the third use. A few cows did not follow the general pattern of response.
Indications that the PRID may be used more than once for synchronising oestrus and ovulation in the dairy cow, and the adoption of a 9‐day PRID insertion interval in any synchronisation schedule should result in a significant reduction in the cost of this technology.
Melatonin pretreatment to improve the reproductive performance of first-cross Poll Dorset x Merino ewes joined in early summer was investigated in 2 experiments conducted in consecutive years. In experiment 1, melatonin pellets were administered subcutaneously to 1 of 3 groups of ewes; 14 days later they were joined with testosterone-primed wethers ('teasers'), followed after 15 days by entire rams (melatonin-teased group). The other groups were untreated and teased only. Experiment 2 consisted only of teased and melatonin-teased groups, with the ewes from experiment 1 being reallocated, as well as maiden ewes being joined for the first time. In this experiment melatonin pellets were inserted 38 days before the start of joining. Treatment with melatonin resulted in increased reproductive performances. The responses were characterised by an earlier (P<0.05) conception pattern in both experiments and higher (P<0.001) lambing percentages than controls in experiment 1. Up to 22% more lambs were born to melatonin-teased ewes than to teased ewes, and stimulatory effects were similar for ewes of different ages. In experiment 2, ewes that had received melatonin in both experiments tended (P>0.05) to have higher lambing percentages than teased ewes (up to 17% more lambs born), while ewes that had received melatonin in only experiment 1 tended (P>0.05) to have lower lambing percentages than ewes that were not treated with melatonin in either experiment (4-11%). The higher lambing performances of the melatonin-treated ewes were independent of ewe liveweights. Reproductive activity was also stimulated by the use of teasers alone in experiment 1. Conception patterns were advanced (P<0.01) and there was a trend for more (P>0.05) lambs to be born in the teased group than in the control group. The results show that both teasers and melatonin treatment will advance the conception patterns of first-cross Poll Dorset x Merino ewes joined in early summer, but the melatonin treatment also stimulated fecundity.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.