Multiple use of progesterone releasing intravaginal devices for synchronisation of oestrus and ovulation in cattle

Abstract

Experiments were conducted to investigate the possibility of using progesterone releasing intravaginal devices (PRIDs) more than once, for the purpose of synchronising oestrus and ovulation in dairy cows. In an initial study, PRIDs were inserted into 6 ovariectomised cows for 12 days on 3 separate occasions and blood samples were collected for progresterone assay. After removal, 3 PRIDs were sterilised by autoclaving and the other 3 by gassing with ethylene oxide. After PRID insertion progesterone concentration in plasma rose rapidly. Autoclaved PRIDs which were reused once and then twice, maintained blood progesterone profiles which were comparable to the release of progesterone from a new PRID. This was not the case when PRIDs were re‐used after gas sterilisation. In a second study, PRIDs were inserted into 41 dairy cows for 9 days and an injection of prostaglandin F2o was given one day before PRID removal. Onset of oestrus was determined by observation at intervals of 3 h for 30 min and time of ovulation was determined by endoscopy approximately 30 h after onset of oestrus. PRIDs were autoclaved after removal and re‐used twice. In cows which received new PRIDs, 85% came into oestrus between 30 and 60 h after removal. When PRIDs were used for the second time, 100% of cows showed oestrus within 30 to 60 h. When PRIDs were used for the third time the interval between PRID removal and onset of oestrus was highly variable. Only 29% of cows showed oestrus within 30 to 60 h whereas 59% showed oestrus between 12 and 42 h after PRID removal. The distribution in estimated time between PRID removal and ovulation followed closely the distribution of onset of oestrus for each insertion of PRIDs. The synchrony of ovulation was most concentrated for the second use of PRIDs and least for the third use. A few cows did not follow the general pattern of response. Indications that the PRID may be used more than once for synchronising oestrus and ovulation in the dairy cow, and the adoption of a 9‐day PRID insertion interval in any synchronisation schedule should result in a significant reduction in the cost of this technology.

Clinical Response of Ovarian Cysts in Dairy Cows after PRID Treatment.

“…Macmillan et al [18] observed that plasma progesterone concentrations fell rapidly after CIDR removal. Other studies observed similar progesterone concentrations at or near the end of progesterone treatment [8,20,30]. No significant difference in plasma progesterone on the day of PRID removal between cows that had recovered from follicular and luteal cysts, was observed in this study.…”

Reutilização de implantes de progesterona: do teste in vitro às taxas de concepção na IATF.

“…Essas constatações podem ajudar a evidenciar a presença de lacunas de P4 conforme o tempo de utilização do implante aumenta, uma vez que quando não há influência térmica, a liberação do fármaco é menor (McPHEE et al, 1983;LIU et al, 1985).…”